Spectroscopic characterization of PS I core complexes from thermophilic Synechococcus sp. Identical reoxidation kinetics of A1- before and after removal of the iron-sulfur-clusters FA and FB.

abstract：:Long noncoding RNA (lncRNA) are emerging as important regulators of diverse biological functions. Although mechanistic models are starting to emerge, it is also clear that the lncRNA field needs appropriate model systems in order to better elucidate the functions of lncRNA and their roles in both physiological and pat...

journal_title：FEBS letters

authors： Leone S,Santoro R

更新日期：2016-08-01 00:00:00

abstract：:Electrospray/mass spectrometry (ES/MS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates cont...

journal_title：FEBS letters

authors： Torella C,Ruoppolo M,Marino G,Pucci P

更新日期：1994-10-03 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:Microinjection of human liver mRNA from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2--10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (alpha 1-antitrypsin PiMM), or water was injected into t...

journal_title：FEBS letters

authors： Bathurst IC,Errington DM,Foreman RC,Judah JD,Carrell RW

更新日期：1985-04-22 00:00:00

abstract：:The use of oryzacystatins I and II, two cysteine proteinase inhibitors naturally produced in rice grains, represents an attractive way for the control of Coleoptera insect pests. The present study was done to analyze the inhibitory effect of recombinant oryzacystatins produced in Escherichia coli as fusion proteins ag...

journal_title：FEBS letters

authors： Michaud D,Nguyen-Quoc B,Yelle S

更新日期：1993-09-27 00:00:00

abstract：:A comparative study has investigated the in vitro dissociation and self-assembly of chaperonin 60 14-mers isolated from E. coli (GroEL), yeast mitochondria and pea chloroplasts. In all cases Mg2+ inhibits, and low temperature stimulates, the urea-induced dissociation. ATP or ADP in the presence of Mg2+ enhance the dis...

journal_title：FEBS letters

authors： Lissin NM

更新日期：1995-03-13 00:00:00

abstract：:IGF-I receptors were partially purified from red and white skeletal muscle by lectin-affinity chromatography and the resultant fraction was depleted of insulin receptors by insulin affinity chromatography. Equilibrium binding of 125I-IGF-I to receptor preparations from red and white muscle yielded identical Scatchard ...

journal_title：FEBS letters

authors： Zorzano A,James DE,Ruderman NB,Pilch PF

更新日期：1988-07-18 00:00:00

abstract：:Escherichia coli heat-shock proteins GroEL and GroES stimulate (in an ATP-dependent manner) the folding of various proteins. In this study scanning microcalorimetry was applied to investigate GroEL thermostability in the presence of its ligands. Mg2+ and K+ ions stabilize while ADP destabilizes the GroEL molecule agai...

journal_title：FEBS letters

authors： Surin AK,Kotova NV,Kashparov IA,Marchenkov VV,Marchenkova SYu,Semisotnov GV

更新日期：1997-04-01 00:00:00

abstract：:The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...

journal_title：FEBS letters

authors： Madern D,Anselme J,Härtlein M

更新日期：1992-03-24 00:00:00

abstract：:The rate at which dinucleoside phosphates are cleaved by RNases is supposed to be determined by the mole fraction of enzyme-substrate complexes in which the phosphodiester moiety of a dinucleoside phosphate has a highly reactive conformation. The mole fraction of such complexes for a particular RNase depends on the na...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP,Mikhaylov SN

更新日期：1985-01-07 00:00:00

abstract：:The aspA gene, encoding the aspartyl-tRNA synthetase (AspRS) from the hyperthermophilic archaeon Pyrococcus sp. KOD1, was expressed in Escherichia coli. The KOD1 AspRS, which was purified to homogeneity and was shown to be functional in dimeric form, aminoacylated tRNA from KOD1. The optimum temperature for this activ...

journal_title：FEBS letters

authors： Fujiwara S,Lee SG,Haruki M,Kanaya S,Takagi M,Imanaka T

更新日期：1996-09-23 00:00:00

abstract：:Interactions of U2 snRNA with anti-Sm precipitable proteins in RNA-free cytoplasmic complexes were analyzed. U2 snRNA was found to bind specifically with proteins in the 6S complexes but not in the 20S complexes. The binding activity was preserved using U2 snRNA having a mutated Sm binding site. Label-transfer experim...

journal_title：FEBS letters

authors： Fury MG,Andersen J

更新日期：1997-03-03 00:00:00

abstract：:Single enzyme molecules display inevitable, stochastic fluctuations in their catalytic activity. In metabolism, for instance, the stochastic activity of individual enzymes is averaged out due to their high copy numbers per single cell. However, many processes inside cells rely on single enzyme activity, such as transc...

journal_title：FEBS letters

authors： Schwabe A,Maarleveld TR,Bruggeman FJ

更新日期：2013-09-02 00:00:00

abstract：:Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

journal_title：FEBS letters

authors： Keil P,Pfanner N

更新日期：1993-04-26 00:00:00

abstract：:Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20...

journal_title：FEBS letters

authors： Boros S,Kamps B,Wunderink L,de Bruijn W,de Jong WW,Boelens WC

更新日期：2004-10-08 00:00:00

abstract：:Metabolic activities of orexin A (OXA) in mature adipocytes are mediated via PI3K/PKB and PPARγ. However, the effects of OXA on preadipocytes are largely unknown. We report here that OXA stimulates the proliferation and viability of 3T3-L1 preadipocytes and protects them from apoptosis via ERK1/2, but not through PKB....

journal_title：FEBS letters

authors： Skrzypski M,Kaczmarek P,Le TT,Wojciechowicz T,Pruszyńska-Oszmalek E,Szczepankiewicz D,Sassek M,Arafat A,Wiedenmann B,Nowak KW,Strowski MZ

更新日期：2012-11-30 00:00:00

abstract：:The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

journal_title：FEBS letters

authors： Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

更新日期：2001-08-24 00:00:00

abstract：:The molecular mass of the rabbit prolactin receptor (rbPRLR) deduced from cDNA cloning is 66 kDa. However, the molecular mass of the full-length receptor expressed in the insect Sf9 cells was found to be 94 kDa. In order to explain this discrepancy, we analyzed the possible post-translational modifications of the PRLR...

journal_title：FEBS letters

authors： Cahoreau C,Garnier L,Djiane J,Devauchelle G,Cerutti M

更新日期：1994-08-22 00:00:00

abstract：:Cytosine methylation of binding sites for transcription factors is a straightforward mechanism to prevent transcription, while data on an indirect mechanism, by methylation outside of the factor binding sites, are still scarce. We have studied the latter effect using a model promoter construct. For this, a 69 bp G + C...

journal_title：FEBS letters

authors： Hug M,Silke J,Georgiev O,Rusconi S,Schaffner W,Matsuo K

更新日期：1996-02-05 00:00:00

abstract：:Molecular dynamics analyses were performed to examine conformational changes in the C-domain of calmodulin and the N-domain of troponin C induced by binding of Ca(2+) ions. Analyses of conformational changes in calmodulin and troponin C indicated that the shortening of the distance between Ca(2+) ions and Ca(2+) bindi...

journal_title：FEBS letters

authors： Goto K,Toyama A,Takeuchi H,Takayama K,Saito T,Iwamoto M,Yeh JZ,Narahashi T

更新日期：2004-03-12 00:00:00

abstract：:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

journal_title：FEBS letters

authors： Allwood EG,Smertenko AP,Hussey PJ

更新日期：2001-06-15 00:00:00

abstract：:The guanine nucleotide exchange reaction catalyzed by elongation factor Ts is proposed to arise from the intrusion of the side chains of D80 and F81 near the Mg2+ binding site in EF-Tu. D80A and F81A mutants of E. coli EF-Ts were 2-3-fold less active in promoting GDP exchange with E. coli EF-Tu while the D80AF81A muta...

journal_title：FEBS letters

authors： Zhang Y,Li X,Spremulli LL

更新日期：1996-08-12 00:00:00

abstract：:Published amino acid sequences for nitrogenase component polypeptides were compared with those of other proteins which also bind adenine nucleotides. Three sequences which might contribute to an adenine nucleotide-binding domain were found for the Fe-protein component of nitrogenase. The beta-subunit of the MoFe-prote...

journal_title：FEBS letters

authors： Robson RL

更新日期：1984-08-06 00:00:00

abstract：:Aminoacyl-tRNA synthetases, a group of enzymes catalyzing aminoacyl-tRNA formation, may possess inherent editing activity to clear mistakes arising through the selection of non-cognate amino acid. It is generally assumed that both editing substrates, non-cognate aminoacyl-adenylate and misacylated tRNA, are hydrolyzed...

journal_title：FEBS letters

authors： Gruic-Sovulj I,Rokov-Plavec J,Weygand-Durasevic I

更新日期：2007-10-30 00:00:00

abstract：:Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factors VIIa and Xa through Kunitz domains, thereby inhibiting the activity of tissue factor. However, it has been shown that the C-terminal of this inhibitor is essential for the maximal anticoagulant activity of TFPI. We have investigated th...

journal_title：FEBS letters

authors： Ettelaie C,Adam JM,James NJ,Oke AO,Harrison JA,Bunce TD,Bruckdorfer KR

更新日期：1999-12-17 00:00:00

abstract：:The number of proteins found in body fluids and at cell surfaces, which are known to display carbohydrate-binding properties, continues to increase rapidly. In these proteins, in addition to a domain associated with lectin properties, one or more, non-lectin domains are present. It is possible that binding of sugar re...

journal_title：FEBS letters

authors： Thiel S,Reid KB

更新日期：1989-06-19 00:00:00

abstract：:Accurate cDNA data is useful to validate gene structures in a genome. We sequenced 35189 expressed sequence tags (ESTs) obtained from the highly destructive rice blast fungus, Magnaporthe grisea. Our custom-made computational programs mapped these ESTs on the M. grisea genome sequence, and reconstructed gene structure...

journal_title：FEBS letters

authors： Numa H,Nishimura M,Tanaka T,Kanamori H,Yang CC,Matsumoto T,Nagamura Y,Itoh T

更新日期：2009-02-18 00:00:00

abstract：:A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and ...

journal_title：FEBS letters

authors： Tagaya M,Henomatsu N,Yoshimori T,Yamamoto A,Tashiro Y,Fukui T

更新日期：1993-06-14 00:00:00

abstract：:In presynaptic nerve terminals, synaptic vesicles are recycled locally via an evolutionarily conserved process that ensures maintenance of neurotransmission as well as structural integrity of synapses. Temperature is a key environmental factor that impacts critical steps involved in fusion, endocytosis and transport i...

journal_title：FEBS letters

authors： Chanaday NL,Kavalali ET

更新日期：2018-11-01 00:00:00