Evidence of a tissue-restricting DNA regulatory element in the mouse IRBP promoter.

abstract：:The possible molecular heterogeneity of human transferrin receptors was analyzed using two murine monoclonal antibodies, Tü15 and Tü67. Both reagents precipitated from lysates of 125I-labeled HL-60 cells a major component of 88 kDa which could be identified as the transferrin receptor by comparison with the proteins d...

journal_title：FEBS letters

authors： Uchańska-Ziegler B,Wernet P,Liangru S,Ziegler A

更新日期：1984-10-01 00:00:00

abstract：:Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant p...

journal_title：FEBS letters

authors： Hannauer M,Schäfer M,Hoegy F,Gizzi P,Wehrung P,Mislin GL,Budzikiewicz H,Schalk IJ

更新日期：2012-01-02 00:00:00

abstract：:We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish. As the protein of 222 amino acids is similar to Brorin, a secreted BMP antagonist, which is a member of the Chordin family, we named it Brorin-like. Recombinant Brorin-like protein weakly but significantly inhibited the activity of ...

journal_title：FEBS letters

authors： Miwa H,Miyake A,Kouta Y,Shimada A,Yamashita Y,Nakayama Y,Yamauchi H,Konishi M,Itoh N

更新日期：2009-11-19 00:00:00

abstract：:We have found that SV40 infection of CV1 cells induces the synthesis of a 72 kDa protein that upon molecular cloning was shown to be the product of the hsc70 gene. The above gene product was found to be mainly virus inducible, in contrast to the hsp70 gene product which was mainly heat inducible. The two genes were fo...

journal_title：FEBS letters

authors： Sainis I,Angelidis C,Pagoulatos G,Lazaridis I

更新日期：1994-12-05 00:00:00

abstract：:MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF form...

journal_title：FEBS letters

authors： Tanaka S,Robinson EA,Yoshimura T,Matsushima K,Leonard EJ,Appella E

更新日期：1988-08-29 00:00:00

abstract：:It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP

更新日期：1984-10-01 00:00:00

abstract：:The central nervous system of the pond snail, Lymnaea stagnalis, contains many large, identified neurons which can be easily manipulated making it an advantageous model system to elucidate in vivo the architecture of neuronal signal transduction pathways. We have isolated three cDNA clones encoding G protein alpha sub...

journal_title：FEBS letters

authors： Knol JC,Weidemann W,Planta RJ,Vreugdenhil E,van Heerikhuizen H

更新日期：1992-12-21 00:00:00

abstract：:Incubation of isolated hepatocytes containing normal Ca2+ levels with angiotensin II, vasopressin or A23187 caused significant inhibition of the cAMP response to glucagon. Angiotensin II also inhibited cAMP accumulation induced by either glucagon or epinephrine in Ca2+-depleted hepatocytes. When submaximal doses of ho...

journal_title：FEBS letters

authors： Morgan NG,Exton JH,Blackmore PF

更新日期：1983-03-07 00:00:00

abstract：:Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...

journal_title：FEBS letters

authors： Iswari S,Krishnamurthy DS,Jacob TM

更新日期：1985-09-23 00:00:00

abstract：:A stabilization of both the precursor and the mature beta 1 integrin subunit was observed in metabolically labeled human skin fibroblasts and Molt-4 T lymphocytes upon addition of protein synthesis inhibitors or by ATP depletion. Differential effects of protein synthesis inhibitors are reported since the slow degradat...

journal_title：FEBS letters

authors： Vekeman S,Jaspers M,Cassiman JJ

更新日期：1993-07-26 00:00:00

abstract：:A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...

journal_title：FEBS letters

authors： Law GJ,Ray KP,Wallis M

更新日期：1984-01-23 00:00:00

abstract：:Electrospray/mass spectrometry (ES/MS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates cont...

journal_title：FEBS letters

authors： Torella C,Ruoppolo M,Marino G,Pucci P

更新日期：1994-10-03 00:00:00

abstract：:The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...

journal_title：FEBS letters

authors： Gibson LC,Hunter CN

更新日期：1994-09-26 00:00:00

abstract：:The dual function of the regulatory beta-subunit of protein kinase CK2 is highlighted by its ability to abolish calmodulin phosphorylation in contrast to its stimulatory effect on the phosphorylation of peptide substrates. Here we show that a synthetic peptide reproducing the C-terminal region of the beta-subunit (bet...

journal_title：FEBS letters

authors： Marin O,Meggio F,Boldyreff B,Issinger OG,Pinna LA

更新日期：1995-04-17 00:00:00

abstract：:CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...

journal_title：FEBS letters

authors： Ollila J,Lappalainen I,Vihinen M

更新日期：1996-11-04 00:00:00

abstract：:Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...

journal_title：FEBS letters

authors： Habu N,Samejima M,Dean JF,Eriksson KE

更新日期：1993-07-26 00:00:00

abstract：:This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyz...

journal_title：FEBS letters

authors： Gopaul NK,Anggård EE,Mallet AI,Betteridge DJ,Wolff SP,Nourooz-Zadeh J

更新日期：1995-07-17 00:00:00

abstract：:Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

journal_title：FEBS letters

authors： Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

更新日期：2005-08-15 00:00:00

abstract：:The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport hexoses. In a yeast strain deleted for HXT1-17, GAL2...

journal_title：FEBS letters

authors： Wieczorke R,Krampe S,Weierstall T,Freidel K,Hollenberg CP,Boles E

更新日期：1999-12-31 00:00:00

abstract：:We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...

journal_title：FEBS letters

authors： Yoon JH,Lee HS,Kim TH,Woo GH,Kim CY

更新日期：2000-06-02 00:00:00

abstract：:The structural basis of GABA-gated chloride channels in mammalian brain is presently explored by the functional expression of cDNAs coding for the alpha, beta or gamma-subunits of the receptor and their isoforms. In this context, we expressed the cloned cDNA coding for the rat beta 1-subunit of the GABAA receptor in t...

journal_title：FEBS letters

authors： Sigel E,Baur R,Malherbe P,Möhler H

更新日期：1989-11-06 00:00:00

abstract：:We report the identification, gene expression and biological activity of two parathyroid hormones (PTH; PTHA and PTHB), two PTH-related peptides (PTHrP; PTHrPA and PTHrPB) and a PTH-like ligand (PTH-L) with hybrid characteristics in puffer fishes (Takifugu rubripes and Tetraodon fluviatilis). Experimental data are con...

journal_title：FEBS letters

authors： Canario AV,Rotllant J,Fuentes J,Guerreiro PM,Rita Teodósio H,Power DM,Clark MS

更新日期：2006-01-09 00:00:00

abstract：:To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from ph...

journal_title：FEBS letters

authors： Nord M,Gustafsson JA,Lund J

更新日期：1995-11-06 00:00:00

abstract：:Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1....

journal_title：FEBS letters

authors： Pomar F,Caballero N,Pedreño M,Ros Barceló A

更新日期：2002-10-09 00:00:00

abstract：:Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC coul...

journal_title：FEBS letters

authors： Monemi M,Eriksson PO,Dubail I,Butler-Browne GS,Thornell LE

更新日期：1996-05-13 00:00:00

abstract：:Neprilysin-2 (NEP2) is a novel metallopeptidase homologous to neprilysin (NEP), an enzyme involved in regulation of neuropeptide signalling. NEP2 exists as two alternatively spliced isoforms, NEP2 and NEP2(Delta). In this study, we cloned and expressed both human isoforms. Human NEP2 exists as a membrane-bound and sol...

journal_title：FEBS letters

authors： Whyteside AR,Turner AJ

更新日期：2008-07-09 00:00:00

abstract：:Granulocyte colony-stimulating factor (G-CSF) has been recently shown to induce the high-affinity Fc receptor for IgG (Fc(gammaRI)/CD64) in human polymorphonuclear neutrophils (PMN). To elucidate the molecular mechanisms whereby G-CSF exerts this effect, we examined whether the cytokine induces the binding of transcri...

journal_title：FEBS letters

authors： Bovolenta C,Gasperini S,Cassatella MA

更新日期：1996-05-20 00:00:00

abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

journal_title：FEBS letters

authors： Hinsley AP,Stanley NR,Palmer T,Berks BC

更新日期：2001-05-18 00:00:00

abstract：:The prohormone convertase PC2 is one of the major subtilisin/kexin-like enzymes responsible for the formation of small bioactive peptides in neural and endocrine cells. This convertase is unique among the members of the subtilisin/kexin-like mammalian serine proteinase family in that it undergoes zymogen processing of...

journal_title：FEBS letters

authors： Benjannet S,Mamarbachi AM,Hamelin J,Savaria D,Munzer JS,Chrétien M,Seidah NG

更新日期：1998-05-22 00:00:00

abstract：:The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...

journal_title：FEBS letters

authors： Olds RJ,Lane DA,Boisclair M,Sas G,Bock SC,Thein SL

更新日期：1992-04-06 00:00:00