Abstract:
:Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the two types of SerRSs also differ in their recognition of the serine substrate, we compared the sensitivity of the representative methanogenic and bacterial-type SerRSs to serine hydroxamate and two previously unidentified inhibitors, serinamide and serine methyl ester. Our kinetic data showed selective inhibition of the methanogenic SerRS by serinamide, suggesting a lack of mechanistic uniformity in serine recognition between the evolutionarily distinct SerRSs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic Idoi
10.1016/j.febslet.2005.06.073keywords:
subject
Has Abstractpub_date
2005-08-15 00:00:00pages
4344-8issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00826-4journal_volume
579pub_type
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