Abstract:
:The structural basis of GABA-gated chloride channels in mammalian brain is presently explored by the functional expression of cDNAs coding for the alpha, beta or gamma-subunits of the receptor and their isoforms. In this context, we expressed the cloned cDNA coding for the rat beta 1-subunit of the GABAA receptor in the Xenopus oocyte. Surprisingly, efficient expression of a functional ion channel was found. The channel was anion-selective, and able to open in the absence of GABA. Since this channel could be shunt by the GABA-channel blocker picrotoxin, we conclude that the beta 1-subunit of the GABAA receptor is sufficient to form binding sites for picrotoxin.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sigel E,Baur R,Malherbe P,Möhler Hdoi
10.1016/0014-5793(89)81576-5subject
Has Abstractpub_date
1989-11-06 00:00:00pages
377-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81576-5journal_volume
257pub_type
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