Lack of adenosine deaminase deficiency in the mutant mouse wasted.


:The possibility that the mutant mouse wasted (wst/wst) may serve as an animal model for studies of severe combined immunodeficiency disease (SCID) and the role of adenosine deaminase (ADA, EC in adenosine metabolism were investigated. The specific activity of ADA in wst/wst compared with control mice was significantly lower by 26% in thymus, but significantly higher by 18% in spleen and 32% in cerebellum. Vmax values of ADA in spleens were 43% higher in wst/wst mice and no changes were observed in Km values. In contrast, the Vmax of ADA was unchanged in erythrocytes from wst/wst mice, but the Km for adenosine was significantly elevated. Thus, based on ADA measurements alone, it may be premature to consider wst/wst mice as a model for ADA deficiency and SCID in humans.




FEBS letters


Geiger JD,Nagy JI




Has Abstract


1986-11-24 00:00:00














  • Cloning and expression of the hydrogenase gene from Clostridium butyricum in Escherichia coli.

    abstract::Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

    更新日期:1983-07-11 00:00:00

  • Nop5 interacts with the archaeal RNA exosome.

    abstract::The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Gauernack AS,Lassek C,Hou L,Dzieciolowski J,Evguenieva-Hackenberg E,Klug G

    更新日期:2017-12-01 00:00:00

  • Synthesis and biological evaluation of substituted phosphate triester alkyl lyso phospholipids (ALPs) as novel potential anti-neoplastic agents.

    abstract::Phosphate triester derivatives of the anti-neoplastic alkyl lyso phospholipid (ALP) have been prepared as novel potential therapeutic agents. In particular, symmetrical phosphate triesters have been prepared, using phosphorochloridate chemistry. The compounds have been fully characterised by a range of techniques, and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McGuigan C,Wang Y,Riley PA

    更新日期:1995-09-25 00:00:00

  • The constituents of storage granules in the dermal glands of Xenopus laevis. Structure of a basic polypeptide deduced from cloned cDNA.

    abstract::Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berger H,Kreil G

    更新日期:1989-06-05 00:00:00

  • Inhibition of creatine kinase by S-nitrosoglutathione.

    abstract::The sarcoplasmic reticulum-bound creatine kinase from rabbit skeletal muscle was inhibited by the nitric oxide donor S-nitrosoglutathione (GSNO). This led to a decrease in Ca2+ uptake in sarcoplasmic reticulum vesicles when the transport was driven by ATP generated from phosphocreatine and ADP. In contrast, the Ca 2+ ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wolosker H,Panizzutti R,Engelender S

    更新日期:1996-09-02 00:00:00

  • Role of inward K+ channel located at carrot plasma membrane in signal cross-talking of cAMP with Ca2+ cascade.

    abstract::Treatment of cultured carrot cells with dibutyryl cAMP or forskolin resulted in the appreciable decrease in extracellular K+ concentration. This decrease was found to be transient and the concentration of the ion in the culture medium restored to the original level within few minutes. The cAMP-induced decrease in K+ l...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kurosaki F

    更新日期:1997-05-12 00:00:00

  • Regulation of oxygen consumption and microcirculation by alpha-sympathetic nerves in isolated perfused rat liver.

    abstract::In isolated rat liver perfused at constant flow with erythrocyte-free Krebs-Henseleit bicarbonate buffer containing 5 mM glucose and 2 mM lactate, perivascular stimulation of the hepatic nerves caused a rapid decrease of oxygen uptake, a decrease of the periportal and, after a transient rise, of the perivenous tissue ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ji S,Beckh K,Jungermann K

    更新日期:1984-02-13 00:00:00

  • Phorbol-ester-induced phosphorylation of the beta 2-adrenergic receptor decreases its coupling to Gs.

    abstract::Phorbol-esters have been shown to modulate the beta-adrenergic-stimulated adenylyl cyclase in a number of cell lines. Here, using site directed mutagenesis, we investigate the role of the beta 2-adrenergic receptor phosphorylation by protein kinase C in this regulatory process. Mutation of the serine-261, -262, -344 a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bouvier M,Guilbault N,Bonin H

    更新日期:1991-02-25 00:00:00

  • Voltage-gated K+ currents of mouse dendritic cells.

    abstract::Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fischer HG,Eder C

    更新日期:1995-10-09 00:00:00

  • Crystallization and preliminary X-ray crystallographic study of interleukin-8.

    abstract::Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

    更新日期:1990-06-04 00:00:00

  • Suppression of HIV-1 transcription by beta-chemokines RANTES, MIP1-alpha, and MIP-1beta is not mediated by the NFAT-1 enhancer element.

    abstract::Soluble factors derived from human CD8+ T-lymphocytes inhibit HIV-1 replication by suppressing transcription from the viral long terminal repeat (LTR), an effect shown to be mediated in part by an NFAT-1 enhancer sequence. We show here that the CD8+ derived beta-chemokines, RANTES, MIP1-alpha, and MIP-1beta, known sup...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Handen JS,Rosenberg HF

    更新日期:1997-06-30 00:00:00

  • Brefeldin A and the endocytic pathway. Possible implications for membrane traffic and sorting.

    abstract::A number of recent observations have suggested that the endocytic and biosynthetic pathways may share fundamentally similar transport mechanisms at the molecular level. Some of the more striking of these suggestions have come from a comparison of the effects of the macrocyclic lactone brefeldin A (BFA) on endosomes an...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Hunziker W,Whitney JA,Mellman I

    更新日期:1992-07-27 00:00:00

  • Iron binding to microsomes and liposomes in relation to lipid peroxidation.

    abstract::The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vile GF,Winterbourn CC

    更新日期:1987-05-04 00:00:00

  • Glycosyltransferase complexes improve glycolipid synthesis.

    abstract::The synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Spessott W,Crespo PM,Daniotti JL,Maccioni HJ

    更新日期:2012-07-30 00:00:00

  • Chemotactic and osmotic signals share a cGMP transduction pathway in Dictyostelium discoideum.

    abstract::In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kuwayama H,Van Haastert PJ

    更新日期:1998-03-13 00:00:00

  • High-level production and isolation of human recombinant alpha 1-proteinase inhibitor in yeast.

    abstract::The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

    更新日期:1986-08-11 00:00:00

  • NPC1L1 and cholesterol transport.

    abstract::The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Betters JL,Yu L

    更新日期:2010-07-02 00:00:00

  • Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae.

    abstract::The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport hexoses. In a yeast strain deleted for HXT1-17, GAL2...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wieczorke R,Krampe S,Weierstall T,Freidel K,Hollenberg CP,Boles E

    更新日期:1999-12-31 00:00:00

  • Studies on the maintenance of cytochromes P-450 and b5, monooxygenases and cytochrome reductases in primary cultures of rat hepatocytes.

    abstract::The cytochrome P-450 content of rat hepatocytes declined rapidly over 72 h in culture, due primarily to denaturation to cytochrome P-420. Six different media were investigated for their ability to conserve cytochrome P-450 during culture, and the most successful was a modified Earle's medium. After 72 h culture in thi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Grant MH,Melvin MA,Shaw P,Melvin WT,Burke MD

    更新日期:1985-10-07 00:00:00

  • Extended binding sites of cyclophilin as revealed by the interaction with HIV-1 Gag polyprotein derived oligopeptides.

    abstract::Oligopeptides derived from the gag polyprotein (Pr55gag) of human immunodeficiency virus type 1 (HIV-1) segment were used to evaluate the extension of the putative binding region for the complex of Pr55gag and the human cytosolic peptidyl prolyl cis/trans isomerase (PPIase) 18 kDa cyclophilin (Cyp18). Five N-terminall...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schutkowski M,Drewello M,Wöllner S,Jakob M,Reimer U,Scherer G,Schierhorn A,Fischer G

    更新日期:1996-10-07 00:00:00

  • Purification of Escherichia coli 50 S ribosomal proteins by high performance liquid chromatography.

    abstract::The 50 S subunit proteins from the Escherichia coli ribosome were purified by size-exclusion, ion-exchange or reversed phase high performance liquid chromatography (HPLC) avoiding any precipitation or desalting procedures during isolation. Best resolution of this complex protein mixture was achieved by reversed phase ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kamp RM,Wittmann-Liebold B

    更新日期:1984-02-13 00:00:00

  • Two sites of primary degradation of the D1-protein induced by acceptor or donor side photo-inhibition in photosystem II core complexes.

    abstract::Depending on experimental conditions we have found that photo-inhibitory treatment of photosystem II (PSII) core complexes, isolated from wheat, can generate two fragments of about 23-24 kDa that contain either the C-terminal or N-terminal regions of the D1-protein. A 24 kDa C-terminal fragment appears when the water ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: De Las Rivas J,Andersson B,Barber J

    更新日期:1992-04-27 00:00:00

  • New muteins of RNase A with enhanced antitumor action.

    abstract::Monomeric bovine pancreatic RNase A has been transformed into a dimeric ribonuclease with antitumor activity (Di Donato, A., Cafaro, V. and D'Alessio, G. (1994) J. Biol. Chem. 269, 17394-17396). This was accomplished by replacing the residues located in the RNase chain at positions 19, 28, 31, and 32, with proline, le...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cafaro V,Bracale A,Di Maro A,Sorrentino S,D'Alessio G,Di Donato A

    更新日期:1998-10-16 00:00:00

  • Control of high osmolarity signalling in the yeast Saccharomyces cerevisiae.

    abstract::Signal transduction pathways control cellular responses to extrinsic and intrinsic signals. The yeast HOG (High Osmolarity Glycerol) response pathway mediates cellular adaptation to hyperosmotic stress. Pathway architecture as well as the flow of signal have been studied to a very high degree of detail. Recently, the ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Hohmann S

    更新日期:2009-12-17 00:00:00

  • Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody.

    abstract::Mitogen-activated protein (MAP) kinases p42mapk and p44mapk are activated by dual tyrosine and threonine phosphorylation in vivo. Both MAPKs are phosphorylated and activated in vitro by an activator recently identified as a protein-tyrosine/threonine kinase. We have isolated a putative cDNA for a MAP kinase kinase (MA...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Haystead CM,Wu J,Gregory P,Sturgill TW,Haystead TA

    更新日期:1993-02-08 00:00:00

  • Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast.

    abstract::We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aerts AM,Zabrocki P,Govaert G,Mathys J,Carmona-Gutierrez D,Madeo F,Winderickx J,Cammue BP,Thevissen K

    更新日期:2009-01-05 00:00:00

  • p53: the barrier to cancer stem cell formation.

    abstract::The role of p53 as the "guardian of the genome" in differentiated somatic cells, triggering various biological processes, is well established. Recent studies in the stem cell field have highlighted a profound role of p53 in stem cell biology as well. These studies, combined with basic data obtained 20 years ago, provi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aloni-Grinstein R,Shetzer Y,Kaufman T,Rotter V

    更新日期:2014-08-19 00:00:00

  • Synthesis of homocysteine thiolactone by methionyl-tRNA synthetase in cultured mammalian cells.

    abstract::Homocysteine thiolactone is a product of an error-editing reaction, catalyzed by Escherichia coli and Saccharomyces cerevisiae methionyl-tRNA synthetases, which prevents incorporation of homocysteine into tRNA and protein both in vitro and in vivo. Here, homocysteine thiolactone is also shown to be synthesized by cult...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jakubowski H,Goldman E

    更新日期:1993-02-15 00:00:00

  • Staurosporine-related compounds, K252a and UCN-01, inhibit both cPKC and nPKC.

    abstract::The potent inhibitors of protein kinase C (PKC), H7, staurosporine, and staurosporine derivatives, were examined for their inhibitory effects on novel PKC (nPKC) isozymes delta and epsilon. H7 and staurosporine, usually used as selective inhibitors of PKC, showed similar inhibitory effects on cPKC (a mixture of cPKC a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mizuno K,Saido TC,Ohno S,Tamaoki T,Suzuki K

    更新日期:1993-09-13 00:00:00

  • The F-box protein FBXL18 promotes glioma progression by promoting K63-linked ubiquitination of Akt.

    abstract::F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Zhang J,Yang Z,Ou J,Xia X,Zhi F,Cui J

    更新日期:2017-01-01 00:00:00