Lack of adenosine deaminase deficiency in the mutant mouse wasted.

abstract：:Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the...

journal_title：FEBS letters

authors： Michel G,Rouillé Y,Chauvet MT,Chauvet J,Acher R

更新日期：1990-05-07 00:00:00

abstract：:Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-...

journal_title：FEBS letters

authors： Robins RJ,Bachmann P,Robinson T,Rhodes MJ,Yamada Y

更新日期：1991-11-04 00:00:00

abstract：:Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization....

journal_title：FEBS letters

authors： Nakanishi M,Takihara H,Minoru Y,Yagawa K

更新日期：1991-04-22 00:00:00

abstract：:Myasthenia gravis (MG) is caused by autoantibodies to the acetylcholine receptor (AChR). Experiments with fetal (alpha(2)betagammadelta) and adult (alpha(2)betaepsilondelta) AChR and with recombinant subunit dimers showed that some monoclonal antibodies (mAbs) against the main immunogenic region (MIR), located on the ...

journal_title：FEBS letters

authors： Fostieri E,Beeson D,Tzartos SJ

更新日期：2000-09-15 00:00:00

abstract：:The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant bl...

journal_title：FEBS letters

authors： Vauti F,Prochnow BR,Freese E,Ramasamy SK,Ruiz P,Arnold HH

更新日期：2007-12-11 00:00:00

abstract：:alpha-Dystroglycan was quantitatively enriched from mammalian brain based on its uniform reactivity with Vicia villosa agglutinin and resolved into sub-populations possessing or lacking the sulfated glucuronic acid epitope recognized by monoclonal antibody HNK-1. We generated a new monoclonal antibody specific for a g...

journal_title：FEBS letters

authors： McDearmon EL,Combs AC,Sekiguchi K,Fujiwara H,Ervasti JM

更新日期：2006-06-12 00:00:00

abstract：:In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...

journal_title：FEBS letters

authors： Costantini P,Chernyak BV,Petronilli V,Bernardi P

更新日期：1995-04-03 00:00:00

abstract：:We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...

journal_title：FEBS letters

authors： He C,Gu Q,Huang M,Xing J,Chen J

更新日期：2013-01-31 00:00:00

abstract：:MM-1 has been reported to repress the E-box-dependent transcription activity of c-Myc by recruiting histone deacetylase 1 complex via TIF1beta/KAP1. In this study, to identify target genes for c-Myc-MM-1-TIF1beta, we established rat-1 cells harboring the dominant-negative form of TIF1beta to abrogate the pathway from ...

journal_title：FEBS letters

authors： Satou A,Hagio Y,Taira T,Iguchi-Ariga SM,Ariga H

更新日期：2004-08-13 00:00:00

abstract：:In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...

journal_title：FEBS letters

authors： Sobue K,Kanda K,Kakiuchi S

更新日期：1982-12-13 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii were used to determine the role of two energy-dissipating systems, i.e., a free fatty acid (FFA)-activated, purine nucleotide-inhibited uncoupling protein (AcUCP) and a FFA-insensitive, purine nucleotide-activated ubiquinol alternative oxidase (AcAOX), in decreasing reac...

journal_title：FEBS letters

authors： Czarna M,Jarmuszkiewicz W

更新日期：2005-06-06 00:00:00

abstract：:Plant Golgi bodies possess unique morphological and functional characteristics that are key to several biological and biotechnological processes, such as transport of the cell's building blocks to energy-rich compartments, including chloroplasts, storage vacuoles and a cellulosic cell wall. During the last decade it h...

journal_title：FEBS letters

authors： Faso C,Boulaflous A,Brandizzi F

更新日期：2009-12-03 00:00:00

abstract：:The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

journal_title：FEBS letters

authors： Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

更新日期：1995-09-04 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...

journal_title：FEBS letters

authors： Satoh T,Sumiyoshi A,Yagi-Utsumi M,Sakata E,Sasakawa H,Kurimoto E,Yamaguchi Y,Li W,Joazeiro CA,Hirokawa T,Kato K

更新日期：2014-11-28 00:00:00

abstract：:The geometries are reported for interacting arginine-carboxyl pairs obtained from 37 high resolution protein structures solved to a resolution of 2.0 A or better. The closest interatomic distance between the guanidinium and carboxyl is less than 4.2 A for 74 arginine and carboxyl groups, with the majority of these lyi...

journal_title：FEBS letters

authors： Singh J,Thornton JM,Snarey M,Campbell SF

更新日期：1987-11-16 00:00:00

abstract：:Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...

journal_title：FEBS letters

authors： Huang J,Xu LG,Liu T,Zhai Z,Shu HB

更新日期：2006-02-06 00:00:00

abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...

journal_title：FEBS letters

authors： Agrotis A,Condron M,Bobik A

更新日期：2000-02-04 00:00:00

abstract：:Bovine alpha 2-antiplasmin (alpha 2AP) has been purified and partially characterized. The amino acid composition is very similar to that of human alpha 2AP, and the N-terminal (23 residues determined) and reactive site loop sequences (42 residues determined) are highly homologous to those of the human protein. Compare...

journal_title：FEBS letters

authors： Christensen S,Sottrup-Jensen L

更新日期：1992-11-02 00:00:00

abstract：:Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...

journal_title：FEBS letters

authors： Ohsawa S,Miura M

更新日期：2006-10-30 00:00:00

abstract：:Intravital multiphoton imaging allows visualization of infections and pathogenic mechanisms within intact organs in their physiological context. Today, most organs of mice and rats are applicable to in vivo or ex vivo imaging, opening completely new avenues for many researchers. Advances in fluorescent labeling of pat...

journal_title：FEBS letters

authors： Stolp B,Melican K

更新日期：2016-07-01 00:00:00

abstract：:We describe the use of a phage-displayed random pentadecamer peptide library for searching glycosphingolipid mimicking peptides. Two phage clones (AD-1 and AD-2) were selected by biopanning using monoclonal antibody AD117m, directed to lactotetraosylceramide (Lc4Cer). The amino acid sequences of the selected clones sh...

journal_title：FEBS letters

authors： Taki T,Ishikawa D,Hamasaki H,Handa S

更新日期：1997-11-24 00:00:00

abstract：:Intrinsically unstructured proteins (IUPs) are common in various proteomes and occupy a unique structural and functional niche in which function is directly linked to structural disorder. The evidence that these proteins exist without a well-defined folded structure in vitro is compelling, and justifies considering th...

journal_title：FEBS letters

authors： Tompa P

更新日期：2005-06-13 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:Vacuolar tandem-pore channels could not be analysed in Xenopus oocytes so far, due to misguided translocation. Owing to the conservation of their pore regions, we were able to prepare functional pore-chimeras between the plasma membrane localised TPK4 and vacuolar TPKs. Thereby, we found evidence that TPK2, TPK3 and T...

journal_title：FEBS letters

authors： Marcel D,Müller T,Hedrich R,Geiger D

更新日期：2010-06-03 00:00:00

abstract：:Myogenin, a member of the MyoD family which governs skeletal muscle differentiation, was identified as a pair of phosphorylated bands on SDS-PAGE during myogenesis. The slow migrating form was found to be hyperphosphorylated myogenin. In vitro phosphorylation by CDC2 kinase caused a prominent reduction in electrophore...

journal_title：FEBS letters

authors： Hashimoto N,Ogashiwa M,Okumura E,Endo T,Iwashita S,Kishimoto T

更新日期：1994-09-26 00:00:00

abstract：:Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

journal_title：FEBS letters

authors： Day NJ,Ellenbecker M,Voronina E

更新日期：2018-11-01 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...

journal_title：FEBS letters

authors： Carri MT,Galiazzo F,Ciriolo MR,Rotilio G

更新日期：1991-01-28 00:00:00

abstract：:The farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily that is primarily expressed in the enterohepatic system where it functions as intracellular sensor for bile acids. Ligand dependent FXR activation induces transcriptional responses to coordinately regulate bile acid, cholesterol, triglyceri...

journal_title：FEBS letters

authors： Modica S,Moschetta A

更新日期：2006-10-09 00:00:00