Abstract:
:We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scattered inside the cytoplasm and the cell nucleus. Pores are observed on canola protoplast cell membranes and cell walls when centrifuged with microbubbles, while the membrane of cells centrifuged alone remain intact and smooth. We hypothesize that the collision between the microbubbles and cells or the bursting of microbubbles are the main reasons for the formation of these pores. Biomaterials can diffuse into the cells once the pathway is created.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
He C,Gu Q,Huang M,Xing J,Chen Jdoi
10.1016/j.febslet.2012.12.005subject
Has Abstractpub_date
2013-01-31 00:00:00pages
285-90issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00910-6journal_volume
587pub_type
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