High levels of rhodopsin phosphorylation in missense mutations of C-terminal region of rhodopsin.

abstract：:Using oligonucleotide primers derived from the vesicular monoamine transporters sequences, a cDNA predicted to encode the bovine chromaffin granule amine transporter has been cloned (b-VMAT2). Surprisingly, its structure is more similar to the rat brain transporter (VMAT2), than to the rat adrenal counterpart (VMAT1)....

journal_title：FEBS letters

authors： Howell M,Shirvan A,Stern-Bach Y,Steiner-Mordoch S,Strasser JE,Dean GE,Schuldiner S

更新日期：1994-01-24 00:00:00

abstract：:Raman spectra of active Na+,K+-ATPase from pig kidney and membrane-bound products of its two-stage trypsinolysis, including alpha-subunit hydrophobic regions as well as the intact beta-subunit and hydrophobic regions of alpha- and beta-subunits, were measured to calculate the secondary structure of hydrophilic and hyd...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Arystarkhova EA,Arzamazova NM,Dzhandzhugazyan KN,Efremov RG,Nabiev IR,Modyanov NN

更新日期：1988-01-25 00:00:00

abstract：:Biological systems, from macromolecules to whole organisms, are robust if they continue to function, survive, or reproduce when faced with mutations, environmental change, and internal noise. I focus here on biological systems that are robust to mutations and ask whether such systems are more or less evolvable, in the...

journal_title：FEBS letters

authors： Wagner A

更新日期：2005-03-21 00:00:00

abstract：:Replication protein A (RP-A) was isolated from tobacco suspension cells and purified to near homogeneity by a procedure involving isolation of protoplasts, preparation of nuclei, nuclear lysis, binding to a column of single-stranded (ss) DNA cellulose and elution at different salt concentrations. The purified protein ...

journal_title：FEBS letters

authors： Garcia-Maya MM,Buck KW

更新日期：1997-08-11 00:00:00

abstract：:We show here that the genome of F9 teratocarcinoma cells growing in culture is heavily methylated but undergoes massive demethylation in tumors derived by subcutaneous injection of the cells. This demethylation occurs primarily in single copy gene sequences. As a result imprinted genes acquire their characteristic mon...

journal_title：FEBS letters

authors： Yeivin A,Levine A,Razin A

更新日期：1996-10-14 00:00:00

abstract：:Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...

journal_title：FEBS letters

authors： Nelson CH,Peng CC,Lutz JD,Yeung CK,Zelter A,Isoherranen N

更新日期：2016-08-01 00:00:00

abstract：:Here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical VlsE and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. The structural content of flavodoxin and VlsE is enhanced by 33% and 70%, respectively, in ...

journal_title：FEBS letters

authors： Perham M,Stagg L,Wittung-Stafshede P

更新日期：2007-10-30 00:00:00

abstract：:The concept of critical period plasticity in rat visual cortex has been studied in terms of changes in the level of filamentous actin and of the 200 kDa neurofilament polypeptide. Our results suggest that the postnatal developmental profile of filamentous actin is affected by visual experience, as a consequence of eye...

journal_title：FEBS letters

authors： Nona SN,Trowell SC,Cronly-Dillon JR

更新日期：1985-07-01 00:00:00

abstract：:The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

journal_title：FEBS letters

authors： Baldwin GS

更新日期：1995-02-06 00:00:00

abstract：:Transfer RNAs (tRNAs) are ancient macromolecules that have evolved under various environmental pressures as adaptors in translation in all forms of life but also towards alternative structures and functions. The present knowledge on both "canonical" and "deviating" signature motifs retrieved from vertical and horizont...

journal_title：FEBS letters

authors： Pütz J,Giegé R,Florentz C

更新日期：2010-01-21 00:00:00

abstract：:The mediator complex in the RNA polymerase II holoenzyme is known to be involved in transcriptional activation. The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date. While the expression of Srb7p under the control of a repre...

journal_title：FEBS letters

authors： Gromöller A,Lehming N

更新日期：2000-10-27 00:00:00

abstract：:Several deletion mutants of Raf-1 were expressed with v-ras p21 or 14-3-3 protein in COS-7 cells and Sf9 cells and the interaction of Raf-1 with ras p21 or with 14-3-3 protein in intact cells was examined. Raf(1-135) (residues 1-135) and Raf(1-322) interacted with v-ras p21, but other deletion mutants such as Raf(136-...

journal_title：FEBS letters

authors： Koyama S,Williams LT,Kikuchi A

更新日期：1995-07-17 00:00:00

abstract：:The involvement of Ca(2+) in the response to high Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), and Hg(2+) was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca(2+) when exposed to Cd(2+), and to a lesser extent to Cu(2+), but not to Mn(2+), Co(2+), Ni(2+), ...

journal_title：FEBS letters

authors： Ruta LL,Popa VC,Nicolau I,Danet AF,Iordache V,Neagoe AD,Farcasanu IC

更新日期：2014-08-25 00:00:00

abstract：:Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/auto...

journal_title：FEBS letters

authors： Dahiya Y,Pandey RK,Bhatt KH,Sodhi A

更新日期：2010-10-08 00:00:00

abstract：:Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here...

journal_title：FEBS letters

authors： Qiu F,Wang J,Spray DC,Scemes E,Dahl G

更新日期：2011-11-04 00:00:00

abstract：:While negative effect of smoking on the resistance to viral infections was known, the underlying mechanisms remained unclear. Here we report that products of cigarette smoking compromise the cellular anti-viral defenses by inhibiting the signaling induced by Type I interferon (IFN). Cigarette smoking condensate (but n...

journal_title：FEBS letters

authors： HuangFu WC,Liu J,Harty RN,Fuchs SY

更新日期：2008-09-22 00:00:00

abstract：:Crigler-Najjar (CN) disease is caused by a deficiency of the hepatic enzyme, bilirubin UDP-glucuronosyltransferase (B-UGT). We have found two CN type II patients, who were homozygous for a leucine to arginine transition at position 15 of B-UGT1. This mutation is expected to disrupt the hydrophobic core of the signal p...

journal_title：FEBS letters

authors： Seppen J,Steenken E,Lindhout D,Bosma PJ,Elferink RP

更新日期：1996-07-29 00:00:00

abstract：:Scorpion toxins have been found lacking effect on Na(+) current of its own sodium channel, whereas the molecular mechanism remains mystery. In this study, the binding affinity of pharmacologically distinct scorpion toxins was found much weaker to scorpion (Buthus martensii) nerve synaptosomes than to spider (Ornithoct...

journal_title：FEBS letters

authors： Zuo XP,He HQ,He M,Liu ZR,Xu Q,Ye JG,Ji YH

更新日期：2006-08-07 00:00:00

abstract：:Iron dependent regulator, IdeR, regulates the expression of genes in response to intracellular iron levels in M. tuberculosis. Orthologs of IdeR are present in all the sequenced genomes of mycobacteria. We have used a computational approach to identify conserved IdeR regulated genes across the mycobacteria and the gen...

journal_title：FEBS letters

authors： Yellaboina S,Ranjan S,Vindal V,Ranjan A

更新日期：2006-05-15 00:00:00

abstract：:In response to Ca(2+) signaling, cytosolic phospholipase A(2)alpha (cPLA(2)alpha) translocates from the cytosol to the perinuclear membrane, where downstream eicosanoid-synthetic enzymes, such as cyclooxygenase (COX), are localized. Although the spatiotemporal perinuclear colocalization of cPLA(2)alpha and COXs has be...

journal_title：FEBS letters

authors： Murakami M,Das S,Kim YJ,Cho W,Kudo I

更新日期：2003-07-10 00:00:00

abstract：:Hepatitis C virus (HCV) often establishes a persistent infection that leads to chronic liver diseases. The viral core protein modulates various cellular activities involved in this process. We found two mutations, K23E and V31A, in the core gene of the transfected HCV JFH-1 genome, which had been replicated for a prol...

journal_title：FEBS letters

authors： Kang JI,Kwon YC,Ahn BY

更新日期：2012-05-07 00:00:00

abstract：:A catalytic sequence motif PDX10-30(E/D)XK is found in many restriction enzymes. On the basis of sequence similarities and mapping of the conserved residues to the crystal structure of NgoMIV we suggest that residues D160, K182, R186, R188 and E195 contribute to the catalytic/DNA binding site of the Ecl18kI restrictio...

journal_title：FEBS letters

authors： Tamulaitis G,Solonin AS,Siksnys V

更新日期：2002-05-08 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:Silver and gold compounds were tested as potential inhibitors of aquaporins of plant- and human origin. Silver as AgNO(3) or silver sulfadiazine inhibited with high potency (EC(50) 1-10 microM) the water permeability of the peribacteroid membrane from soybean (containing Nodulin 26), the water permeability of plasma m...

journal_title：FEBS letters

authors： Niemietz CM,Tyerman SD

更新日期：2002-11-20 00:00:00

abstract：:We have studied the endosome-lysosome transfer of internalized epidermal growth factor receptor (EGFR) complexes in a cell-free system from rat liver. Analytical subfractionation of a postmitochondrial supernatant fraction showed that a pulse of internalized [(125)I]EGF was largely associated with a light endosomal fr...

journal_title：FEBS letters

authors： Authier F,Chauvet G

更新日期：1999-11-12 00:00:00

abstract：:The temperate phage phi H of the extremely halophilic archaebacterium Halobacterium salinarium encodes a repressor, Rep, which in the immune state represses the production of an early lytic transcript, denoted T4. Rep acts at the transcriptional level by blocking the promoter for T4. The promoter for the rep gene itse...

journal_title：FEBS letters

authors： Stolt P,Zillig W

更新日期：1994-05-16 00:00:00

abstract：:Persistent tumour necrosis factor alpha (TNF-alpha) exposure uncouples proximal T-cell receptor (TCR)-signalling events. Here, we demonstrate that chronic TNF-alpha exposure also attenuates signalling distal to the TCR, by specifically inhibiting Ca2+ influx evoked by thapsigargin in CD4+ T-cells. Mitogen-induced Ca2+...

journal_title：FEBS letters

authors： Church LD,Goodall JE,Rider DA,Bacon PA,Young SP

更新日期：2005-02-28 00:00:00

abstract：:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...

journal_title：FEBS letters

authors： Rafael J,Nicholls DG

更新日期：1984-05-07 00:00:00

abstract：:Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...

journal_title：FEBS letters

authors： Fischer HG,Eder C

更新日期：1995-10-09 00:00:00

abstract：:We have raised monoclonal antibodies (Mab) to the Mr 55,000 desmin polypeptide, electrophoretically purified from cytoskeletal preparations of isolated bovine heart Purkinje fibers. One of the Mabs, 39AB6, revealed desmin only in cow Purkinje fibers and did not react with desmins from other muscle cells, including ven...

journal_title：FEBS letters

authors： Virtanen I,Närvänen O,Thornell LE

更新日期：1990-07-02 00:00:00