Abstract:
:Scorpion toxins have been found lacking effect on Na(+) current of its own sodium channel, whereas the molecular mechanism remains mystery. In this study, the binding affinity of pharmacologically distinct scorpion toxins was found much weaker to scorpion (Buthus martensii) nerve synaptosomes than to spider (Ornithoctonus huwena) ones. The sodium channel cDNA from these two species were further cloned. The deduced proteins contain 1871 and 1987 amino acids respectively. Several key amino acid substitutions, i.e., A1610V, I1611L and S1617K, are found in IVS3-S4 constituting receptor site-3, and for receptor site-4, two residues (Leu-Pro) are inserted near IIS4 of scorpion sodium channel.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zuo XP,He HQ,He M,Liu ZR,Xu Q,Ye JG,Ji YHdoi
10.1016/j.febslet.2006.07.024subject
Has Abstractpub_date
2006-08-07 00:00:00pages
4508-14issue
18eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00859-3journal_volume
580pub_type
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