Abstract:
:Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts synergistically with growth factors possessing tyrosine kinase receptors on DNA synthesis. Thrombin induces synthesis of proteins of the fibrinolytic system and cell proliferation if it is present for at least 8 h. TRAP alone does not stimulate protein synthesis and is not mitogenic. However, in the presence of the aminopeptidase inhibitor amastatin all long-term effects of thrombin can be fully mimicked by TRAP. In conclusion, different effects of thrombin and TRAP may be related to the degradation of TRAP by cellular ectoenzymes. The recently cloned thrombin receptor accounts for early intracellular signals and long-term cellular effects that require sustained activation of this receptor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zacharias U,He CJ,Nguyen G,Sraer JD,Rondeau Edoi
10.1016/0014-5793(93)81716-dsubject
Has Abstractpub_date
1993-11-15 00:00:00pages
225-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(93)81716-Djournal_volume
334pub_type
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