Abstract:
:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min after the field pulse application into a balanced salt solution for 30 min at 37 degrees C.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vienken J,Zimmermann Udoi
10.1016/0014-5793(85)80315-xsubject
Has Abstractpub_date
1985-03-25 00:00:00pages
278-80issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)80315-Xjournal_volume
182pub_type
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