Effects of ciglitazone on insulin resistance and thermogenic responsiveness to acute cold in brown adipose tissue of genetically obese (ob/ob) mice.

abstract：:The effects of synthetic rat adrenomedullin (rAM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma, on receptor binding and cAMP generation were studied in cultured rat vascular smooth muscle cells (VSMC). A binding study using [125I]rAM revealed the presence of a single class of high-affi...

journal_title：FEBS letters

authors： Eguchi S,Hirata Y,Kano H,Sato K,Watanabe Y,Watanabe TX,Nakajima K,Sakakibara S,Marumo F

更新日期：1994-03-07 00:00:00

abstract：:U46619 is a potent platelet agonist, its binding to the thromboxane A2 receptor resulting in Gq-binding protein-mediated responses; nevertheless, it is unable to cause platelet aggregation, unless released ADP is present. In this study we demonstrate that Gi activation is the step U46619 lacks to cause platelet aggreg...

journal_title：FEBS letters

authors： Pulcinelli FM,Pesciotti M,Pignatelli P,Riondino S,Gazzaniga PP

更新日期：1998-09-11 00:00:00

abstract：:A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated p...

journal_title：FEBS letters

authors： Tsuchihara K,Wazawa T,Ishii Y,Yanagida T,Nishida R,Zheng XG,Ishiguro M,Yoshihara K,Hisatomi O,Tokunaga F

更新日期：2009-01-22 00:00:00

abstract：:10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

journal_title：FEBS letters

authors： Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

更新日期：1996-12-16 00:00:00

abstract：:Acylphosphatase (AcP) activity in prokaryotes was classically attributed to some aspecific acid phosphatases. We identified an open reading frame for a putative AcP in the b0968 Escherichia coli gene and purified the recombinant enzyme after checking by RT-PCR that it was indeed expressed. EcoAcP has a predicted typic...

journal_title：FEBS letters

authors： Ramazzotti M,Parrini C,Stefani M,Manao G,Degl'Innocenti D

更新日期：2006-12-22 00:00:00

abstract：:Curcumin, the active ingredient of the rhizome of Curcuma longa has anti-inflammatory, antioxidant and antiproliferative activities. Although its precise mode of action remains elusive, studies have shown that chemopreventive action of curcumin might be due to its ability to induce apoptosis in cancer cells. Curcumin ...

journal_title：FEBS letters

authors： Bhaumik S,Anjum R,Rangaraj N,Pardhasaradhi BV,Khar A

更新日期：1999-08-06 00:00:00

abstract：:The pl6INK4a tumor suppressor negatively regulates progression through the G1 phase of the mammalian cell cycle. To mimic the downmodulation of p16INK4a commonly seen in cancer, we designed and characterized a hammerhead ribozyme against exon E1alpha of the murine pl6INK4a transcript. Stable expression of the ribozyme...

journal_title：FEBS letters

authors： Nylandsted J,Rohde M,Bartek J,Strauss M

更新日期：1998-09-25 00:00:00

abstract：:The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...

journal_title：FEBS letters

authors： Ohnishi S,Güntert P,Koshiba S,Tomizawa T,Akasaka R,Tochio N,Sato M,Inoue M,Harada T,Watanabe S,Tanaka A,Shirouzu M,Kigawa T,Yokoyama S

更新日期：2007-02-06 00:00:00

abstract：:Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV

更新日期：1993-03-29 00:00:00

abstract：:Regulatory protein kinases are involved in various cellular processes such as proliferation, differentiation, and apoptosis. Using cDNA differential display, we identified MOK, a member of the mitogen-activated protein kinase superfamily, as one of the genes induced by a caudal-related homeobox transcription factor, C...

journal_title：FEBS letters

authors： Uesaka T,Kageyama N

更新日期：2004-08-27 00:00:00

abstract：:Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares...

journal_title：FEBS letters

authors： Baumann F,Milisav I,Neupert W,Herrmann JM

更新日期：2000-12-29 00:00:00

abstract：:Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...

journal_title：FEBS letters

authors： Shin EY,Min DS,Shin JC,Shin KS,Hyun MS,Ha KS,Kim HS,Ahn HY,Kim EG

更新日期：2001-11-16 00:00:00

abstract：:Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...

journal_title：FEBS letters

authors： Ying Y,Li L,Cao W,Yan D,Zeng Q,Kong X,Lu L,Yan M,Xu X,Qu J,Su Q,Ma X

更新日期：2012-10-19 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase r...

journal_title：FEBS letters

authors： Banas B,Eberle J,Banas B,Schlöndorff D,Luckow B

更新日期：2001-12-07 00:00:00

abstract：:A 15-ns molecular dynamics simulation of the fully hydrated liquid-crystalline dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer containing approximately 22 mol% Chol was carried out. The generated trajectory was analysed to investigate the mechanism of the Chol condensing effect on DMPC hydrocarbon chain...

journal_title：FEBS letters

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-07-27 00:00:00

abstract：:The interaction of the adrenoleukodystrophy protein (ALDP), mutated in the peroxisomal disorder X-linked adrenoleukodystrophy, and the very long-chain acyl-CoA synthetase (VLACS), the enzyme whose function is missing in this disease, remains obscure. As a first step to studying this interaction in wild type versus ALD...

journal_title：FEBS letters

authors： Berger J,Truppe C,Neumann H,Forss-Petter S

更新日期：1998-03-27 00:00:00

abstract：:The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is...

journal_title：FEBS letters

authors： Korolkov SN,Garnovskaya MN,Basov AS,Chunaev AS,Dumler IL

更新日期：1990-09-17 00:00:00

abstract：:The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dyn...

journal_title：FEBS letters

authors： Vicker MG

更新日期：2002-01-02 00:00:00

abstract：:Locus control regions (LCRs) are cis-acting elements that confer position-independent and copy-number-dependent expression upon associated genes in transgenic mice. Here we show the second example of the use of an LCR (the rat LAP LCR) in a stable expression vector system, used here in conjunction with the rat liver (...

journal_title：FEBS letters

authors： Shelton PA,Dart C

更新日期：1996-08-12 00:00:00

abstract：:Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factors VIIa and Xa through Kunitz domains, thereby inhibiting the activity of tissue factor. However, it has been shown that the C-terminal of this inhibitor is essential for the maximal anticoagulant activity of TFPI. We have investigated th...

journal_title：FEBS letters

authors： Ettelaie C,Adam JM,James NJ,Oke AO,Harrison JA,Bunce TD,Bruckdorfer KR

更新日期：1999-12-17 00:00:00

abstract：:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

journal_title：FEBS letters

authors： Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

更新日期：2000-04-14 00:00:00

abstract：:The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated th...

journal_title：FEBS letters

authors： Kanda D,Takagi H,Toyoda M,Horiguchi N,Nakajima H,Otsuka T,Mori M

更新日期：2002-05-22 00:00:00

abstract：:The A-rich bulge in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutants have a reduced maxim...

journal_title：FEBS letters

authors： Pace U,Szostak JW

更新日期：1991-03-11 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic recepto...

journal_title：FEBS letters

authors： Hurst RS,Zhu X,Boulay G,Birnbaumer L,Stefani E

更新日期：1998-02-06 00:00:00

abstract：:A high-throughput cell-free protein synthesis method has been described. The methodology is based on a bilayer diffusion system that enables the continuous supply of substrates, together with the continuous removal of small byproducts, through a phase between the translation mixture and substrate mixture. With the use...

journal_title：FEBS letters

authors： Sawasaki T,Hasegawa Y,Tsuchimochi M,Kamura N,Ogasawara T,Kuroita T,Endo Y

更新日期：2002-03-06 00:00:00

abstract：:Retroviral proteinases (PRs) are essential for retrovirus infectivity but the mechanism of their activity regulation is poorly understood. We investigated possible involvement in this process of the C-terminal domain (CTD) of betaretroviral PRs. We found that the presence of CTD attenuates proteolytic activity of Maso...

journal_title：FEBS letters

authors： Svec M,Bauerová H,Pichová I,Konvalinka J,Strísovský K

更新日期：2004-10-08 00:00:00

abstract：:Heme is degraded by heme oxygenase to form iron, carbon monoxide (CO), and biliverdin. However, information about the catabolism of heme in erythroid cells is limited. In this study, we showed the production and export of bilirubin in murine erythroleukemia (MEL) cells. The production of bilirubin by MEL cells was enh...

journal_title：FEBS letters

authors： Mu A,Li M,Tanaka M,Adachi Y,Tai TT,Liem PH,Izawa S,Furuyama K,Taketani S

更新日期：2016-05-01 00:00:00

abstract：:The Ca2+ receptor mechanism of the parathyroid cell was studied using La3+ as a probe. La3+ was found to bind to the cell surface without further penetration. Measurements of 45Ca fluxes and the cytoplasmic Ca2+ concentration (Ca2+i) revealed a stimulatory component in the action of La3+ on Ca2+ permeability resulting...

journal_title：FEBS letters

authors： Gylfe E,Larsson R,Johansson H,Nygren P,Rastad J,Wallfelt C,Akerström G

更新日期：1986-09-01 00:00:00