A bilayer cell-free protein synthesis system for high-throughput screening of gene products.

abstract：:Nuclear proteins are actively and posttranslationally transported across the nuclear envelope. This transport is a highly selective process that can be divided into two steps, receptor-binding followed by translocation through the nuclear envelope. Receptor-binding is mediated by nuclear localization signals that have...

journal_title：FEBS letters

authors： Wagner P,Kunz J,Koller A,Hall MN

更新日期：1990-11-26 00:00:00

abstract：:Lysophospholipids are liberated during venomous action. In this study we demonstrated that lysophosphatidyl choline (LPC) of various acyl chains enhances considerably the activity of snake venom phosphodiesterase (PDE). Lysophosphatidic acid (LPA) and its cyclic form (cLPA), on the other hand, were found to inhibit th...

journal_title：FEBS letters

authors： Mamillapalli R,Haimovitz R,Ohad M,Shinitzky M

更新日期：1998-10-02 00:00:00

abstract：:A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pr...

journal_title：FEBS letters

authors： Minakuchi K,Yabushita T,Masumura T,Ichihara K,Tanaka K

更新日期：1994-01-10 00:00:00

abstract：:We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

journal_title：FEBS letters

authors： Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

更新日期：1999-05-07 00:00:00

abstract：:Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...

journal_title：FEBS letters

authors： Rastogi R,Verma JK,Singh V,Krishnamurthy G,Sood C,Kapoor A,Kumar K,Ansari I,Mukhopadhyay A

更新日期：2020-12-14 00:00:00

abstract：:The crystal structure at pH 4 of the complex of glucoamylase II(471) from Aspergillus awamori var. X100 with the pseudotetrasaccharide D-gluco-dihydroacarbose has been refined to an R-factor of 0.125 against data to 2.2 A resolution. The first two residues of the inhibitor bind at a position nearly identical to those ...

journal_title：FEBS letters

authors： Stoffer B,Aleshin AE,Firsov LM,Svensson B,Honzatko RB

更新日期：1995-01-16 00:00:00

abstract：:We investigated transgenic tobacco lines which express different amounts of the barley JIP 23. In these plants the amount of several proteins decreased proportionally to increasing amounts of JIP 23 whereas the transcript levels were constant as determined for the small and the large subunit of RuBPCase. However, the ...

journal_title：FEBS letters

authors： Görschen E,Dunaeva M,Reeh I,Wasternack C

更新日期：1997-12-08 00:00:00

abstract：:Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

journal_title：FEBS letters

authors： Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

更新日期：2017-09-01 00:00:00

abstract：:The residues on MAP kinase kinase-1 (MAPKK1) phosphorylated by MAP kinase in vitro have been identified as Thr-291 and Thr-385. Both threonines are phosphorylated in PC12 cells and the 32P-labelling of each residue increases after stimulation with nerve growth factor (NGF). The results establish that MAPKK1 is a physi...

journal_title：FEBS letters

authors： Saito Y,Gomez N,Campbell DG,Ashworth A,Marshall CJ,Cohen P

更新日期：1994-03-14 00:00:00

abstract：:Domain structures of porcine lung and testicular angiotensin-converting enzymes (ACE) were studied by radiation inactivation to test the hypothesis that lung ACE has two catalytic sites localized to discrete, structurally independent domains (the N- and C-domains) of approximately equal size. The minimum functional si...

journal_title：FEBS letters

authors： Sakaguchi H,Hirose S,Kume T,Hagiwara H

更新日期：1992-06-29 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, c...

journal_title：FEBS letters

authors： Miyato Y,Ibuki Y,Ohyama H,Yamada T,Goto R

更新日期：2001-08-24 00:00:00

abstract：:Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were d...

journal_title：FEBS letters

authors： Hou SC,Chan LW,Chou YC,Su CY,Chen X,Shih YL,Tsai PC,Shen CK,Yan YT

更新日期：2009-09-03 00:00:00

abstract：:In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...

journal_title：FEBS letters

authors： Ordiz I,Herrero P,Rodicio R,Moreno F

更新日期：1995-07-03 00:00:00

abstract：:The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 5...

journal_title：FEBS letters

authors： Petrovskaya LE,Lukashev EP,Chupin VV,Sychev SV,Lyukmanova EN,Kryukova EA,Ziganshin RH,Spirina EV,Rivkina EM,Khatypov RA,Erokhina LG,Gilichinsky DA,Shuvalov VA,Kirpichnikov MP

更新日期：2010-10-08 00:00:00

abstract：:The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...

journal_title：FEBS letters

authors： Hakoshima T

更新日期：2018-02-01 00:00:00

abstract：:The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains thre...

journal_title：FEBS letters

authors： Gaston K,Fried M

更新日期：1994-06-27 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreem...

journal_title：FEBS letters

authors： Gesierich U,Pfeil W

更新日期：1996-09-16 00:00:00

abstract：:Mutations in retinol dehydrogenase 12 (RDH12) cause severe retinal degeneration. However, some of the disease-associated RDH12 mutants retain significant catalytic activity, indicating the existence of additional pathophysiological mechanisms. This study demonstrates that the catalytically active T49M and I51N mutants...

journal_title：FEBS letters

authors： Lee SA,Belyaeva OV,Kedishvili NY

更新日期：2010-02-05 00:00:00

abstract：:Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lack...

journal_title：FEBS letters

authors： Abe Y,Goda W,Ikeshima-Kataoka H,Yasui M

更新日期：2017-12-01 00:00:00

abstract：:Tau phosphorylation plays a crucial role in microtubule stabilization and in Alzheimer's disease. To characterize the molecular mechanisms of tau binding on microtubules, we synthesized the peptide R1 (QTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQI), reproducing the first tau microtubule binding motif. We thermodynamically charac...

journal_title：FEBS letters

authors： Devred F,Douillard S,Briand C,Peyrot V

更新日期：2002-07-17 00:00:00

abstract：:Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...

journal_title：FEBS letters

authors： Ohsawa S,Miura M

更新日期：2006-10-30 00:00:00

abstract：:The thermal blockage of QA-. oxidation was analysed in PS II membrane fragments by monitoring flash-induced changes of the relative fluorescence quantum yield as a function of temperature. The results obtained reveal: (a) in dark-adapted samples the fraction of QA-. that is not reoxidised within a time domain of 10 s ...

journal_title：FEBS letters

authors： Reifarth F,Renger G

更新日期：1998-05-29 00:00:00

abstract：:The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elem...

journal_title：FEBS letters

authors： Lavrentieva I,Broude NE,Lebedev Y,Gottesman II,Lukyanov SA,Smith CL,Sverdlov ED

更新日期：1999-01-29 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...

journal_title：FEBS letters

authors： Stait SE,Leake DS

更新日期：1994-03-21 00:00:00

abstract：:Poly-ADP ribosylation of nuclear proteins is activated when poly(ADP-ribose) polymerase (PARP), a nuclear zinc-finger enzyme, binds to single-strand DNA breaks. To understand how the signal emerging from its DNA-binding domain (DBD) bound to such breaks is transduced to its catalytic domain, the structure-function rel...

journal_title：FEBS letters

authors： Trucco C,Flatter E,Fribourg S,de Murcia G,Ménissier-de Murcia J

更新日期：1996-12-16 00:00:00

abstract：:Apoptotic protease activating factor-1 (Apaf-1) is an adaptor molecule essential for caspase-9 activation. Subcellular analysis of Apaf-1 in NIH-3T3 fibroblasts and the immature murine B cell lymphoma WEHI-231 indicates that Apaf-1 is localized in the Golgi apparatus and cytoplasm. Bcl-2 overexpression in WEHI-231 cel...

journal_title：FEBS letters

authors： Ruiz-Vela A,Albar JP,Martínez CA

更新日期：2001-07-13 00:00:00

abstract：:A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pig...

journal_title：FEBS letters

authors： Völkel H,Beitz E,Klumpp S,Schultz JE

更新日期：1996-01-15 00:00:00