The conformational stability of alpha-crystallin is rather low: calorimetric results.

abstract：:The in vitro methylation of the +6, +24, and -215 located 5'-CCGG-3' sequences in the late E2A promoter of adenovirus type 2 (Ad2) DNA abrogates promoter function. 5-Methyldeoxycytidine (5-mC) at positions +6 and +24 in both or either of the two DNA complements in the late E2A promoter abolishes the formation of a hig...

journal_title：FEBS letters

authors： Hermann R,Doerfler W

更新日期：1991-04-09 00:00:00

abstract：:Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cros...

journal_title：FEBS letters

authors： Trifilieff E,Luu B,Nussbaum JL,Roussel G,Espinosa de los Monteros A,Sabatier JM,Van Rietschoten J

更新日期：1986-03-31 00:00:00

abstract：:Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...

journal_title：FEBS letters

authors： Kitagawa Y,Murakami A,Sugimoto E

更新日期：1984-01-02 00:00:00

abstract：:The role of tRNA in translating the genetic code has received considerable attention over the last 50 years, and we now know in great detail how particular amino acids are specifically selected and brought to the ribosome in response to the corresponding mRNA codon. Over the same period, it has also become increasingl...

journal_title：FEBS letters

authors： Banerjee R,Chen S,Dare K,Gilreath M,Praetorius-Ibba M,Raina M,Reynolds NM,Rogers T,Roy H,Yadavalli SS,Ibba M

更新日期：2010-01-21 00:00:00

abstract：:Moloney murine leukemia virus reverse transcriptase (RT) selectively uses deoxyribonucleotides over ribonucleotides (rNTPs) as substrates. Substitution of F155 with valine (F155V) was previously found to increase the enzyme's affinity for rNTPs, though without affecting the V(max) for catalysis, and thereby conferred ...

journal_title：FEBS letters

authors： Liu S,Goff SP,Gao G

更新日期：2006-02-20 00:00:00

abstract：:Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...

journal_title：FEBS letters

authors： Petersen TE

更新日期：1988-04-11 00:00:00

abstract：:Escherichia coli heat-shock proteins GroEL and GroES stimulate (in an ATP-dependent manner) the folding of various proteins. In this study scanning microcalorimetry was applied to investigate GroEL thermostability in the presence of its ligands. Mg2+ and K+ ions stabilize while ADP destabilizes the GroEL molecule agai...

journal_title：FEBS letters

authors： Surin AK,Kotova NV,Kashparov IA,Marchenkov VV,Marchenkova SYu,Semisotnov GV

更新日期：1997-04-01 00:00:00

abstract：:The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...

journal_title：FEBS letters

authors： Kotelnikova RA,Kotelnikov AI,Bogdanov GN,Romanova VS,Kuleshova EF,Parnes ZN,Vol'pin ME

更新日期：1996-07-01 00:00:00

abstract：:For good function, membrane lipids have to be arranged appropriately and be in the correct physical state. In poikilotherms, exposure to cold stress or heat shock can alter membrane properties such that, unless they are corrected quickly, damage and, possibly, death can result. Low temperature stress is countered by m...

journal_title：FEBS letters

authors： Guschina IA,Harwood JL

更新日期：2006-10-09 00:00:00

abstract：:The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

journal_title：FEBS letters

authors： Chiesi M,Schwaller R

更新日期：1989-02-13 00:00:00

abstract：:The tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and C...

journal_title：FEBS letters

authors： Charrin S,Manié S,Oualid M,Billard M,Boucheix C,Rubinstein E

更新日期：2002-04-10 00:00:00

abstract：:Lactase phlorizin hydrolase (LPH; EC 3.2.1.62) is a membrane-bound, family 1 beta-glycosidase found on the brush border of the mammalian small intestine. LPH, purified from sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone glycosides. The catalytic efficiency (k(cat)/K(m)) for the hy...

journal_title：FEBS letters

authors： Day AJ,Cañada FJ,Díaz JC,Kroon PA,Mclauchlan R,Faulds CB,Plumb GW,Morgan MR,Williamson G

更新日期：2000-02-25 00:00:00

abstract：:Phospholipase C (PLC) was purified from squid retina. Soluble Gq alpha, membrane Gq alpha and G beta gamma were isolated from GTP gamma S-treated and light-illuminated photoreceptor membranes. The membrane Gq alpha stimulated phosphatidyl inositol-phospholipase C (PI-PLC) activity in a dose-dependent manner. Soluble G...

journal_title：FEBS letters

authors： Suzuki T,Terakita A,Narita K,Nagai K,Tsukahara Y,Kito Y

更新日期：1995-12-27 00:00:00

abstract：:The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation...

journal_title：FEBS letters

authors： Millet O,Bernadó P,Garcia J,Rizo J,Pons M

更新日期：2002-04-10 00:00:00

abstract：:Avian red blood cells contain a millimolar amount of inositol polyphosphate which plays a role as an allosteric effector of hemoglobin. We confirmed the structure of this substance by NMR techniques as purely myo-inositol 1,3,4,5,6-pentakisphosphate. Based on present knowledge this effector is synthesized from inosito...

journal_title：FEBS letters

authors： Mayr GW,Dietrich W

更新日期：1987-03-23 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:(1-->3)-alpha-glucanases catalyze the hydrolysis of fungal cell wall (1-->3)-alpha-glucan, and function during cell division of yeasts containing this cell wall component or act in mycoparasitic processes. Here, we characterize the mechanism of action of the (1-->3)-alpha-glucanase MutAp from the mycoparasitic fungus ...

journal_title：FEBS letters

authors： Grün CH,Dekker N,Nieuwland AA,Klis FM,Kamerling JP,Vliegenthart JF,Hochstenbach F

更新日期：2006-07-10 00:00:00

abstract：:The hypothesis that specific protein kinase C (PKC) isoforms regulate dopamine transporter (DAT) function was tested in Xenopus laevis oocytes expressing human (h)DAT. Activation of conventional PKCs (cPKCs) and novel PKCs (nPKCs) using 10 nM phorbol 12-myristate 13-acetate (PMA) significantly inhibited DAT-associated...

journal_title：FEBS letters

authors： Doolen S,Zahniser NR

更新日期：2002-04-10 00:00:00

abstract：:The geometries are reported for interacting arginine-carboxyl pairs obtained from 37 high resolution protein structures solved to a resolution of 2.0 A or better. The closest interatomic distance between the guanidinium and carboxyl is less than 4.2 A for 74 arginine and carboxyl groups, with the majority of these lyi...

journal_title：FEBS letters

authors： Singh J,Thornton JM,Snarey M,Campbell SF

更新日期：1987-11-16 00:00:00

abstract：:Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecip...

journal_title：FEBS letters

authors： Peng X,Zhang Y,Zhang H,Graner S,Williams JF,Levitt ML,Lokshin A

更新日期：1999-03-05 00:00:00

abstract：:Growth of E. coli in the presence of the protonophorous uncoupler pentachlorophenol is shown to strongly enhance levels of cytochrome d, a putative Na(+)-motive oxidase. This effect was found to be arrested by chloramphenicol and stimulated by high Na+ concentration in the growth medium. The induction of cytochrome d ...

journal_title：FEBS letters

authors： Bogachev AV,Murtazina RA,Skulachev VP

更新日期：1993-12-20 00:00:00

abstract：:We identified cDNAs coding for homologues to tetrapod prion proteins (PrPs) in Atlantic salmon (Salmo salar) and Japanese pufferfish (Fugu rubripes), which were termed 'similar to PrPs' (stPrPs). Besides significant sequence homologies the fish stPrPs display characteristic structural features in common with tetrapod ...

journal_title：FEBS letters

authors： Oidtmann B,Simon D,Holtkamp N,Hoffmann R,Baier M

更新日期：2003-03-13 00:00:00

abstract：:The protein kinase D family of enzymes consists of three isoforms: PKD1/PKCmu PKD2 and PKD3/PKCnu. They all share a similar architecture with regulatory sub-domains that play specific roles in the activation, translocation and function of the enzymes. The PKD enzymes have recently been implicated in very diverse cellu...

journal_title：FEBS letters

authors： Rykx A,De Kimpe L,Mikhalap S,Vantus T,Seufferlein T,Vandenheede JR,Van Lint J

更新日期：2003-07-03 00:00:00

abstract：:Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...

journal_title：FEBS letters

authors： Choi HJ,Park JH,Park JH,Lee KB,Oh SM

更新日期：2015-12-21 00:00:00

abstract：:Insulin receptors (IRs) that are truncated at the end of the ectodomain form dimers that bind insulin with different characteristics to wild type receptors. These soluble IRs have lowered affinity for insulin compared with full-length IR, and exhibit linear Scatchard plots in contrast to the curvilinear plots obtained...

journal_title：FEBS letters

authors： Hoyne PA,Cosgrove LJ,McKern NM,Bentley JD,Ivancic N,Elleman TC,Ward CW

更新日期：2000-08-11 00:00:00

abstract：:Affinity labeling studies have identified several conserved tyrosine residues in the alpha subunit of the nicotinic acetylcholine receptor (alpha Y93, alpha Y190, and alpha Y198) as being in or near the ligand binding site. Mutagenesis studies from several laboratories have shown that substitution of phenylalanine for...

journal_title：FEBS letters

authors： Aylwin ML,White MM

更新日期：1994-07-25 00:00:00

abstract：:Expression from a plasmid of the complete gene, including the codons for the N-terminal periplasmic targeting signal, for cytochrome c550 of Paracoccus denitrificans led to the formation of the holo protein in the periplasms of both P. denitrificans and Escherichia coli. Expression of the gene from which the region co...

journal_title：FEBS letters

authors： Sambongi Y,Ferguson SJ

更新日期：1994-02-28 00:00:00

abstract：:Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...

journal_title：FEBS letters

authors： Esposito C,Carullo P,Pedone E,Graziano G,Del Vecchio P,Berisio R

更新日期：2010-03-19 00:00:00

abstract：:Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

journal_title：FEBS letters

authors： Grammenoudi S,Kosmidis S,Skoulakis EM

更新日期：2006-08-21 00:00:00

abstract：:Glycogen synthase kinase-5 (casein kinase-II) phosphorylates glycogen synthase on a serine termed site 5. This residue is just C-terminal to the 3 serines phosphorylated by glycogen synthase kinase-3, which are critical for the hormonal regulation of glycogen synthase in vivo. Although phosphorylation of site 5 does n...

journal_title：FEBS letters

authors： Picton C,Woodgett J,Hemmings B,Cohen P

更新日期：1982-12-13 00:00:00