Abstract:
:Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degraded slowly with a half-life of more than 16 min. Valinomycin, nonactin, A23187, X-537A (lasalocid), bromo-lasalocid, and carbonyl cyanide m-chlorophenylhydrazone did not exhibit these effects at concentrations at which they did not inhibit protein synthesis of the cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kitagawa Y,Murakami A,Sugimoto Edoi
10.1016/0014-5793(84)80029-0subject
Has Abstractpub_date
1984-01-02 00:00:00pages
133-7issue
1eissn
0014-5793issn
1873-3468journal_volume
165pub_type
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