Abstract:
:The hypothesis that specific protein kinase C (PKC) isoforms regulate dopamine transporter (DAT) function was tested in Xenopus laevis oocytes expressing human (h)DAT. Activation of conventional PKCs (cPKCs) and novel PKCs (nPKCs) using 10 nM phorbol 12-myristate 13-acetate (PMA) significantly inhibited DAT-associated transport currents. This effect was reversed by isoform-non-selective PKC inhibitors, selective inhibitors of cPKCs and deltaPKC, and by Ca2+ chelation. By contrast, the epsilonPKC translocation inhibitor peptide had no effect on PMA-induced inhibition of hDAT transport-associated currents. Thus, the primary mechanism by which PMA regulates hDAT expressed in oocytes appears to be by activating cPKC(s).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Doolen S,Zahniser NRdoi
10.1016/s0014-5793(02)02554-1keywords:
subject
Has Abstractpub_date
2002-04-10 00:00:00pages
187-90issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302025541journal_volume
516pub_type
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