MiRNA let-7g regulates skeletal myoblast motility via Pinch-2.

Abstract:

:Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RNA-binding protein IMP-2/Igf2bp2. We now show that the expression of Pinch-2 is also regulated by the miRNA let-7g. Let-7g and IMP-2 repress Pinch-2 expression independently of each other. A knock-down of let-7g leads to an increase in Pinch-2 expression, and to a decrease of cell motility, which can be reversed by a simultaneous knock-down of Pinch-2. We conclude that let-7g controls the motility of mouse myoblasts in cell culture by post-transcriptionally regulating the expression of Pinch-2.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Boudoukha S,Rivera Vargas T,Dang I,Kropp J,Cuvellier S,Gautreau A,Polesskaya A

doi

10.1016/j.febslet.2014.02.057

subject

Has Abstract

pub_date

2014-05-02 00:00:00

pages

1623-9

issue

9

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(14)00186-0

journal_volume

588

pub_type

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