Abstract:
:Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no evidence for a signal sequence. The gene had a low G + C base ratio which differs from the rest of the Pasteurella genome. There was no significant homology with other known proteins, although a motif found in certain bacterial toxins which are ADP-ribosyl transferases is present. A recombinant expressing only part of the PMT gene was not mitogenic.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lax AJ,Chanter N,Pullinger GD,Higgins T,Staddon JM,Rozengurt Edoi
10.1016/0014-5793(90)80809-wsubject
Has Abstractpub_date
1990-12-17 00:00:00pages
59-64issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)80809-Wjournal_volume
277pub_type
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