Thrombin-induced proliferation and expression of platelet-derived growth factor-A chain gene in human vascular smooth muscle cells.

abstract：:The half-lives of mRNAs in yeast vary from about 1 to over 100 min. While mRNA stabilities must strongly influence overall gene expression in this organism, very little is known about how they are determined. Labellings of yeast cells were conducted to investigate whether the 5'-cap structures of yeast mRNAs might inf...

journal_title：FEBS letters

authors： Piper PW,Curran B,Davies W,Hirst K,Seward K

更新日期：1987-08-10 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alph...

journal_title：FEBS letters

authors： García-Palomero E,Cuchillo-Ibáñez I,García AG,Renart J,Albillos A,Montiel C

更新日期：2000-09-22 00:00:00

abstract：:In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...

journal_title：FEBS letters

authors： Girdwood D,Robertson M,Gordon C

更新日期：2012-05-21 00:00:00

abstract：:The importance of the individual amino acid residues of AIP (KKALRRQEAVDAL), a highly specific inhibitor of calmodulin-dependent protein kinase II (CaMKII), was studied. Replacement of Arg6, Gln7, or Ala9 by other amino acid residues produced a marked increase in the IC50 value. Leu4 and Val10 were also sensitive to r...

journal_title：FEBS letters

authors： Ishida A,Shigeri Y,Tatsu Y,Uegaki K,Kameshita I,Okuno S,Kitani T,Yumoto N,Fujisawa H

更新日期：1998-05-01 00:00:00

abstract：:Stresses that impair the function of the endoplasmic reticulum (ER) lead to an accumulation of unfolded protein in the ER. Under these conditions, the expression of a variety of genes involved in preventing the accumulation of the unfolded proteins is induced. Yeast Hrd1p is an ER stress-inducible ER membrane protein ...

journal_title：FEBS letters

authors： Kaneko M,Ishiguro M,Niinuma Y,Uesugi M,Nomura Y

更新日期：2002-12-04 00:00:00

abstract：:Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...

journal_title：FEBS letters

authors： Marques SA,Loureiro AP,Gomes OF,Garcia CC,Di Mascio P,Medeiros MH

更新日期：2004-02-27 00:00:00

abstract：:Mammalian pancreatic phospholipase A2 (PLA2-I) has its specific receptor through which PLA2-I induces a variety of biological responses. In this study, a fundamental relationship between the enzymatic and the receptor-binding activities of PLA2-I was investigated. The specific binding of PLA2-I to the receptor was fou...

journal_title：FEBS letters

authors： Ishizaki J,Kishino J,Teraoka H,Ohara O,Arita H

更新日期：1993-06-21 00:00:00

abstract：:Formylmethanofuran dehydrogenase, a key enzyme of methanogenesis, was purified 100-fold from methanol grown Methanosarcina barkeri to apparent homogeneity and a specific activity of 34 mumol.min-1.mg protein-1. Molybdenum was found to co-migrate with the enzyme activity. The molybdenum content of purified preparations...

journal_title：FEBS letters

authors： Karrasch M,Börner G,Enssle M,Thauer RK

更新日期：1989-08-14 00:00:00

abstract：:In order to study the function of the bovine MAP4 microtubule-binding domain (the assembly-promoting (AP) sequence region), a fragment corresponding to the AP sequence region was prepared using an Escherichia coli expression system. When the fragment was mixed with purified tubulin at 37 degrees C, the fragment caused...

journal_title：FEBS letters

authors： Katsuki M,Tokuraku K,Nakagawa H,Murofushi H,Kotani S

更新日期：1997-11-24 00:00:00

abstract：:Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...

journal_title：FEBS letters

authors： Yoshimura S,Ikemura H,Watanabe H,Aimoto S,Shimonishi Y,Hara S,Takeda T,Miwatani T,Takeda Y

更新日期：1985-02-11 00:00:00

abstract：:Crigler-Najjar (CN) disease is caused by a deficiency of the hepatic enzyme, bilirubin UDP-glucuronosyltransferase (B-UGT). We have found two CN type II patients, who were homozygous for a leucine to arginine transition at position 15 of B-UGT1. This mutation is expected to disrupt the hydrophobic core of the signal p...

journal_title：FEBS letters

authors： Seppen J,Steenken E,Lindhout D,Bosma PJ,Elferink RP

更新日期：1996-07-29 00:00:00

abstract：:Protective antigen (PA) is the central receptor binding component of anthrax toxin, which translocates catalytic components of the toxin into the cytosol of mammalian cells. Ever since the crystal structure of PA was solved, there have been speculations regarding the possible role of calcium ions present in domain I o...

journal_title：FEBS letters

authors： Gupta PK,Chandra H,Gaur R,Kurupati RK,Chowdhury S,Tandon V,Singh Y,Maithal K

更新日期：2003-11-20 00:00:00

abstract：:Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...

journal_title：FEBS letters

authors： Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SG

更新日期：2016-02-01 00:00:00

abstract：:Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...

journal_title：FEBS letters

authors： Tanfin Z,Goureau O,Milligan G,Harbon S

更新日期：1991-01-14 00:00:00

abstract：:The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...

journal_title：FEBS letters

authors： Katayama K,Dobashi Y,Kitagawa M,Kamekura S,Kawai M,Kadoya Y,Kameya T

更新日期：2001-12-14 00:00:00

abstract：:A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

journal_title：FEBS letters

authors： Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

更新日期：2009-06-18 00:00:00

abstract：:Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts...

journal_title：FEBS letters

authors： Zacharias U,He CJ,Nguyen G,Sraer JD,Rondeau E

更新日期：1993-11-15 00:00:00

abstract：:Poly-ADP ribosylation of nuclear proteins is activated when poly(ADP-ribose) polymerase (PARP), a nuclear zinc-finger enzyme, binds to single-strand DNA breaks. To understand how the signal emerging from its DNA-binding domain (DBD) bound to such breaks is transduced to its catalytic domain, the structure-function rel...

journal_title：FEBS letters

authors： Trucco C,Flatter E,Fribourg S,de Murcia G,Ménissier-de Murcia J

更新日期：1996-12-16 00:00:00

abstract：:Prostaglandin F2alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase...

journal_title：FEBS letters

authors： Pettitt TR,Wakelam MJ

更新日期：1998-05-15 00:00:00

abstract：:Tight couple 70 S ribosomes are converted to loose couple ones on enzymatic binding of phenylalanyl-tRNA. Enzymatic binding at 0 degree C as well as nonenzymatic binding does not lead to any change. Further, no change takes place when the P site is occupied by N-acetylphenylalanyl-tRNA. Loose couple 70 S ribosomes are...

journal_title：FEBS letters

authors： Srivastava S,Burma DP

更新日期：1985-07-01 00:00:00

abstract：:Myeloma cells specifically localize in the bone marrow and rarely circulate in blood. To study whether this immobilization could be partially explained by the presence of constitutively activated integrins, particularly alpha4beta1, we used the activation reporter HUTS-21 anti-beta1 mAb. These analyses showed that bet...

journal_title：FEBS letters

authors： García-Gila M,Cabañas C,García-Pardo A

更新日期：1997-12-01 00:00:00

abstract：:Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...

journal_title：FEBS letters

authors： Ferraretto A,Gravaghi C,Fiorilli A,Tettamanti G

更新日期：2003-09-11 00:00:00

abstract：:In developing ideas of how protein structure modifies haem reactivity, the activity of Class I of the plant peroxidase superfamily (including cytochrome c peroxidase, ascorbate peroxidase and catalase-peroxidases (KatGs)) is an exciting field of research. Despite striking sequence homologies, there are dramatic differ...

journal_title：FEBS letters

authors： Zámocký M,Regelsberger G,Jakopitsch C,Obinger C

更新日期：2001-03-16 00:00:00

abstract：:Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were c...

journal_title：FEBS letters

authors： Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet A

更新日期：1998-11-27 00:00:00

abstract：:A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

journal_title：FEBS letters

authors： Bennett EP,Hassan H,Hollingsworth MA,Clausen H

更新日期：1999-10-29 00:00:00

abstract：:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report ...

journal_title：FEBS letters

authors： Hanada T,Satomi Y,Takao T,Ohsumi Y

更新日期：2009-04-02 00:00:00

abstract：:Coding region and intronic mutations in the gene for microtubule-associated protein tau cause frontotemporal dementia and Parkinsonism linked to chromosome 17 (FTDP-17). Most coding region mutations effect a reduced ability of tau protein to interact with microtubules and lead to the formation of a filamentous patholo...

journal_title：FEBS letters

authors： Smith MJ,Crowther RA,Goedert M

更新日期：2000-11-10 00:00:00

abstract：:Chromatin phospholipidic fraction, as previously demonstrated, shows the same localization as RNA inside the nuclei. DNase and RNase treatment of nuclei removed almost totally the DNA, 63% of RNA and caused a 50% loss of phospholipids. The aim of the present investigation is to study the fraction of RNase undigested n...

journal_title：FEBS letters

authors： Micheli M,Albi E,Leray C,Magni MV

更新日期：1998-07-24 00:00:00

abstract：:The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...

journal_title：FEBS letters

authors： Ito S,Ihara T,Tamura H,Tanaka S,Ikeda T,Kajihara H,Dissanayake C,Abdel-Motaal FF,El-Sayed MA

更新日期：2007-07-10 00:00:00