Human HRD1 protects against ER stress-induced apoptosis through ER-associated degradation.

abstract：:We have attempted to elucidate the mechanism of apoptotic cell death induced by hypoxia (very low oxygen conditions) in neuronal cells. Human neuroblastoma SK-N-MC cells under hypoxic conditions resulted in apoptosis in a time-dependent manner estimated by DNA fragmentation assay and nuclear morphology stained with fl...

journal_title：FEBS letters

authors： Araya R,Uehara T,Nomura Y

更新日期：1998-11-13 00:00:00

abstract：:The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplac...

journal_title：FEBS letters

authors： Kovacevic A,Hammer A,Sundl M,Pfister B,Hrzenjak A,Ray A,Ray BK,Sattler W,Malle E

更新日期：2006-01-09 00:00:00

abstract：:NDRG1 is phosphorylated by SGK1 (but not PKB) in vivo at three residues each contained within three nonapeptide repeats. Here, we demonstrate that this nonapeptide, like the NDRG1 protein, is phosphorylated by SGK1, but not by PKBalpha or RSK1 in vitro. The inability of PKBalpha and RSK1 to phosphorylate the nonapepti...

journal_title：FEBS letters

authors： Murray JT,Cummings LA,Bloomberg GB,Cohen P

更新日期：2005-02-14 00:00:00

abstract：:Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E-box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein ...

journal_title：FEBS letters

authors： Mandolesi G,Gargano S,Pennuto M,Illi B,Molfetta R,Soucek L,Mosca L,Levi A,Jucker R,Nasi S

更新日期：2002-01-02 00:00:00

abstract：:We here cloned a cDNA encoding STAM2, a new member of the STAM family, which contains an SH3 domain and ITAM. STAM2 like STAM1 is associated with Jak2 and Jak3, and involved in the signaling for DNA synthesis and c-myc induction mediated by IL-2 and GM-CSF. Co-expression of the SH3 deletion mutants of STAM1 and STAM2 ...

journal_title：FEBS letters

authors： Endo K,Takeshita T,Kasai H,Sasaki Y,Tanaka N,Asao H,Kikuchi K,Yamada M,Chenb M,O'Shea JJ,Sugamura K

更新日期：2000-07-14 00:00:00

abstract：:In this work, we investigated electron transport around the photosynthetic pigment-protein complex of Photosystem I (PS I) mediated by external high-potential electron carrier 2,3-dichloro-1,4-naphtoquinone (Cl2 NQ) and ascorbate. It has been demonstrated that the oxidized species of Cl2 NQ and ascorbate serve as inte...

journal_title：FEBS letters

authors： Petrova AA,Trubitsin BV,Boskhomdzhieva BK,Semenov AY,Tikhonov AN

更新日期：2018-07-01 00:00:00

abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

journal_title：FEBS letters

authors： Hinsley AP,Stanley NR,Palmer T,Berks BC

更新日期：2001-05-18 00:00:00

abstract：:We previously found a brain-specific glycoprotein in the rat brain. It postnatally increases and is rich in the mature brain. We cloned cDNA of this protein. It is composed of a signal peptide, a V-type immunoglobulin domain, two C1-type immunoglobulin domains, a transmembrane segment and a cytoplasmic region containi...

journal_title：FEBS letters

authors： Sano S,Ohnishi H,Omori A,Hasegawa J,Kubota M

更新日期：1997-07-14 00:00:00

abstract：:The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following eit...

journal_title：FEBS letters

authors： Roberts JM,Kenton P,Johnson PM

更新日期：1990-07-16 00:00:00

abstract：:In tritium-hydrogen exchange experiments, the large GroEL substrate Rubisco was unfolded and exchanged in urea/acid/tritiated water, then diluted into either protic buffer or protic buffer containing GroEL. The respective Rubisco metastable folding intermediate or Rubisco-GroEL binary complex was then separated from r...

journal_title：FEBS letters

authors： Park ES,Fenton WA,Horwich AL

更新日期：2005-02-14 00:00:00

abstract：:The timely and reliable processing of auditory and vestibular information within the inner ear requires highly sophisticated sensory transduction pathways. On a cellular level, these demands are met by hair cells, which respond to sound waves - or alterations in body positioning - by releasing glutamate-filled synapti...

journal_title：FEBS letters

authors： Pangrsic T,Vogl C

更新日期：2018-11-01 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be a site of [Fe-S] cluster biogenesis. Here we report the cloning of lipoic acid synthase (LipA) from Toxoplasma gondii, a well known [Fe-S] protein. It is able to complement a LipA-deficient Escherichia coli strain, clea...

journal_title：FEBS letters

authors： Thomsen-Zieger N,Schachtner J,Seeber F

更新日期：2003-07-17 00:00:00

abstract：:This study was performed to prove our hypothesis that the metabolite(s) of polycyclic aromatic hydrocarbons (PAHs) caused the activation or phosphorylation of p53 via DNA damage to suppress the liver X receptor (LXR)-mediated signal transductions as a probably more direct mechanism. We found that LXR-mediated trans-ac...

journal_title：FEBS letters

authors： Iwano S,Shibahara N,Saito T,Kamataki T

更新日期：2006-02-06 00:00:00

abstract：:A low resolution solution structure of the IIA domain of the Bacillus subtilis phosphoenolpyruvate-sugar phosphotransferase system (PTS) glucose permease has been determined using 945 inter-residue and 724 intra-residue distance constraints derived from three-dimensional 15N and 13C edited NOESY spectra. A total of 15...

journal_title：FEBS letters

authors： Fairbrother WJ,Gippert GP,Reizer J,Saier MH Jr,Wright PE

更新日期：1992-01-20 00:00:00

abstract：:The heterotrophic nitrifier Paracoccus denitrificans expresses a membrane-associated ammonia monooxygenase. The active enzyme has been solubilized in the detergent dodecyl-beta-D-maltoside and purified by standard chromatographic techniques. This is the first purification of an ammonia monooxygenase. The enzyme consis...

journal_title：FEBS letters

authors： Moir JW,Crossman LC,Spiro S,Richardson DJ

更新日期：1996-05-27 00:00:00

abstract：:Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deleti...

journal_title：FEBS letters

authors： Vishnu Varthini L,Selvaraju K,Srinivasan M,Nachiappan V

更新日期：2015-01-02 00:00:00

abstract：:Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...

journal_title：FEBS letters

authors： Loir M,Caraty A,Lanneau M,Menezo Y,Muh JP,Sautiere P

更新日期：1984-04-24 00:00:00

abstract：:RNA silencing as a robust host defense mechanism against plant viruses is generally countered by virus-encoded silencing suppressors. This strategy is now increasingly recognized to be used by animal viruses as well. We present here an overview of the common features shared by some of the better studied plant viral si...

journal_title：FEBS letters

authors： Qu F,Morris TJ

更新日期：2005-10-31 00:00:00

abstract：:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...

journal_title：FEBS letters

authors： Hung F,Luo D,Sauvé DM,Muller MT,Roberge M

更新日期：1996-02-12 00:00:00

abstract：:The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

journal_title：FEBS letters

authors： Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

更新日期：1994-01-17 00:00:00

abstract：:The catalytic cycle of heme peroxidases involves two reactive states, compound I and compound II. Although their reduction potentials at pH 7 are similar, compound I is in general more reactive towards organic substrates than compound II. The different reactivities have until now remained unexplained. In this study, t...

journal_title：FEBS letters

authors： Folkes LK,Candeias LP

更新日期：1997-07-28 00:00:00

abstract：:SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in ...

journal_title：FEBS letters

authors： Law PY,Liu YM,Geng H,Kwan KH,Waye MM,Ho YY

更新日期：2006-06-26 00:00:00

abstract：:The Mahogany/Attractin gene (Atrn) has been proposed as a downstream mediator of Agouti signaling because yellow hair color and obesity in lethal yellow (A(y)) mice are suppressed by the mahogany (Atrn(mg)) mutation. The present study examined the distribution of Atrn mRNA in the brain and spinal cord by in situ hybri...

journal_title：FEBS letters

authors： Lu Xy,Gunn TM,Shieh Kr,Barsh GS,Akil H,Watson SJ

更新日期：1999-11-26 00:00:00

abstract：:The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...

journal_title：FEBS letters

authors： Norris MD,Stewart BW

更新日期：1988-02-15 00:00:00

abstract：:Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separa...

journal_title：FEBS letters

authors： Dobos GJ,Wu XR,Traynor-Kaplan A

更新日期：1993-06-14 00:00:00

abstract：:Dicarbonyl/L-xylulose reductase (DCXR) converts l-xylulose into xylitol, and reduces various α-dicarbonyl compounds, thus performing a dual role in carbohydrate metabolism and detoxification. In this study, we identified DHS-21 as the only DCXR ortholog in Caenorhabditis elegans. The dhs-21 gene is expressed in variou...

journal_title：FEBS letters

authors： Son le T,Ko KM,Cho JH,Singaravelu G,Chatterjee I,Choi TW,Song HO,Yu JR,Park BJ,Lee SK,Ahnn J

更新日期：2011-05-06 00:00:00

abstract：:The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...

journal_title：FEBS letters

authors： Norwood TJ,Boyd J,Campbell ID

更新日期：1989-09-25 00:00:00

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

journal_title：FEBS letters

authors： Birkett CR,Foster KE,Johnson L,Gull K

更新日期：1985-08-05 00:00:00