A naturally occurring bacterial Tat signal peptide lacking one of the 'invariant' arginine residues of the consensus targeting motif.

abstract：:The absorption frequencies of the C = O and C = C (neutral state) and of the C...O (semiquinone state) stretching vibrations of QB have been assigned by FTIR spectroscopy, using native and site-specifically 1-, 2-, 3- and 4-13C-labelled ubiquinone-10 (UQ10) reconstituted at the QB binding site of Rhodobacter sphaeroid...

journal_title：FEBS letters

authors： Brudler R,de Groot HJ,van Liemt WB,Gast P,Hoff AJ,Lugtenburg J,Gerwert K

更新日期：1995-08-14 00:00:00

abstract：:High-molecular-weight [14C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated. [14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (M(r) about 40,000), whereas that o...

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Ikeya H,Higuchi T,Tanaka A,Morikawa A,Saito Y,Takagaki K,Endo M

更新日期：1994-06-20 00:00:00

abstract：:The further biochemical characterization and subcellular localization of cardiotin, a high molecular weight (300 kDa) constituent of cardiac muscle, is described. Immunofluorescence assays revealed a colocalization of cardiotin and the Ca2+ pump SERCA2a in the longitudinal sarcoplasmic reticulum (SR). However, in cont...

journal_title：FEBS letters

authors： Schaart G,Moens L,Endert JM,Ramaekers FC

更新日期：1997-02-17 00:00:00

abstract：:The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

journal_title：FEBS letters

authors： Baldwin GS

更新日期：1995-02-06 00:00:00

abstract：:Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...

journal_title：FEBS letters

authors： Picone D,Temussi PA,Marastoni M,Tomatis R,Motta A

更新日期：1988-04-11 00:00:00

abstract：:The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...

journal_title：FEBS letters

authors： Milnes JT,Dempsey CE,Ridley JM,Crociani O,Arcangeli A,Hancox JC,Witchel HJ

更新日期：2003-07-17 00:00:00

abstract：:The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...

journal_title：FEBS letters

authors： Syroeshkin AV,Vasilyeva EA,Vinogradov AD

更新日期：1995-06-05 00:00:00

abstract：:Integrin avidity is regulated by changes in the conformation of the heterodimer and cluster formation. We measured cell adhesion by integrin alpha2beta1 (CHO-alpha2) to collagen at short contact times (0.5-60s) by single cell force spectroscopy (SCFS). The adhesion increased rapidly with contact time and was further s...

journal_title：FEBS letters

authors： Tulla M,Helenius J,Jokinen J,Taubenberger A,Müller DJ,Heino J

更新日期：2008-10-15 00:00:00

abstract：:Affinity labelling of pancreatic RNase with 4-(N-2-chloroethyl-N-methylamino)benzylamide and (N----P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as sugg...

journal_title：FEBS letters

authors： Knorre DG,Buneva VN,Baram GI,Godovikova TS,Zarytova VF

更新日期：1986-01-01 00:00:00

abstract：:To better understand the outcome of the interaction between TNF-related apoptosis-inducing factor (TRAIL) and tumor cells, we studied TRAIL-resistant melanoma cells resulting from prolonged exposure to TRAIL and found that they had higher proliferative activity than the parental cells both in vitro and in vivo. This w...

journal_title：FEBS letters

authors： Wu JJ,Zhang XD,Gillespie S,Hersey P

更新日期：2005-03-28 00:00:00

abstract：:Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any d...

journal_title：FEBS letters

authors： Obata K,Furuno T,Nakanishi M,Togari A

更新日期：2007-12-22 00:00:00

abstract：:The Rho-guanine nucleotide exchange factors (Rho-GEFs) remodel the actin cytoskeleton via their Rho-GTPase targets and affect numerous physiological processes such as transformation and cell motility. They are therefore attractive targets to design specific inhibitors that may have therapeutic applications. Trio conta...

journal_title：FEBS letters

authors： Schmidt S,Diriong S,Méry J,Fabbrizio E,Debant A

更新日期：2002-07-17 00:00:00

abstract：:Focal adhesion (FA) proteins, kindlin-2 and integrin-linked kinase (ILK), regulate cell adhesion and migration. ILK interacts with and promotes kindlin-2 targeting to FAs. Leu353 and Leu357 in kindlin-2 have been reported to be important for the interaction between kindlin-2 and ILK. However, the binding interface bet...

journal_title：FEBS letters

authors： Guan SY,Chng CP,Ong LT,Tan HF,Alex Law SK,Tan SM

更新日期：2018-01-01 00:00:00

abstract：:Plastidic type I signal peptidase 1 (Plsp1) is necessary for proper chloroplast development although its mode of action is not completely understood. Here, we demonstrate by immunoblotting and electron microscopy immunolocalization that Plsp1 is evenly distributed in the envelope and thylakoids of developing chloropla...

journal_title：FEBS letters

authors： Shipman RL,Inoue K

更新日期：2009-03-04 00:00:00

abstract：:Tryptophan-rich antigens of malarial parasites interact with host molecules and play an important role in parasite survival. Merozoite expressed Plasmodium vivax tryptophan-rich antigen PvTRAg38 binds to human erythrocytes and facilitates parasite growth in a heterlologous Plasmodium falciparum culture system. Recentl...

journal_title：FEBS letters

authors： Alam MS,Rathore S,Tyagi RK,Sharma YD

更新日期：2016-01-01 00:00:00

abstract：:An up to 14-fold increase in total hexokinase activity induced by low-frequency stimulation in rat fast-twitch muscle was followed by a rapid decay in enzyme activity after cessation of stimulation. In vivo labeling revealed that these alterations were related to rapid changes in [35S]methionine incorporation into hex...

journal_title：FEBS letters

authors： Weber FE,Pette D

更新日期：1990-02-26 00:00:00

abstract：:T cells express two different types of voltage-independent Ca(2+)-activated K(+) channels with small (SK) and intermediate (IK) conductance that serve important roles in the activation of T lymphocytes. In contrast to the IK channels from T lymphocytes which are upregulated upon mitogen stimulation, SK channels of Jur...

journal_title：FEBS letters

authors： Jäger H,Adelman JP,Grissmer S

更新日期：2000-03-10 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain FV fragments fused to lipoprotein signal peptide and N-term...

journal_title：FEBS letters

authors： Keinänen K,Laukkanen ML

更新日期：1994-06-06 00:00:00

abstract：:One type of covalent linkages connecting lignin and hemicellulose in plant cell walls is the ester linkage between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols. An enzyme that could hydrolyze such linkages, named glucuronoyl esterase, occurs in the cellulolytic system of the wood-rotting fungus S...

journal_title：FEBS letters

authors： Li XL,Spániková S,de Vries RP,Biely P

更新日期：2007-08-21 00:00:00

abstract：:Chronic indirect stimulation of fast twitch rabbit muscle induced a rapid reduction in parvalbumin concentration. When compared to the unstimulated contralateral muscle, parvalbumins were reduced to 55% following 6 days of stimulation. Prolonged stimulation further reduced parvalbumins so that they were undetectable a...

journal_title：FEBS letters

authors： Klug G,Reichmann H,Pette D

更新日期：1983-02-21 00:00:00

abstract：:The -proteobacterium Wolinella succinogenes grows anaerobically by respiratory nitrite ammonification but not by denitrification. Nevertheless, it is capable of N(2)O reduction to N(2). Recently, the genome sequence of W. succinogenes revealed a nos gene cluster with intriguing features encoding a new type of N(2)O re...

journal_title：FEBS letters

authors： Simon J,Einsle O,Kroneck PM,Zumft WG

更新日期：2004-07-02 00:00:00

abstract：:Changes in troponin C (TnC) conformation upon Ca(2+) binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca(2+)-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-l...

journal_title：FEBS letters

authors： Bukatina AE,Morozov VN,Gusev NB,Sieck GC

更新日期：2002-07-31 00:00:00

abstract：:In presynaptic nerve terminals, synaptic vesicles are recycled locally via an evolutionarily conserved process that ensures maintenance of neurotransmission as well as structural integrity of synapses. Temperature is a key environmental factor that impacts critical steps involved in fusion, endocytosis and transport i...

journal_title：FEBS letters

authors： Chanaday NL,Kavalali ET

更新日期：2018-11-01 00:00:00

abstract：:Cyclic ADP-ribose (cADPR) is a natural metabolite of beta-NAD+ with a potent Ca2+-mobilizing activity in different cell types, including T-lymphocytes. We investigated (i) whether stimulation of T-lymphocytes with different agonists affects the intracellular concentration of cADPR, and (ii) whether the lymphocyte anti...

journal_title：FEBS letters

authors： da Silva CP,Schweitzer K,Heyer P,Malavasi F,Mayr GW,Guse AH

更新日期：1998-11-20 00:00:00

abstract：:The interaction of vinculin with actin filaments was investigated by methods which exclude interference by contaminating proteins which may occur in vinculin preparations. Vinculin which was blotted from SDS-polyacrylamide gels onto nitrocellulose, was stained specifically by fluorescently labeled polymeric actin (100...

journal_title：FEBS letters

authors： Ruhnau K,Wegner A

更新日期：1988-02-08 00:00:00

abstract：:Pyruvate dehydrogenase (E1p) is one of the components of the pyruvate dehydrogenase multienzyme complex (PDHC). Previously, it was shown that the N-terminal domain of E1p is involved in its binding to the core component (E2p) of PDHC. We constructed point mutations in this domain (D17Q, D17R, E20Q, E20R, D24Q and D24R...

journal_title：FEBS letters

authors： Hengeveld AF,de Kok A

更新日期：2002-07-03 00:00:00

abstract：:Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...

journal_title：FEBS letters

authors： Tanfin Z,Goureau O,Milligan G,Harbon S

更新日期：1991-01-14 00:00:00

abstract：:1,3-alpha-D-Glucan synthase of Streptococcus mutans catalyzes both the hydrolysis of sucrose to glucose and fructose, and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan. The enzyme catalyzes only sucrose hydrolysis, however, in the absence of 1,6-alpha-D-glucan as an acceptor. In the presen...

journal_title：FEBS letters

authors： Yamashita Y,Hanada N,Itoh-Andoh M,Takehara T

更新日期：1989-01-30 00:00:00

abstract：:The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase r...

journal_title：FEBS letters

authors： Banas B,Eberle J,Banas B,Schlöndorff D,Luckow B

更新日期：2001-12-07 00:00:00