Apicomplexan parasites contain a single lipoic acid synthase located in the plastid.

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:The capacity of incomplete segments of Escherichia coli lactose permease to form transport-competent complexes in vivo was further tested. Two series of mutant lacY genes were constructed. One encoded N-terminal lactose permease segments of different length. The proteins specified by the other group contained deletion...

journal_title：FEBS letters

authors： Wrubel W,Stochaj U,Ehring R

更新日期：1994-08-08 00:00:00

abstract：:ATM (ataxia telangiectasia mutated), the gene mutated in ataxia telangiectasia, is related to a family of large phosphatidylinositol 3-kinase domain-containing proteins involved in cell cycle control and DNA repair. We found that ATM(-/-) DT40 cells were more susceptible than wild-type cells to apoptosis induced not o...

journal_title：FEBS letters

authors： Takao N,Li Y,Yamamoto K

更新日期：2000-04-21 00:00:00

abstract：:We have recently demonstrated that myocardial adaptation to ischemia triggers a tyrosine kinase regulated signaling pathway leading to the translocation and activation of p38 MAP kinase and MAPKAP kinase 2. Since oxidative stress is developed during ischemic adaptation and since free radicals have recently been shown ...

journal_title：FEBS letters

authors： Maulik N,Sato M,Price BD,Das DK

更新日期：1998-06-16 00:00:00

abstract：:Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

journal_title：FEBS letters

authors： Hübner D,Drakenberg T,Forsén S,Fischer G

更新日期：1991-06-17 00:00:00

abstract：:Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

journal_title：FEBS letters

authors： Groot Kormelink PJ,Luyten WH

更新日期：1997-01-06 00:00:00

abstract：:The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

journal_title：FEBS letters

authors： Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

更新日期：1991-06-24 00:00:00

abstract：:Resonance Raman spectra were observed for compound II of horseradish peroxidase A2, and the Fe(IV) = O stretching Raman line was identified at 775 cm-1. This Raman line shifted to 741 cm-1 upon a change of solvent from H2(16)O to H2(18)O, indicating occurrence of the oxygen exchange between the Fe(IV) = O heme and bul...

journal_title：FEBS letters

authors： Hashimoto S,Nakajima R,Yamazaki I,Tatsuno Y,Kitagawa T

更新日期：1986-11-24 00:00:00

abstract：:Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into ...

journal_title：FEBS letters

authors： Wittelsberger A,Thomas BE,Mierke DF,Rosenblatt M

更新日期：2006-03-20 00:00:00

abstract：:Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...

journal_title：FEBS letters

authors： Blázquez MA,Gamo FJ,Gancedo C

更新日期：1995-12-18 00:00:00

abstract：:The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex...

journal_title：FEBS letters

authors： Guo J,Zhang Z,Bi Y,Yang W,Xu Y,Zhang L

更新日期：2005-07-04 00:00:00

abstract：:We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

journal_title：FEBS letters

authors： Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

更新日期：1999-05-07 00:00:00

abstract：:Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expr...

journal_title：FEBS letters

authors： Kilgour E,Anderson NG

更新日期：1993-08-16 00:00:00

abstract：:A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

journal_title：FEBS letters

authors： Palomäki A,Malminiemi K,Metsä-Ketelä T

更新日期：1997-06-30 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00

abstract：:The plant toxin ricin has proven valuable as a membrane marker in studies of endocytosis as well as studies of different intracellular transport steps. The toxin, which consists of two polypeptide chains, binds by one chain (the B-chain) to both glycolipids and glycoproteins with terminal galactose at the cell surface...

journal_title：FEBS letters

authors： Sandvig K,van Deurs B

更新日期：1999-06-04 00:00:00

abstract：:When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...

journal_title：FEBS letters

authors： van Meer G,Hoetzl S

更新日期：2010-05-03 00:00:00

abstract：:We used a xenograft model to investigate whether the aryl hydrocarbon receptor deletion construct CDelta553 suppresses tumor growth. HeLa cells that were infected with CDelta553 expressing adenovirus (Ad553) formed very small tumors whereas the control adenovirus-infected cells formed large tumors at day 15. CDelta553...

journal_title：FEBS letters

authors： Wang D,Faridi JS,Li Y,Chan WK

更新日期：2009-09-17 00:00:00

abstract：:Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), a novel peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Gly-Leu-Arg-Trp-NH2, and was named Antho-RWamide II. Antho-RWamide II is a neuropeptide. Its st...

journal_title：FEBS letters

authors： Graff D,Grimmelikhuijzen CJ

更新日期：1988-10-24 00:00:00

abstract：:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

journal_title：FEBS letters

authors： Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

更新日期：2012-06-04 00:00:00

abstract：:This paper describes a mechanism of coupling periodate-oxidized nucleosides to proteins. Each of the dialdehyde groups of a periodate-oxidized nucleoside is shown to couple to lysine residues on different protein molecules through Schiff bases, thereby cross-linking different protein molecules, forming a polymer. This...

journal_title：FEBS letters

authors： Senapathy P,Ali MA,Jacob MT

更新日期：1985-10-14 00:00:00

abstract：:We have studied the pharmacological properties of genetically engineered human NK1 tachykinin receptors in which residues at the extracellular surface of the fourth transmembranal domain were substituted with the corresponding amino acids from the NK2 receptor. We show that substitution of G166C:Y167F in the human NK1...

journal_title：FEBS letters

authors： Ciucci A,Palma C,Riitano D,Manzini S,Werge TM

更新日期：1997-10-27 00:00:00

abstract：:Motile flagella are crucial for human fertility and embryonic development. The distal tip of the flagellum is where growth and intra-flagellar transport are coordinated. In most model organisms, but not all, the distal tip includes a 'singlet region', where axonemal doublet microtubules (dMT) terminate and only comple...

journal_title：FEBS letters

authors： Zabeo D,Croft JT,Höög JL

更新日期：2019-05-01 00:00:00

abstract：:In the present study we show the existence of a functional nitric oxide synthase (NOS) in rat liver mitochondria. The enzyme uses L-arginine (L-arg) to produce nitric oxide (NO) and L-citrulline, and is Ca2+-dependent. L-Arg analogues, N(omega)monomethyl-L-arg and N(omega)-nitro-L-arg, inhibit the enzyme, and D-argini...

journal_title：FEBS letters

authors： Ghafourifar P,Richter C

更新日期：1997-12-01 00:00:00

abstract：:miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that s...

journal_title：FEBS letters

authors： Mi W,Shi Q,Chen X,Wu T,Huang H

更新日期：2016-02-01 00:00:00

abstract：:Lipoprotein MtsA is a critical component of MtsABC responsible for iron binding and transport in the Gram-positive bacterium Streptococcus pyogenes. The present collective experimental data establish that Fe(2+) is the primary binding ion for MtsA under optimal physiologically relevant conditions. The binding affiniti...

journal_title：FEBS letters

authors： Sun X,Ge R,Chiu JF,Sun H,He QY

更新日期：2008-04-16 00:00:00

abstract：:Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...

journal_title：FEBS letters

authors： Rouet R,Lowe D,Christ D

更新日期：2014-01-21 00:00:00

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:Rho proteins and phosphatidylinositide 3-kinases (PI 3-kinases) have been widely implicated in regulating cell motility both in cultured cells and in animal models. Monocytes are recruited from the bloodstream in response to inflammatory signals, and migrate across the endothelial barrier into the tissues, where they ...

journal_title：FEBS letters

authors： Ridley AJ

更新日期：2001-06-08 00:00:00

abstract：:The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...

journal_title：FEBS letters

authors： Olsen J,Cowell GM,Kønigshøfer E,Danielsen EM,Møller J,Laustsen L,Hansen OC,Welinder KG,Engberg J,Hunziker W

更新日期：1988-10-10 00:00:00