Abstract:
:In the present study we show the existence of a functional nitric oxide synthase (NOS) in rat liver mitochondria. The enzyme uses L-arginine (L-arg) to produce nitric oxide (NO) and L-citrulline, and is Ca2+-dependent. L-Arg analogues, N(omega)monomethyl-L-arg and N(omega)-nitro-L-arg, inhibit the enzyme, and D-arginine is not a substrate for it. We found mitochondrial NOS (mtNOS) activity associated with the inner mitochondrial membrane but not with the matrix fraction. In intact, succinate-energized mitochondria, the enzyme is constitutively active and exerts substantial control over mitochondrial respiration and membrane potential. The activity is further stimulated when Ca2+ is taken up by mitochondria. We suggest that the existence of mtNOS and its Ca2+ dependence are highly relevant for mitochondrial functioning.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ghafourifar P,Richter Cdoi
10.1016/s0014-5793(97)01397-5subject
Has Abstractpub_date
1997-12-01 00:00:00pages
291-6issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01397-5journal_volume
418pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac-specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro-B BAF ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12189
更新日期:2016-05-01 00:00:00
abstract::Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80374-5
更新日期:1987-08-31 00:00:00
abstract::A cell-free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus. Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double-layered nu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01938-4
更新日期:2000-09-01 00:00:00
abstract::Analysis of 13 high-resolution protein X-ray crystal structures shows that 1204 (24%) of all the 4974 hydrogen bonds are of the bifurcated three-center type with the donor X-H opposing two acceptors A1, A2. They occur systematically in alpha-helices where 90% of the hydrogen bonds are of this type; the major component...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81032-4
更新日期:1991-08-19 00:00:00
abstract::Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01189-3
更新日期:1994-11-28 00:00:00
abstract::The tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02522-x
更新日期:2002-04-10 00:00:00
abstract::Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02283-9
更新日期:2001-03-30 00:00:00
abstract::N-Biotinyl phosphatidylethanolamine spin labelled at the C-14 position of the sn-2 chain has been incorporated at a level of 1 mol% in bilayers of dimyristoyl phosphatidylcholine, and the effects on the chain mobility of binding avidin to the biotin lipid headgroup have been studied by electron spin resonance spectros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81531-4
更新日期:1993-06-07 00:00:00
abstract::Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GD1a and GM3 but also GM2 and GM1, both of wh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80985-1
更新日期:1986-10-06 00:00:00
abstract::We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00491-3
更新日期:1999-05-07 00:00:00
abstract::Spermidine N1-acetyltransferase in an extract from phytohemagglutinin-stimulated bovine lymphocytes was inactivated by preincubation with alkaline phosphatase. Inactivation of the acetylase with the phosphatase was totally inhibited by addition of pyrophosphate. These results suggest that spermidine N1-acetyltransfera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81336-7
更新日期:1982-12-13 00:00:00
abstract::The intestinal epithelium is equipped with sensing receptor mechanisms that interact with luminal microorganisms and nutrients to regulate barrier function and gut immune responses, thereby maintaining intestinal homeostasis. Herein, we clarify the role of the extracellular calcium-sensing receptor (CaSR) using intest...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.05.007
更新日期:2014-11-17 00:00:00
abstract::The dual function of the regulatory beta-subunit of protein kinase CK2 is highlighted by its ability to abolish calmodulin phosphorylation in contrast to its stimulatory effect on the phosphorylation of peptide substrates. Here we show that a synthetic peptide reproducing the C-terminal region of the beta-subunit (bet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00295-k
更新日期:1995-04-17 00:00:00
abstract::LEA (late embryogenesis abundant) proteins are intrinsically disordered proteins that contribute to stress tolerance in plants and invertebrates. Here we show that, when both plant and animal LEA proteins are co-expressed in mammalian cells with self-aggregating polyglutamine (polyQ) proteins, they reduce aggregation ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.020
更新日期:2011-02-18 00:00:00
abstract::Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80723-3
更新日期:1988-04-11 00:00:00
abstract::We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80975-8
更新日期:1984-11-05 00:00:00
abstract::The role of tRNA in translating the genetic code has received considerable attention over the last 50 years, and we now know in great detail how particular amino acids are specifically selected and brought to the ribosome in response to the corresponding mRNA codon. Over the same period, it has also become increasingl...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.013
更新日期:2010-01-21 00:00:00
abstract::Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the...
journal_title:FEBS letters
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.febslet.2013.04.020
更新日期:2013-09-02 00:00:00
abstract::Alternative splicing of the Alzheimer's amyloid beta protein precursor (ABPP) message leads to the production of several variants of this precursor polypeptide. Two of these variants contain a domain that is highly homologous to members of the Kunitz class of protease inhibitors. In order to initiate a study of the ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80926-a
更新日期:1990-07-16 00:00:00
abstract::The transmission of receptor-generated cytoplasmic Ca2+ signals between communicating pancreatic acinar cells has been investigated by comparing patch-clamp recordings of Ca(2+)-dependent Cl- current in internally perfused single cells and small multi-cell clusters. Acetylcholine (50 nM) generates shortlasting repetit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80774-w
更新日期:1991-06-17 00:00:00
abstract::Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S-sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01562-8
更新日期:1999-12-03 00:00:00
abstract::The N-terminal processing of MADH from the bacterium T. versutus and the N-terminal heterogeneity of the isolated alpha subunit of the alpha 2 beta 2 protein complex was demonstrated by a combination of Edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80402-g
更新日期:1993-10-25 00:00:00
abstract::1H-NMR spectroscopy has been applied to identify components in the urine of subjects with a deficiency of the enzyme 3-hydroxy-3-methylglutaryl-CoA lyase. One-dimensional spectra of samples from a pair of non-identical twins with this disorder were very similar and are probably diagnostic. The most intense signals wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81434-x
更新日期:1986-07-14 00:00:00
abstract::The effect on the structure of profilin of phosphatidylinositol 4,5-bisphosphate (PIP2) binding was probed by fluorescence and circular dichroism (CD) spectroscopy. Fluorescence of Trp3 and Trp31 of profilin at 292 nm showed a linear decrease in solution emission at 340 nm as PIP2/profilin was increased from 0 to 80:1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80324-a
更新日期:1992-02-03 00:00:00
abstract::The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.12.033
更新日期:2008-01-23 00:00:00
abstract::Phospholipase C(gamma)2 (PLCgamma2) is a phospholipid-converting enzyme which, upon receptor stimulation, is activated within membrane-bound signalling complexes. In contrast to the highly ubiquitous PLCgamma1, PLCgamma2 is expressed predominantly in B-lymphocytes. Associated with antigen-coupling receptors it is acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01276-8
更新日期:1996-12-09 00:00:00
abstract::The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02677-7
更新日期:2002-05-22 00:00:00
abstract::Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80398-e
更新日期:1993-10-25 00:00:00
abstract::Redox reactions of tyrosine play key roles in many biological processes, including water oxidation and DNA synthesis. We first review the redox properties of tyrosine (and other phenols) in small molecules and related polypeptides, then report work on (H20)/(Y48)-modified Pseudomonas aeruginosa azurin. The crystal str...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.12.014
更新日期:2012-03-09 00:00:00
abstract::The present one-drug-one-target paradigm in drug discovery has been considered partially responsible for the more-funding-less-drug predicament in modern pharmaceutical industry. To hit the multiple targets implicated in complex diseases, two strategies, based on multicomponent or single-ingredient, are conceivable. A...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.09.006
更新日期:2005-10-10 00:00:00