A modified nicotinic acetylcholine receptor lacking the 'ion channel amphipathic helices'.

abstract：:The temperature dependence of the rate constants of the univalent redox steps YzoxSi----YzSi + 1 (i = 0,1,2) and YzoxS3----(YzS4)----YzSo + O2 in the water oxidase was investigated by measuring time resolved absorption changes at 355 nm induced by a laser flash train in dark adapted PS II membrane fragments from spina...

journal_title：FEBS letters

authors： Renger G,Hanssum B

更新日期：1992-03-24 00:00:00

abstract：:Zinc cations at concentrations of 0.2 mM and greater catalyzed specific phosphorylation, by ATP, of two membrane-associated proteins from rat hippocampus. These proteins, corresponding to molecular weights of 60 and 49 kDa, were phosphorylated primarily at tyrosine residues. The 60-kDa protein was identified as p60c-s...

journal_title：FEBS letters

authors： Vener AV,Loeb J

更新日期：1992-06-01 00:00:00

abstract：:An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-a...

journal_title：FEBS letters

authors： Hokke CH,Kamerling JP,van Dedem GW,Vliegenthart JF

更新日期：1991-07-29 00:00:00

abstract：:Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The...

journal_title：FEBS letters

authors： Guinec N,Dalet-Fumeron V,Pagano M

更新日期：1992-08-24 00:00:00

abstract：:U46619 is a potent platelet agonist, its binding to the thromboxane A2 receptor resulting in Gq-binding protein-mediated responses; nevertheless, it is unable to cause platelet aggregation, unless released ADP is present. In this study we demonstrate that Gi activation is the step U46619 lacks to cause platelet aggreg...

journal_title：FEBS letters

authors： Pulcinelli FM,Pesciotti M,Pignatelli P,Riondino S,Gazzaniga PP

更新日期：1998-09-11 00:00:00

abstract：:A protein factor which inhibited adenylate cyclase was purified to apparent homogeneity from rat brain and identified as the beta gamma-subunits of the GTP-binding regulatory proteins of adenylate cyclase. (i) The beta gamma-subunits (protein factor) inhibited the partially purified catalytic unit of adenylate cyclase...

journal_title：FEBS letters

authors： Enomoto K,Asakawa T

更新日期：1986-06-23 00:00:00

abstract：:The formation of fibrils by amyloid beta-protein (Abeta) is considered as a key step in the pathology of Alzheimer's disease (AD). Inhibiting the aggregation of Abeta is a promising approach for AD therapy. In this study, we used biocompatible nanogels composed of a polysaccharide pullulan backbone with hydrophobic ch...

journal_title：FEBS letters

authors： Ikeda K,Okada T,Sawada S,Akiyoshi K,Matsuzaki K

更新日期：2006-12-11 00:00:00

abstract：:The aim of this work was to provide an overview of the genetics of human ageing to gain novel insights about the mechanisms involved. By incorporating findings from model organisms to humans, such as mutations that either delay or accelerate ageing in mice, we constructed the gene networks previously related to ageing...

journal_title：FEBS letters

authors： de Magalhães JP,Toussaint O

更新日期：2004-07-30 00:00:00

abstract：:EcL-DER, the aspartate/glutamate racemase from the pathogen Escherichia coli O157, exhibits racemase activity for l-aspartate and l-glutamate. This study reports the crystal structures of apo-EcL-DER, the EcL-DER-l-aspartate and the EcL-DER-d-aspartate complexes. The EcL-DER structure contains two domains, forming pse...

journal_title：FEBS letters

authors： Liu X,Gao F,Ma Y,Liu S,Cui Y,Yuan Z,Kang X

更新日期：2016-04-01 00:00:00

abstract：:Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing...

journal_title：FEBS letters

authors： Sippel M,Rajala R,Korhonen L,Bornhauser B,Sokka AL,Naito M,Lindholm D

更新日期：2009-07-07 00:00:00

abstract：:The non-ionic detergent Brij 58P is recommended as a stabilizing agent for protein storage; for example, the aggregation-prone chaperone DnaJ can be maintained in solution by low concentrations of Brij 58P. During protein folding studies with alpha-glucosidase, rhodanese and citrate synthase as model proteins, we disc...

journal_title：FEBS letters

authors： Krause M,Rudolph R,Schwarz E

更新日期：2002-12-04 00:00:00

abstract：:Atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP)-activated guanylate cyclases are single-chain transmembrane-spanning proteins, containing both ligand binding and catalytic activities. In both proteins, ligand binding to the extracellular receptor domain activates the cytosolic catalytic domain, ge...

journal_title：FEBS letters

authors： Duda T,Goraczniak RM,Sharma RK

更新日期：1993-12-13 00:00:00

abstract：:We have identified three overlapping 5'-truncated mouse opsin cDNA clones by immunologically screening a lambda gt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was int...

journal_title：FEBS letters

authors： Baehr W,Falk JD,Bugra K,Triantafyllos JT,McGinnis JF

更新日期：1988-10-10 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:A novel calcium-dependent serine proteinase (CASP) secreted from malignant hamster embryo fibroblast Ni 12C2 degrades extracellular matrix proteins. A complementary DNA encoding CASP has been isolated with the use of oligonucleotide probes synthesized based on partial amino acid sequences of CASP. The complete amino a...

journal_title：FEBS letters

authors： Kinoshita H,Sakiyama H,Tokunaga K,Imajoh-Ohmi S,Hamada Y,Isono K,Sakiyama S

更新日期：1989-07-03 00:00:00

abstract：:We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released wi...

journal_title：FEBS letters

authors： Babinski K,Féthière J,Roy M,De Léan A,Ong H

更新日期：1992-11-30 00:00:00

abstract：:We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...

journal_title：FEBS letters

authors： Yano Y,Matsuzaki K

更新日期：2011-07-21 00:00:00

abstract：:Nanoscale protein supported phospholipid bilayer discs, or Nanodiscs, were produced for the purpose of studying the phase transition behavior of the incorporated lipids. Nanodiscs and vesicles were prepared with two phospholipids, dipalmitoyl phosphatidylcholine and dimyristoyl phosphatidylcholine, and the phase trans...

journal_title：FEBS letters

authors： Shaw AW,McLean MA,Sligar SG

更新日期：2004-01-02 00:00:00

abstract：:Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...

journal_title：FEBS letters

authors： Zelenin AV,Kolesnikov VA,Tarasenko OA,Shafei RA,Zelenina IA,Mikhailov VV,Semenova ML,Kovalenko DV,Artemyeva OV,Ivaschenko TE,Evgrafov OV,Dickson G,Baranovand VS

更新日期：1997-09-08 00:00:00

abstract：:In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...

journal_title：FEBS letters

authors： Fox MT,Harriott P,Walker B,Stone SR

更新日期：1997-11-17 00:00:00

abstract：:A structural tree for proteins and domains containing S-like beta-sheets has been constructed. An S-like beta-sheet is taken as a starting structure in modelling or as a root structure of the tree. Larger structures are obtained by a stepwise addition of beta-strands and/or alpha-helices to the root S-like beta-sheet ...

journal_title：FEBS letters

authors： Efimov AV

更新日期：1998-10-23 00:00:00

abstract：:N-Biotinyl phosphatidylethanolamine spin labelled at the C-14 position of the sn-2 chain has been incorporated at a level of 1 mol% in bilayers of dimyristoyl phosphatidylcholine, and the effects on the chain mobility of binding avidin to the biotin lipid headgroup have been studied by electron spin resonance spectros...

journal_title：FEBS letters

authors： Swamy MJ,Marsh D

更新日期：1993-06-07 00:00:00

abstract：:ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...

journal_title：FEBS letters

authors： Li Z,Liu D

更新日期：2012-05-07 00:00:00

abstract：:Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...

journal_title：FEBS letters

authors： Segond N,Jullienne A,Tahri EH,Garel JM

更新日期：1984-08-20 00:00:00

abstract：:Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription fac...

journal_title：FEBS letters

authors： Cao X,Liu D,Yan X,Zhang Y,Yuan L,Zhang T,Fu M,Zhou Y,Wang J

更新日期：2013-03-18 00:00:00

abstract：:The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...

journal_title：FEBS letters

authors： Schofield JG

更新日期：1983-08-08 00:00:00

abstract：:The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...

journal_title：FEBS letters

authors： Kirschke H,Wikstrom P,Shaw E

更新日期：1988-02-08 00:00:00

abstract：:Prepro-vasoactive intestinal peptide (prepro VIP) was expressed in NIH 3T3 cells, and the prepro VIP-derived peptides produced by the cells were analyzed by chromatography combined with sequence-specific radio-immunoanalysis. In accordance with what has previously been reported on processing in non-endocrine cell line...

journal_title：FEBS letters

authors： Wulff BS,Georg B,Fahrenkrug J

更新日期：1994-03-14 00:00:00

abstract：:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

journal_title：FEBS letters

authors： Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

更新日期：1999-03-19 00:00:00

abstract：:10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

journal_title：FEBS letters

authors： Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

更新日期：1996-12-16 00:00:00