Isolation and analysis of the mouse opsin gene.


:We have identified three overlapping 5'-truncated mouse opsin cDNA clones by immunologically screening a lambda gt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was interrupted by four introns positioned precisely as those previously described for other mammalian opsins. In contrast to the single major opsin mRNA in the bovine and human retina, Northern analysis of mouse retina RNA demonstrated the presence of at least five distinct species of polyadenylated opsin mRNAs. Their sizes ranged from 1.7 kb to 5.1 kb.




FEBS letters


Baehr W,Falk JD,Bugra K,Triantafyllos JT,McGinnis JF




Has Abstract


1988-10-10 00:00:00














  • Recognition of an antiparallel beta-sheet structure of human epidermal growth factor by its receptor. Site-directed mutagenesis studies of Ala-30 and Asn-32.

    abstract::The Ala-30 and Asn-32 residues involved in the major antiparallel beta-sheet structure of human epidermal growth factor (hEGF) were substituted with various amino acid residues, and the receptor-binding affinities of the nine variant hEGFs were determined by the use of human KB cells. The Ala-30----Arg, Ala-30----His ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koide H,Muto Y,Kasai H,Hoshi K,Takusari H,Kohri K,Takahashi S,Sasaki T,Tsukumo K,Miyake T

    更新日期:1992-05-04 00:00:00

  • Evidence for a monooxygenase-catalyzed primary process in the catabolism of chlorophyll.

    abstract::Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Curty C,Engel N,Gossauer A

    更新日期:1995-05-01 00:00:00

  • Regulation of lipid synthesis in oil crops.

    abstract::Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Harwood JL,Guschina IA

    更新日期:2013-06-27 00:00:00

  • Methionine acts as a "magnet" in photoaffinity crosslinking experiments.

    abstract::Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wittelsberger A,Thomas BE,Mierke DF,Rosenblatt M

    更新日期:2006-03-20 00:00:00

  • On the role of N-7-mercaptoheptanoyl-O-phospho-L-threonine (component B) in the enzymatic reduction of methyl-coenzyme M to methane.

    abstract::The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ellermann J,Kobelt A,Pfaltz A,Thauer RK

    更新日期:1987-08-17 00:00:00

  • Characterization of yeast fructose-2,6-bisphosphate 6-phosphatase.

    abstract::To obtain information on the biological significance of yeast fructose-2,6-bisphosphate 6-phosphatase, kinetic data of the purified enzyme [(1987) Eur. J. Biochem. 164, 27-30] have been measured. Maximal activity was found between pH 6 and 7, the apparent Michaelis constant with fructose 2,6-bisphosphate was 7.2 micro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Plankert U,Purwin C,Holzer H

    更新日期:1988-10-24 00:00:00

  • Expression cloning of a sheep adreno-ferredoxin using the polymerase chain reaction.

    abstract::In addition to the selective amplification of cDNA from total RNA by the PCR method, the distinctive properties of ferredoxin-expressing colonies can be used for cloning a ferredoxin cDNA. This strategy for cloning and expressing cDNA in E. coli was applied to a sheep adreno-ferredoxin. The expressed sheep ferredoxin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsuo Y,Tsujita M,Mizoguchi K,Ichikawa Y

    更新日期:1992-04-20 00:00:00

  • Transforming growth factor alpha protects against Fas-mediated liver apoptosis in mice.

    abstract::The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kanda D,Takagi H,Toyoda M,Horiguchi N,Nakajima H,Otsuka T,Mori M

    更新日期:2002-05-22 00:00:00

  • The effect of the natural protein inhibitor on H+-ATPase hepatoma 22a mitochondria.

    abstract::The uncoupler-induced inactivation of H+-ATPase in hepatoma 22a and mouse liver mitochondria has been studied. The dependence of this process on delta microH, and pH and ATP was established. The inactivated ATPase could be reactivated at alkaline pH values in the absence of ATP. These data indicate that the inactivati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chernyak BV,Dukhovich VF,Khodjaev EYu

    更新日期:1987-05-11 00:00:00

  • Insulin potentiates the transactivation potency of the glucocorticoid receptor.

    abstract::A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid R...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Georgakopoulos A,Tsawdaroglou N

    更新日期:1996-03-04 00:00:00

  • In situ observation of streptavidin-biotin binding on an immunoassay well surface using an atomic force microscope.

    abstract::Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions. We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Allen S,Davies J,Dawkes AC,Davies MC,Edwards JC,Parker MC,Roberts CJ,Sefton J,Tendler SJ,Williams PM

    更新日期:1996-07-22 00:00:00

  • Sphingosine-1-phosphate: signaling inside and out.

    abstract::Ample evidence indicates that sphingosine-1-phosphate (SPP) can serve as an intracellular second messenger regulating calcium mobilization, and cell growth and survival. Moreover, the dynamic balance between levels of the sphingolipid metabolites, ceramide and SPP, and consequent regulation of opposing signaling pathw...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Spiegel S,Milstien S

    更新日期:2000-06-30 00:00:00

  • Apolipoprotein oxidation in the absence of lipid peroxidation enhances LDL uptake by macrophages.

    abstract::A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

    更新日期:1994-08-08 00:00:00

  • Peroxisomal-microsomal communication in unsaturated fatty acid metabolism.

    abstract::The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baykousheva SP,Luthria DL,Sprecher H

    更新日期:1995-06-26 00:00:00

  • Prokaryotic origins for the mitochondrial alternative oxidase and plastid terminal oxidase nuclear genes.

    abstract::The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Finnegan PM,Umbach AL,Wilce JA

    更新日期:2003-12-18 00:00:00

  • Recognition of transition metal ions by peptides. Identification of specific metal-binding peptides in proteolytic digest maps by UV laser desorption time-of-flight mass spectrometry.

    abstract::Metal-binding peptides in proteolytic digest maps have been identified by matrix-assisted UV laser desorption time-of-flight mass spectrometry (LDTOF-MS). The plasma and milk metal transport protein chosen to demonstrate this process, histidine-rich glycoprotein (HRG), was purified and then digested with trypsin; the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hutchens TW,Nelson RW,Yip TT

    更新日期:1992-01-13 00:00:00

  • Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA.

    abstract::The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (Mr 40456). The amino acid sequen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Didsbury JR,Ho YS,Snyderman R

    更新日期:1987-01-26 00:00:00

  • Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells.

    abstract::The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yogo K,Ogawa T,Akiyama M,Ishida N,Takeya T

    更新日期:2002-11-06 00:00:00

  • Phenylalanine ammonia-lyase immobilized in semipermeable microcapsules for enzyme replacement in phenylketonuria.

    abstract::Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bourget L,Chang TM

    更新日期:1985-01-21 00:00:00

  • Regulation of the type II oncostatin M receptor expression in lung-derived epithelial cells.

    abstract::Oncostatin M (OSM) is a potent modulator of human lung-derived epithelial cell function. This cytokine binds two distinct receptor complexes: type I OSM receptor which is also a functional receptor for leukemia inhibitory factor (LIF), and type II OSM-specific receptor. The role of these two distinct receptors in medi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cichy J,Rose-John S,Puré E

    更新日期:1998-06-16 00:00:00

  • In vitro studies on origin and site of action of enzyme activity responsible for conversion of human proapoprotein A-I into apoprotein A-I.

    abstract::Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

    更新日期:1984-05-07 00:00:00

  • The farnesoid X receptor induces very low density lipoprotein receptor gene expression.

    abstract::The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sirvent A,Claudel T,Martin G,Brozek J,Kosykh V,Darteil R,Hum DW,Fruchart JC,Staels B

    更新日期:2004-05-21 00:00:00

  • Gln(84) of moloney murine leukemia virus reverse transcriptase regulates the incorporation rates of ribonucleotides and deoxyribonucleotides.

    abstract::Moloney murine leukemia virus reverse transcriptase (RT) selectively uses deoxyribonucleotides over ribonucleotides (rNTPs) as substrates. Substitution of F155 with valine (F155V) was previously found to increase the enzyme's affinity for rNTPs, though without affecting the V(max) for catalysis, and thereby conferred ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Liu S,Goff SP,Gao G

    更新日期:2006-02-20 00:00:00

  • Mechanically induced electrical responses in murine mammary epithelial cells in primary culture.

    abstract::In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 mV with a duration of 1-8 s and a subsequent, prominent hyperpolarization of 6 mV lasting 10-40 s. Epidermal growth factor induces a spontaneous hy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Enomoto K,Furuya K,Maeno T,Edwards C,Oka T

    更新日期:1987-10-19 00:00:00

  • The functional role of the beta-subunit in the maturation and intracellular transport of Na,K-ATPase.

    abstract::The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Geering K

    更新日期:1991-07-22 00:00:00

  • Two antifungal thaumatin-like proteins from barley grain.

    abstract::Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hejgaard J,Jacobsen S,Svendsen I

    更新日期:1991-10-07 00:00:00

  • Dynamic structure of pharaonis phoborhodopsin (sensory rhodopsin II) and complex with a cognate truncated transducer as revealed by site-directed 13C solid-state NMR.

    abstract::We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Arakawa T,Shimono K,Yamaguchi S,Tuzi S,Sudo Y,Kamo N,Saitô H

    更新日期:2003-02-11 00:00:00

  • Catechin derivatives: specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels.

    abstract::Tea-catechin derivatives are shown to inhibit activities of caspases-3, 2 and 7 in vitro, and prevented experimental apoptosis at the cell and animal levels. Epigallo-catechin-gallate showed the strongest inhibition at 1 x 10(-7)M to these caspases, but cysteine cathepsins and caspase-8 were not inhibited. Caspase-3 i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katunuma N,Ohashi A,Sano E,Ishimaru N,Hayashi Y,Murata E

    更新日期:2006-02-06 00:00:00

  • Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.

    abstract::In vitro cultured blood cells for transfusion purposes provide a safe alternative to donor blood, particularly for patients who require recurrent transfusions, and can be used as carriers of therapeutic molecules. In vitro derivation of hematopoietic cell types from human-induced pluripotent stem cells (iPSCs) allows ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Hansen M,von Lindern M,van den Akker E,Varga E

    更新日期:2019-12-01 00:00:00

  • A chloroplastic RNA-binding protein is a new member of the PPR family.

    abstract::P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lahmy S,Barnèche F,Derancourt J,Filipowicz W,Delseny M,Echeverria M

    更新日期:2000-09-01 00:00:00