Abstract:
:An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by 1H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hokke CH,Kamerling JP,van Dedem GW,Vliegenthart JFdoi
10.1016/0014-5793(91)80931-rsubject
Has Abstractpub_date
1991-07-29 00:00:00pages
18-24issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80931-Rjournal_volume
286pub_type
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