Abstract:
:Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and planar crystals appeared. The crystals of native P-450scc were also obtained by the same procedure. We identified the crystals as the P-450scc-PLP complex or native P-450scc by absorption spectroscopy and SDS-polyacrylamide gel electrophoresis, and characterized them under a polarization microscope.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Iwamoto Y,Tsubaki M,Hiwatashi A,Ichikawa Ydoi
10.1016/0014-5793(88)81350-4subject
Has Abstractpub_date
1988-06-06 00:00:00pages
31-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(88)81350-4journal_volume
233pub_type
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