Abstract:
:To detect and characterize ion channel activity in the nuclear envelope of a higher plant cell, we performed patch clamp experiments on nuclei isolated from coconut endosperm cells and on giant liposomes containing nuclear envelope fragments prepared from the same cells. An ion channel exhibiting a number of conductance substates, with a maximum of ca. 1,000 pS, was observed. Above an applied potential of +/- 100 mV, the behavior of the channel was similar in isolated nuclei and liposomes, indicating that both patch clamp modes were detecting the same channel. That such a channel has now been identified in members of both the animal and plant kingdoms reinforces the notion that the nuclear pores are not always open to ions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Matzke AJ,Behensky C,Weiger T,Matzke MAdoi
10.1016/0014-5793(92)80290-wkeywords:
subject
Has Abstractpub_date
1992-05-04 00:00:00pages
81-5issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(92)80290-Wjournal_volume
302pub_type
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