The C-terminal domain of yeast Ero1p mediates membrane localization and is essential for function.

abstract：:Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) plays an important role in arachidonate pathway. To investigate the contribution of cPLA(2)alpha to autoimmune diabetes, we established non-obese diabetic (NOD) mouse, an excellent model for human type 1 diabetes, deficient in cPLA(2)alpha. These mice showed severe insu...

journal_title：FEBS letters

authors： Oikawa Y,Yamato E,Tashiro F,Yamamoto M,Uozumi N,Shimada A,Shimizu T,Miyazaki J

更新日期：2005-07-18 00:00:00

abstract：:This study reports for the first time (a) the longitudinal profile of the transmembrane potential (mDeltapsi) of single mitochondria using a Nernstian fluorescent probe and (b) the distribution of mDeltapsi fluctuations of mitochondria undergoing permanent depolarization. Our findings show that (1) mitochondria in dif...

journal_title：FEBS letters

authors： Diaz G,Falchi AM,Gremo F,Isola R,Diana A

更新日期：2000-06-23 00:00:00

abstract：:Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluoresc...

journal_title：FEBS letters

authors： Rephaeli A,Aviram A,Englender T,Shaklai M

更新日期：1988-08-29 00:00:00

abstract：:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

journal_title：FEBS letters

authors： Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

更新日期：2006-03-06 00:00:00

abstract：:We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...

journal_title：FEBS letters

authors： Tabuchi Y,Takasaki I,Doi T,Ishii Y,Sakai H,Kondo T

更新日期：2006-05-29 00:00:00

abstract：:Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...

journal_title：FEBS letters

authors： Tilly BC,Gaestel M,Engel K,Edixhoven MJ,de Jonge HR

更新日期：1996-10-21 00:00:00

abstract：:A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) fro...

journal_title：FEBS letters

authors： Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE

更新日期：1990-10-15 00:00:00

abstract：:In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...

journal_title：FEBS letters

authors： Ordiz I,Herrero P,Rodicio R,Moreno F

更新日期：1995-07-03 00:00:00

abstract：:The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/...

journal_title：FEBS letters

authors： Sinnarajah S,Ezeh PI,Pathirana S,Moss AG,Morrison EE,Vodyanoy V

更新日期：1998-04-24 00:00:00

abstract：:Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...

journal_title：FEBS letters

authors： Wang Y,Han R,Zhang W,Yuan Y,Zhang X,Long Y,Mi H

更新日期：2008-03-05 00:00:00

abstract：:Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...

journal_title：FEBS letters

authors： Darrouzet E,Issartel JP,Lunardi J,Dupuis A

更新日期：1998-07-10 00:00:00

abstract：:Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH(4)(+) ...

journal_title：FEBS letters

authors： Neuhäuser B,Dynowski M,Ludewig U

更新日期：2009-09-03 00:00:00

abstract：:Ileal bile acid-binding protein (I-BABP) is a 14 kDa cytosolic protein which binds bile acids with a high affinity. It is thought to be implicated in the enterohepatic circulation of bile acids and, hence, in cholesterol homeostasis. Using a combination of in vivo and in vitro experiments, we have recently shown that ...

journal_title：FEBS letters

authors： Landrier JF,Grober J,Demydchuk J,Besnard P

更新日期：2003-10-23 00:00:00

abstract：:We have raised monoclonal antibodies (Mab) to the Mr 55,000 desmin polypeptide, electrophoretically purified from cytoskeletal preparations of isolated bovine heart Purkinje fibers. One of the Mabs, 39AB6, revealed desmin only in cow Purkinje fibers and did not react with desmins from other muscle cells, including ven...

journal_title：FEBS letters

authors： Virtanen I,Närvänen O,Thornell LE

更新日期：1990-07-02 00:00:00

abstract：:Ca(2+)-dependent protein kinases (CDPKs) play an essential role in plant Ca(2+)-mediated signal transduction. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. Eight of them were reported previously to be inducible by different stress sti...

journal_title：FEBS letters

authors： Wan B,Lin Y,Mou T

更新日期：2007-03-20 00:00:00

abstract：:Studies of the inhibition of elastases at a molecular level have resulted in the identification of protected dipeptides which are reversible and highly specific inhibitors of human leucocyte elastase (HLE). These have been further developed by increasing their hydrophilicity and potency to give a new family of elastas...

journal_title：FEBS letters

authors： Hassall CH,Johnson WH,Kennedy AJ,Roberts NA

更新日期：1985-04-22 00:00:00

abstract：:FabH (beta-ketoacyl-acyl carrier protein synthase III) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. We report the crystal st...

journal_title：FEBS letters

authors： Gajiwala KS,Margosiak S,Lu J,Cortez J,Su Y,Nie Z,Appelt K

更新日期：2009-09-03 00:00:00

abstract：:The isolation of a novel biologically active peptide, designated galanin, is described. The peptide was discovered by the detection of its C-terminal amide structure in porcine intestinal extract using a chemical method. It was found that galanin consists of 29 amino acids and the complete amino acid sequence is: Gly-...

journal_title：FEBS letters

authors： Tatemoto K,Rökaeus A,Jörnvall H,McDonald TJ,Mutt V

更新日期：1983-11-28 00:00:00

abstract：:Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...

journal_title：FEBS letters

authors： Sugawara K,Fujikawa M,Yoshida M

更新日期：2013-11-29 00:00:00

abstract：:In the present study we assessed the contribution of acidic sphingomyelinase (ASMase), a ceramide generating enzyme, in tumor necrosis factor (TNF)-mediated apoptosis in human colon HT-29 cells. TNF induced apoptosis in HT-29 cells in a time- and dose-dependent fashion. Downregulation of the active endogenous ASMase f...

journal_title：FEBS letters

authors： Colell A,Morales A,Fernández-Checa JC,García-Ruiz C

更新日期：2002-08-28 00:00:00

abstract：:E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...

journal_title：FEBS letters

authors： Lissin NM,Hemmingsen SM

更新日期：1993-06-07 00:00:00

abstract：:A major difference between porcine and bovine pancreatic phospholipase A2 (PA2) is the relatively low affinity of the bovine enzyme for lipid-water interfaces. We have investigated the binding of porcine, bovine, and equine PA2 to n-hexadecylphosphocholine (C16-PC) micelles using optically detected magnetic resonance ...

journal_title：FEBS letters

authors： Mao SY,Maki AH,de Haas GH

更新日期：1987-01-19 00:00:00

abstract：:The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...

journal_title：FEBS letters

authors： Gibson LC,Hunter CN

更新日期：1994-09-26 00:00:00

abstract：:In higher eukaryotes, nine aminoacyl-tRNA synthetases form a multienzyme complex also comprising the three auxiliary proteins p18, p38 and p43, of apparent molecular masses of 18, 38 and 43 kDa. The function of these proteins, invariably found associated to the synthetase components of the complex, is unknown. In orde...

journal_title：FEBS letters

authors： Quevillon S,Mirande M

更新日期：1996-10-14 00:00:00

abstract：:Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surfac...

journal_title：FEBS letters

authors： Jeffrey IW,Flint DJ,Vernon RG,Mayer RJ

更新日期：1985-03-25 00:00:00

abstract：:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

journal_title：FEBS letters

authors： Shannon MF,Wigley PL,Wells JR

更新日期：1985-07-08 00:00:00

abstract：:Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor speci...

journal_title：FEBS letters

authors： Jans DA,Pavo I,Fahrenholz F

更新日期：1993-01-04 00:00:00

abstract：:Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...

journal_title：FEBS letters

authors： Kryukov VM,Ksenzenko VN,Kaliman AV,Bayev AA

更新日期：1983-07-11 00:00:00

abstract：:The conformation change of Thermus thermophilus tRNA(1Ile) upon complex formation with T. thermophilus elongation factor Tu (EF-Tu) was studied by analysis of the circular dichroism (CD) bands at 315 nm (due to the 2-thioribothymidine residue in the T-loop) and at 295 nm (due to the core structure of tRNA). Formation ...

journal_title：FEBS letters

authors： Haruki M,Matsumoto R,Hara-Yokoyama M,Miyazawa T,Yokoyama S

更新日期：1990-04-24 00:00:00

abstract：:'Peripheral' binding sites for benzodiazepines are under neural or homonal control in the pineal gland, olfactory bulb, and kidney. These observations prompted a search for an endogenous substance which could modulate these sites under physiological conditions. Acidified methanol extracts from several tissues (e.g. st...

journal_title：FEBS letters

authors： Mantione CR,Weissman BA,Goldman ME,Paul SM,Skolnick P

更新日期：1984-10-15 00:00:00