Protective role for cytosolic phospholipase A2alpha in autoimmune diabetes of mice.

abstract：:The molecular organisation of the Prochlorococcus marinus rnpB gene and the catalytic activity of the encoded RNA were characterised. Kinetic parameters for several pre-tRNA substrates were comparable to those from other eubacterial RNase P RNAs, although unusually high cation concentrations were required. The CCA-end...

journal_title：FEBS letters

authors： Hess WR,Fingerhut C,Schön A

更新日期：1998-07-17 00:00:00

abstract：:The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...

journal_title：FEBS letters

authors： Giles NM,Gutowski NJ,Giles GI,Jacob C

更新日期：2003-01-30 00:00:00

abstract：:In temperature-induced Triton X-114 phase separation experiments the beta-glucan elicitor-binding site from soybean (Glycine max L.) root membranes was identified as (a) hydrophobic membrane protein(s). The Zwittergent 3-12-solubilized beta-glucan-binding proteins were incorporated into lipid vesicles by the detergent...

journal_title：FEBS letters

authors： Mithöfer A,Ebel J

更新日期：1999-09-17 00:00:00

abstract：:In C4 plants the activity of phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is regulated by phosphorylation/dephosphorylation which is mediated by light/dark signals. The study using protein kinase inhibitors showed that the inhibition pattern of maize PEPC-protein kinase (PEPC-PK) is similar to that of myosin li...

journal_title：FEBS letters

authors： Ogawa N,Okumura S,Izui K

更新日期：1992-05-04 00:00:00

abstract：:Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...

journal_title：FEBS letters

authors： Gether U,Marray T,Schwartz TW,Johansen TE

更新日期：1992-01-27 00:00:00

abstract：:Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi...

journal_title：FEBS letters

authors： Aggarwal BB,Pocsik E,Totpal K

更新日期：1995-09-18 00:00:00

abstract：:The mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes syndrome (MELAS) is a rare congenital disorder of mitochondrial DNA. Five single nucleotide substitutions within the human mitochondrial tRNALeu(UUR) gene have been reported to be associated with MELAS. Here, we provide in vitro evide...

journal_title：FEBS letters

authors： Hao R,Yao YN,Zheng YG,Xu MG,Wang ED

更新日期：2004-12-03 00:00:00

abstract：:The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains thre...

journal_title：FEBS letters

authors： Gaston K,Fried M

更新日期：1994-06-27 00:00:00

abstract：:Epidermal growth factor (EGF) induces transformed phenotypes in EGF receptor-overexpressing NIH3T3 (ER12) cells. Tyrosine kinase inhibitors such as erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate inhibited the EGF-induced phenotypic changes in these cells; while 5'-O-methylerbstatin, an inactive analog...

journal_title：FEBS letters

authors： Umezawa K,Sugata D,Yamashita K,Johtoh N,Shibuya M

更新日期：1992-12-21 00:00:00

abstract：:The effects of the quaternary agent meproadifen on ACh-activated channel currents were studied on myoballs cultured from hind limb muscles of neonatal rats. Meproadifen (0.02-0.1 microM) combined with ACh (0.1-0.3 microM) in the patch pipette caused an increase, followed by a decrease, in the frequency of channel open...

journal_title：FEBS letters

authors： Aracava Y,Albuquerque EX

更新日期：1984-09-03 00:00:00

abstract：:Aging is accompanied by troubles resulting from changes in hormonal and nutritional status. Therefore, the abundance of mRNA coding for triiodothyronine (TR) and retinoic acid (RA) nuclear receptors was studied in the brain of young, adult and aged (2.5, 6 and 24 months, respectively) rats. In the brain of aged rats, ...

journal_title：FEBS letters

authors： Enderlin V,Alfos S,Pallet V,Garcin H,Azaïs-Braesco V,Jaffard R,Higueret P

更新日期：1997-08-04 00:00:00

abstract：:The cytochrome P-450 content of rat hepatocytes declined rapidly over 72 h in culture, due primarily to denaturation to cytochrome P-420. Six different media were investigated for their ability to conserve cytochrome P-450 during culture, and the most successful was a modified Earle's medium. After 72 h culture in thi...

journal_title：FEBS letters

authors： Grant MH,Melvin MA,Shaw P,Melvin WT,Burke MD

更新日期：1985-10-07 00:00:00

abstract：:The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidoge...

journal_title：FEBS letters

authors： Imayavaramban L,Dhayaparan D,Devaraj H

更新日期：2007-11-13 00:00:00

abstract：:At least two of the introns in the antithrombin-III (AT-III) gene are located in positions different from those of the other three proteins in this superfamily for which the gene structures are known, namely, ovalbumin, alpha 1-antitrypsin and angiotensinogen. In another part of the 3'-portion of the AT-III gene there...

journal_title：FEBS letters

authors： Jagd S,Vibe-Pedersen K,Magnusson S

更新日期：1985-12-02 00:00:00

abstract：:Excessive βAR stimulation is an independent factor in inducing pathological cardiac hypertrophy. Here, we report miR-145 regulates both expression and localization of GATA6, thereby protecting the heart against cardiomyocyte hypertrophy induced by isoproterenol (ISO). The protective activity of miR-145 was associated ...

journal_title：FEBS letters

authors： Li R,Yan G,Zhang Q,Jiang Y,Sun H,Hu Y,Sun J,Xu B

更新日期：2013-06-19 00:00:00

abstract：:Magainins are a family of antimicrobial peptides present in the skin extracts of Xenopus laevis. Both magainin-1 and -2 do not have any significant effect on the activity of protein kinase C (PKC). Magainin-2 was found to be readily phosphorylated by PKC to 0.5 mol 32P/mol of peptide. Neither magainin-1, which has a s...

journal_title：FEBS letters

authors： Nakabayashi H,Brown JH,Morell JL,Chen HC,Huang KP

更新日期：1990-07-02 00:00:00

abstract：:Analysis of nascent gamma B-crystallin peptides accumulating during in vitro translation in a rabbit reticulocyte lysate cell-free system was carried out. As a consequence of the irregular distribution of rare codons along the polypeptide chain of gamma B-crystallin, translation of the two-domain protein is a non-unif...

journal_title：FEBS letters

authors： Komar AA,Jaenicke R

更新日期：1995-12-04 00:00:00

abstract：:A gene encoding a single-chain antibody fragment directed against digoxin (named 1C10 scFv) was cloned in two expression systems. For this purpose, a new baculovirus transfer cassette fully compatible with the procaryotic pHEN vector was constructed. Baculovirus production led to higher yield than did Escherichia coli...

journal_title：FEBS letters

authors： Lemeulle C,Chardès T,Montavon C,Chaabihi H,Mani JC,Pugnière M,Cerutti M,Devauchelle G,Pau B,Biard-Piechaczyk M

更新日期：1998-02-20 00:00:00

abstract：:In Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitat...

journal_title：FEBS letters

authors： Kung AW,Collison K,Banga JP,McGregor AM

更新日期：1988-05-09 00:00:00

abstract：:We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, ...

journal_title：FEBS letters

authors： Jokiranta TS,Tissari J,Teleman O,Meri S

更新日期：1995-11-27 00:00:00

abstract：:Chaperone-mediated autophagy (CMA) is a lysosomal pathway that participates in the degradation of cytosolic proteins. CMA is activated by starvation and in response to stressors that result in protein damage. The selectivity intrinsic to CMA allows for removal of damaged proteins without disturbing nearby functional o...

journal_title：FEBS letters

authors： Kon M,Cuervo AM

更新日期：2010-04-02 00:00:00

abstract：:Single enzyme molecules display inevitable, stochastic fluctuations in their catalytic activity. In metabolism, for instance, the stochastic activity of individual enzymes is averaged out due to their high copy numbers per single cell. However, many processes inside cells rely on single enzyme activity, such as transc...

journal_title：FEBS letters

authors： Schwabe A,Maarleveld TR,Bruggeman FJ

更新日期：2013-09-02 00:00:00

abstract：:The role of p53 as the "guardian of the genome" in differentiated somatic cells, triggering various biological processes, is well established. Recent studies in the stem cell field have highlighted a profound role of p53 in stem cell biology as well. These studies, combined with basic data obtained 20 years ago, provi...

journal_title：FEBS letters

authors： Aloni-Grinstein R,Shetzer Y,Kaufman T,Rotter V

更新日期：2014-08-19 00:00:00

abstract：:Glycogen storage disease type Ia (GSD-Ia) patients manifest a pro-atherogenic lipid profile but are not at elevated risk for developing atherosclerosis. Serum phospholipid, which correlates positively with the scavenger receptor class B type I (SR-BI)-mediated cholesterol efflux, and apolipoprotein A-IV and E, accepto...

journal_title：FEBS letters

authors： Nguyen AD,Pan CJ,Shieh JJ,Chou JY

更新日期：2005-08-29 00:00:00

abstract：:SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentr...

journal_title：FEBS letters

authors： Kalmes A,Deou J,Clowes AW,Daum G

更新日期：1999-02-05 00:00:00

abstract：:The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...

journal_title：FEBS letters

authors： Hatzopoulos GN,Mueller-Dieckmann J

更新日期：2010-03-05 00:00:00

abstract：:(-)-Epigallocatechin-3-O-gallate (EGCG), a polyphenol in green tea, induces apoptosis in acute myeloid leukemia (AML) cells without affecting normal cells. In this study, we observed that cGMP acts as a cell death mediator of the EGCG-induced anti-AML effect through acid sphingomyelinase activation. EGCG activated the...

journal_title：FEBS letters

authors： Kumazoe M,Kim Y,Bae J,Takai M,Murata M,Suemasu Y,Sugihara K,Yamashita S,Tsukamoto S,Huang Y,Nakahara K,Yamada K,Tachibana H

更新日期：2013-09-17 00:00:00

abstract：:To obtain information on the biological significance of yeast fructose-2,6-bisphosphate 6-phosphatase, kinetic data of the purified enzyme [(1987) Eur. J. Biochem. 164, 27-30] have been measured. Maximal activity was found between pH 6 and 7, the apparent Michaelis constant with fructose 2,6-bisphosphate was 7.2 micro...

journal_title：FEBS letters

authors： Plankert U,Purwin C,Holzer H

更新日期：1988-10-24 00:00:00

abstract：:The myristoylated N-terminal latching to the C-terminal lobe of c-Abl was recently demonstrated to be an important regulatory element for the kinase, playing a role similar to that of the tyrosine-phosphorylated C-terminal tail of c-Src. A potential mechanism for activating c-Abl is the dissociation of the myristoylat...

journal_title：FEBS letters

authors： Zhou HX

更新日期：2003-09-25 00:00:00

abstract：:The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 5...

journal_title：FEBS letters

authors： Petrovskaya LE,Lukashev EP,Chupin VV,Sychev SV,Lyukmanova EN,Kryukova EA,Ziganshin RH,Spirina EV,Rivkina EM,Khatypov RA,Erokhina LG,Gilichinsky DA,Shuvalov VA,Kirpichnikov MP

更新日期：2010-10-08 00:00:00