Histone H5 and H1 cross-reacting material is restricted to erythroid cells in chicken.


:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in culture were examined for H5 protein and H5 mRNA. Histone H5 was detected only in erythroid cells in tissue sections of chicken embryos or adult livers. H5 protein and H5 mRNA were found only in erythroid cells in culture. No cross-reacting proteins were detected in any other tissue or cell line examined.




FEBS letters


Shannon MF,Wigley PL,Wells JR




Has Abstract


1985-07-08 00:00:00












  • Sequence analysis of the cloned streptococcal cell surface antigen I/II.

    abstract::The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutans, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequence...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kelly C,Evans P,Bergmeier L,Lee SF,Progulske-Fox A,Harris AC,Aitken A,Bleiweis AS,Lehner T

    更新日期:1989-11-20 00:00:00

  • Detection of a new mutant alpha-1-antichymotrypsin in patients with occlusive-cerebrovascular disease.

    abstract::A new mutant alpha-1-antichymotrypsin (variant ACT) was found by direct sequencing and PCR-single strand conformation polymorphism (PCR-SSCP). This variant ACT was a point mutation of exon V of ACT, with the substitution of Met by Val. Four out of six individuals with this variant ACT had occlusive-cerebrovascular dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuda M,Sei Y,Yamamura M,Yamamoto M,Shinohara Y

    更新日期:1992-06-08 00:00:00

  • Dab1 stabilizes its interaction with Cin85 by suppressing Cin85 phosphorylation at serine 587.

    abstract::Crk and CrkL adaptors play essential neuronal positioning roles downstream of Reelin-induced Dab1 tyrosine phosphorylation. Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1. Here using mass spectrometry, biochemical and mutational...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bior BK,Ballif BA

    更新日期:2013-01-04 00:00:00

  • Epidermal growth factor stimulates hydrolysis of phosphatidylinositol 4,5-bisphosphate, generation of diacylglycerol and exocytosis in mouse spermatozoa.

    abstract::Mouse spermatozoa stimulated with epidermal growth factor (EGF) or zona pellucida (ZP) experienced phosphatidylinositol 4,5-bisphosphate hydrolysis, diacylglycerol (DAG) generation and acrosomal exocytosis. The agonists showed additive effects but the action of EGF is likely to be mediated by a distinct receptor becau...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murase T,Roldan ER

    更新日期:1995-03-06 00:00:00

  • Crystal structure of the human tau PHF core domain VQIINK complexed with the Fab domain of monoclonal antibody Tau2r3.

    abstract::Neurofibrillary tangles formed by abnormally aggregated tau protein are a histopathological feature of tauopathies. A tau aggregation inhibitor is a potential therapeutic agent for tauopathies. In this study, we prepared a monoclonal antibody for tau, monoclonal antibody to tau protein (Tau2r3), using as epitope the 2...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuchida T,Susa K,Kibiki T,Tsuchiya T,Miyamoto K,In Y,Minoura K,Taniguchi T,Ishida T,Tomoo K

    更新日期:2020-04-13 00:00:00

  • Evidence for Rap1 in vascular smooth muscle cells. Regulation of their expression by platelet-derived growth factor BB.

    abstract::The effect of platelet-derived growth factor (PDGF) on Rap1 expression was investigated in rat vascular smooth muscle cells (SMC). First, evidence for Rap1 proteins was shown by their: (i) detection in membranes using a specific anti-Rap1 antibody, (ii) typical shift in electrophoretic mobility as a consequence of red...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Quarck R,Bryckaert M,Magnier C,Corvazier E,Bredoux R,de Gunzburg J,Fontenay M,Tobelem G,Enouf J

    更新日期:1994-04-04 00:00:00

  • A novel leptin receptor isoform in rat.

    abstract::Five mouse and human leptin receptors (Ob-R) have recently been identified, a long isoform (Ob-Rb), preferentially expressed in hypothalamus, and 4 short isoforms, Ob-Ra, Ob-Rc, Ob-Rd, and Ob-Re. We have identified a new short isoform in the rat, r-OB-Rf, with 6 C-terminal amino acids and a 3' untranslated region with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang MY,Zhou YT,Newgard CB,Unger RH

    更新日期:1996-08-26 00:00:00

  • Converging antigenic structure of a recombinant viral peptide displayed on different frameworks of carrier proteins.

    abstract::A peptide reproducing the G-H loop amino acid sequence of foot-and-mouth disease virus VP1 protein was fused to the solvent-exposed C-terminus of the bacteriophage P22 tailspike protein [Carbonell and Villaverde (1996) Gene, in press], a homotrimeric polypeptide with a strong beta-helical structure. This fusion does n...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carbonell X,Benito A,Villaverde A

    更新日期:1996-11-18 00:00:00

  • The neurotoxin histrionicotoxin interacts with the putative ion channel of the nicotinic acetylcholine receptors in the central nervous system.

    abstract::Perhydrohistrionicotoxin at micromolar concentrations blocked the nicotine-evoked transmitter release from perfused striatal (dopaminergic) and hippocampal (cholinergic) nerve terminals. Perhydrohistrionicotoxin failed to compete with [3H]nicotine for its high-affinity binding site in rat brain, suggesting that the ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rapier C,Wonnacott S,Lunt GG,Albuquerque EX

    更新日期:1987-02-23 00:00:00

  • Knock-out of the cyaY gene in Escherichia coli does not affect cellular iron content and sensitivity to oxidants.

    abstract::Friedreich ataxia is a recessively inherited neurodegenerative disease caused by deficiency of a highly conserved mitochondrial protein, frataxin. Frataxin deficiency results in mitochondrial iron accumulation and oxidative stress. Frataxin shows homology with the CyaY proteins of gamma-purple bacteria, whose function...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li DS,Ohshima K,Jiralerspong S,Bojanowski MW,Pandolfo M

    更新日期:1999-07-30 00:00:00

  • FixK₂, a key regulator in Bradyrhizobium japonicum, is a substrate for the protease ClpAP in vitro.

    abstract::FixK₂ is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP₁ and ClpXP₁ were purified and tested. FixK...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bonnet M,Stegmann M,Maglica Ž,Stiegeler E,Weber-Ban E,Hennecke H,Mesa S

    更新日期:2013-01-04 00:00:00

  • Agarose gel electrophoretic evidence for domains of nuclear DNA linked with bonds cleavable with sulfhydryl molecules.

    abstract::Complexes of intact nuclear DNA with proteins undissociable by 2.0 M NaCl and nonionic detergents were analyzed by agarose gel electrophoresis following physical or enzymatic fragmentation. Sulfhydryl molecules converted these DNAs (but not the bacteriophage lambda DNA) into smaller-Mr forms. Following limited restric...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taş S,De Larco J,Altiok E

    更新日期:1985-10-21 00:00:00

  • Cholesterol reverts Triton X-100 preferential solubilization of sphingomyelin over phosphatidylcholine: a 31P-NMR study.

    abstract::The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quant...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahyayauch H,Collado MI,Goñi FM,Lichtenberg D

    更新日期:2009-09-03 00:00:00

  • TNF-alpha enhances intracellular glucocorticoid availability.

    abstract::For understanding the mechanism(s) relating inflammation to corticosteroid action, the effect of tumour necrosis factor-alpha (TNF-alpha) on 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), the enzyme regulating access of 11beta-hydroxycorticosteroids to receptors, was studied in LLC-PK(1) cells. We observed ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Heiniger CD,Rochat MK,Frey FJ,Frey BM

    更新日期:2001-11-02 00:00:00

  • Cloning and sequence analysis of a cDNA encoding rice glutaredoxin.

    abstract::A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minakuchi K,Yabushita T,Masumura T,Ichihara K,Tanaka K

    更新日期:1994-01-10 00:00:00

  • Altered cleavage site preference of a proteolytic antibody light chain induced by denaturation.

    abstract::A recombinant antibody light chain (L chain) maintained under non-denaturing conditions displayed preferential cleavage of synthetic peptides conjugated to methylcoumarinamide (MCA) on the C-terminal side of Arg and Lys residues. The same L chain renatured from a denaturing solvent (guanidine hydrochloride) acquired t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sun M,Paul S

    更新日期:1997-05-05 00:00:00

  • Evidence for a novel phosphopantetheinyl transferase domain in the polyketide synthase for enediyne biosynthesis.

    abstract::The polyketide synthase associated with the biosynthesis of enediyne-containing calicheamicin contains a putative phosphopantetheinyl transferase (PPTase) domain. By cloning and expressing the C-terminal region of the polyketide synthase and in vitro phosphopantetheinylation assay, we found that the PPTase domain exhi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murugan E,Liang ZX

    更新日期:2008-04-02 00:00:00

  • Enhanced oxidizability of ubiquinol and alpha-tocopherol during lovastatin treatment.

    abstract::A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

    journal_title:FEBS letters

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Palomäki A,Malminiemi K,Metsä-Ketelä T

    更新日期:1997-06-30 00:00:00

  • Somatostatinergic phenotype markers in the human neuroblastoma cell-line LA-N-2.

    abstract::We have characterized somatostatinergic phenotype markers of the human neuroblastoma, LA-N-2. A single mRNA-transcript (approximately 850bp) and two cellular somatostatin immunoreactivity forms, a high molecular weight form (M(r) 15,000) and a fragment corresponding to somatostatin-28 was found, while the somatostatin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nilsson L,Bergström L,Meyersson G,Påhlman S,Winblad B,Folkesson R

    更新日期:1995-09-18 00:00:00

  • Influence of polyamines on DNA binding of heat shock and activator protein 1 transcription factors induced by heat shock.

    abstract::Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Desiderio MA,Dansi P,Tacchini L,Bernelli-Zazzera A

    更新日期:1999-07-16 00:00:00

  • Influenza A virus-induced caspase-3 cleaves the histone deacetylase 6 in infected epithelial cells.

    abstract::Histone deacetylase 6 (HDAC6) is a multi-substrate cytoplasmic enzyme that regulates many important biological processes. Recently, some reports have implicated HDAC6 in viral infection. However, nothing is known about its regulation in virus-infected cells. The data presented here for the first time demonstrate the c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Husain M,Harrod KS

    更新日期:2009-08-06 00:00:00

  • The isolated N-terminal extracellular domain of the glucagon-like peptide-1 (GLP)-1 receptor has intrinsic binding activity.

    abstract::The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wilmen A,Göke B,Göke R

    更新日期:1996-11-25 00:00:00

  • Non-radioactive reverse transcriptase/polymerase chain reaction for quantification of myosin heavy chain mRNA isoforms in various rabbit muscles.

    abstract::A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peuker H,Pette D

    更新日期:1993-03-08 00:00:00

  • Do the major human glutathione S-transferases have fatty acid ethyl ester synthase activity?

    abstract::Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suzuki T,Kovacs MS,Board PG

    更新日期:1990-11-26 00:00:00

  • Identification of amino acid residues of Ras protein that are essential for signal-transducing activity but not for enhancement of GTPase activity by GAP.

    abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fujita-Yoshigaki J,Shirouzu M,Koide H,Nishimura S,Yokoyama S

    更新日期:1991-12-09 00:00:00

  • Organization of rat uricase chromosomal gene differs greatly from that of the corresponding plant gene.

    abstract::The rat chromosomal gene for uricase was cloned. The gene spans more than 20 kilobases and the coding region is divided into 8 exons by 7 introns. The organization of the rat uricase gene is so greatly different from that of the soybean gene that the difference may not have been caused only by the removal of some ance...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Motojima K,Goto S

    更新日期:1990-05-07 00:00:00

  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.

    abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

    更新日期:2003-01-02 00:00:00

  • PKC-independent inhibition of glutamate exocytosis by arachidonic acid in rat cerebrocortical synaptosomes.

    abstract::In rat cerebrocortical synaptosomes, the addition of 4 beta-phorbol dibutyrate (4 beta-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or pre-incubation with staurosporine, pre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Herrero I,Miras-Portugal MT,Sánchez-Prieto J

    更新日期:1992-01-27 00:00:00

  • The glycan core of GPI-anchored proteins modulates aerolysin binding but is not sufficient: the polypeptide moiety is required for the toxin-receptor interaction.

    abstract::Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

    更新日期:2002-02-13 00:00:00

  • Reversible inactivation of endothelial nitric oxide synthase by NG-nitro-L-arginine.

    abstract::NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mayer B,Schmid M,Klatt P,Schmidt K

    更新日期:1993-10-25 00:00:00