Abstract:
:Germ-line mutations in BRCA1 are associated with an increased lifetime risk of developing breast and/or ovarian tumors. The BRCA1 gene product is a 220-kDa protein that contains a tandem of two BRCA1 C-terminal (BRCT) domains required for transcription. In an attempt to understand how BRCA1 exerts its function through BRCT domains, we search for partners of the BRCT domains of BRCA1. Using the yeast two-hybrid system, we identified the four and a half LIM-only protein 2 (FHL2) as a novel BRCA1 interacting protein. We demonstrate that BRCA1 and FHL2 can physically associate in vitro, in yeast, and in human cells. BRCA1 interacted with FHL2 through its second BRCT domain and the interaction of FHL2 with BRCA1 requires the last three LIM domains of FHL2. BRCA1 enhanced FHL2-mediated transcriptional activity in transient transfections. Tumor-derived transactivation-deficient BRCA1 mutants showed a reduced ability to enhance transactivation by FHL2. Lack of BRCA1 binding sites in the FHL2 completely abolished the FHL2 transactivation function. Reverse transcription polymerase chain reaction analysis showed that FHL2 mRNA levels may be downregulated in many breast cancer cell lines. These results suggest that the BRCA1-FHL2 interaction may be involved in transcriptional regulation and play a significant role in cancer cell growth.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yan J,Zhu J,Zhong H,Lu Q,Huang C,Ye Qdoi
10.1016/s0014-5793(03)00978-5keywords:
subject
Has Abstractpub_date
2003-10-09 00:00:00pages
183-9issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579303009785journal_volume
553pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Although initially viewed as housekeeping enzymes, research over the last 15 years has revealed that the protein tyrosine phosphatases (PTPs) are critical regulators of tyrosine phosphorylation-dependent signaling events and may represent novel targets for therapeutic intervention in a variety of human diseases. In th...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(03)00603-3
更新日期:2003-07-03 00:00:00
abstract::We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80360-s
更新日期:1992-02-03 00:00:00
abstract::Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of alpha 2-macroglobulin. Interleukin-1 beta, interleukin-6 and transforming growth factor-beta synergize with hydrocortisone, increasing the synthesis of alpha 2-macroglobulin 2- to 4-fold, althoug...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80483-b
更新日期:1993-10-11 00:00:00
abstract::The serine peptidases, thrombocytin and PA-BJ, isolated from the venom of Bothrops atrox and Bothrops jararaca, respectively, induce platelet aggregation and granule secretion without clotting fibrinogen. The specific platelet aggregation activity of each enzyme was about 15 times lower than that of thrombin. This act...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01803-2
更新日期:2000-07-21 00:00:00
abstract::N-glycosidically-linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were separated by high-voltage electrophoresis. Five fractions were obtained, one of them representing 60% of the total amount of the N-glycosidically-linked glycans of FVIII/vWf. On the basis of the carbo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80334-2
更新日期:1983-01-10 00:00:00
abstract::Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80529-r
更新日期:1990-12-10 00:00:00
abstract::Using crude membrane preparations of Saccharomyces cerevisiae, we have demonstrated that glucose and glucose analogues which are not efficiently phosphorylated activate the guanine nucleotide-dependent adenylate cyclase in vitro. The activation appears to be mediated by the Ras proteins. Moreover, data are presented i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81221-s
更新日期:1991-09-23 00:00:00
abstract::The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80846-3
更新日期:1987-08-17 00:00:00
abstract::The success of Cre-mediated conditional gene targeting in liver of mice has until now depended on the generation of Cre recombinase transgenic mice or on viral-mediated transduction. Here, we sought to establish the feasibility of using hydrodynamic gene delivery of Cre recombinase into liver, using a ROSA26 EGFP mous...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.094
更新日期:2006-08-07 00:00:00
abstract::Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02771-5
更新日期:2001-08-24 00:00:00
abstract::Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81260-8
更新日期:1989-04-10 00:00:00
abstract::Pretreatment of tissues with potassium channel openers (KCO's) has been observed to be cytoprotective in a broad variety of insults. This phenomenon has been proposed to be intimately linked to activation of mitochondrial potassium channels which apparently modulate the mitochondrial production of reactive oxygen spec...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.013
更新日期:2010-05-17 00:00:00
abstract::Piriprost and nordihydroguiaretic acid (NDGA), specific inhibitors of arachidonate lipoxygenase, inhibited phytohaemagglutinin (PHA)-stimulated breakdown of inositol lipids in human T lymphocytes. The dual inhibitors eicosatetraynoic acid (ETYA) and BW 755C, which inhibit both lipoxygenase and cyclooxygenase, also had...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81621-7
更新日期:1989-11-20 00:00:00
abstract::Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02171-8
更新日期:2001-02-23 00:00:00
abstract::Diphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80006-5
更新日期:1985-10-28 00:00:00
abstract::ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated met...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81174-1
更新日期:1985-03-11 00:00:00
abstract::Amino acid sequences of cytotoxin-like basic proteins (CLBPs), purified from the venoms of Formosan cobra (Naja naja atra) and Indian cobra (Naja naja), were reinvestigated. The determined sequences differed from those reported previously by Takechi et al. [(1985) Biochem. Int. 11, 795-802; (1987) Biochem. Int. 14, 14...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81561-3
更新日期:1989-11-06 00:00:00
abstract::The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80203-3
更新日期:1994-01-17 00:00:00
abstract::Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80942-n
更新日期:1993-08-16 00:00:00
abstract::We studied the effect of acrolein, an alpha,beta-unsaturated aldehyde that causes adduct-modification of lysine, cysteine, and histidine residues, on antithrombin, a key anticoagulant serpin. Intrinsic fluorescence, functionality (anti-FXa and anti-IIa activity), heparin affinity and conformational features of plasma ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.07.062
更新日期:2009-10-06 00:00:00
abstract::A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00706-3
更新日期:1994-08-08 00:00:00
abstract::Impaired functioning of the proteasome pathway is one of the molecular mechanism underlying neurodegenerative changes in Alzheimer's disease. In this study, we report that dysfunction of the proteasome pathway in astroglial cells leads to decreased survival and dysregulation of chemokines by differential regulation of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.08.065
更新日期:2007-10-02 00:00:00
abstract::Telomerase is a multimolecular complex of reverse transcriptase, RNA template, and regulatory proteins. It has two known functions: catalysis of the addition of [TTAGGG] repeats to telomeric DNA and the activation of various genes controlling cell proliferation. The possible coordination of these two functions is a ke...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.12.007
更新日期:2007-01-09 00:00:00
abstract::A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00305-0
更新日期:1997-04-21 00:00:00
abstract::Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase bindi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01362-9
更新日期:1999-10-29 00:00:00
abstract::The bi-functional protein dimerization cofactor of HNF1 (DCoH)/pterin-4 alpha-carbinolamine dehydratase (PCD) is found in liver cell nuclei bound to the transcription factor hepatocyte nuclear factor 1 (HNF1) as well as in the cytoplasm acting as an enzyme involved in the phenylalanine hydroxylation system. Deficiency...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01325-u
更新日期:1995-01-02 00:00:00
abstract::The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02561-3
更新日期:2001-06-22 00:00:00
abstract::cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.082
更新日期:2005-08-15 00:00:00
abstract::Exposure of protein kinase C to low concentrations of either N-chlorosuccinimide or H2O2 resulted in rapid and parallel loss of phosphotransferase activity and phorbol ester binding. This oxidative inactivation of protein kinase C also occurred in intact cells exposed to a low concentration of H2O2. With H2O2 treatmen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81164-x
更新日期:1987-12-10 00:00:00
abstract::Lewy bodies (LBs) are the pathologic hallmark of Parkinson's disease. Recent studies revealed that LBs exhibit several morphologic and molecular similarities to aggresomes. Aggresomes are perinuclear aggregates representing intracellular deposits of misfolded proteins. Recently, valosin-containing protein (VCP) was on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.044
更新日期:2006-01-23 00:00:00