Abstract:
:Under conditions of molar excess of enzyme, isolated F1-ATPase from beef heart mitochondria catalyses ATP hydrolysis biphasically. The rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. The slow phase of ATP hydrolysis is insensitive to EDTA. [gamma-32P]ATP splitting in the slow phase cannot be chased from the enzyme during several catalytic turnovers. It follows from these results that the slow single-site hydrolysis of ATP (kcat approximately 10(-4) s-1), initially observed by Grubmeyer et al. [(1982) J. Biol. Chem. 257, 12092-12100], is not carried out by the normal catalytic site. In contrast, the phase of rapid ATP hydrolysis (kcat approximately 10(-1) s-1) is completely prevented by EDTA and is believed to be the normal function of the normal catalytic site of F1-ATPase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Milgrom YaM,Murataliev MBdoi
10.1016/0014-5793(87)80186-2subject
Has Abstractpub_date
1987-09-28 00:00:00pages
32-6issue
1eissn
0014-5793issn
1873-3468journal_volume
222pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The pancreatic ATP-sensitive potassium channels comprise two subunits: SUR1 and Kir6.2. Two SUR1 mutations, A116P and V187D, reduce channel activity causing persistent hyperinsulinemic hypoglycemia of infancy. We investigated whether these mutations cause temperature sensitive misfolding. We show that the processing d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.039
更新日期:2005-08-01 00:00:00
abstract::Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01184-0
更新日期:2003-11-20 00:00:00
abstract::Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01367-8
更新日期:1999-11-12 00:00:00
abstract::A rat urokinase receptor (uPAR) cDNA fragment was amplified by RT-PCR from RNA of the rat osteoblastic cell line CFK-1. Using this DNA species as a hybridization probe two rat uPAR cDNAs were isolated from a CFK-1 cDNA library. These two cDNAs encode an identical uPAR protein except for a single base mutation which re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80118-5
更新日期:1994-01-24 00:00:00
abstract::Placentas of mice lacking p57(Kip2) expression have trophoblastic hyperplasia. To elucidate the mechanism underlying this phenomenon, we studied expression of two angiogenic factors, vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Immunohistochemical analysis with anti-VEGF antibodies indi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03681-5
更新日期:2002-12-18 00:00:00
abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00929-6
更新日期:1998-08-21 00:00:00
abstract::Myasthenia gravis (MG) is caused by autoantibodies to the acetylcholine receptor (AChR). Experiments with fetal (alpha(2)betagammadelta) and adult (alpha(2)betaepsilondelta) AChR and with recombinant subunit dimers showed that some monoclonal antibodies (mAbs) against the main immunogenic region (MIR), located on the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01980-3
更新日期:2000-09-15 00:00:00
abstract::An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02615-1
更新日期:2001-07-06 00:00:00
abstract::Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.07.025
更新日期:2009-09-03 00:00:00
abstract::Tyrosine O-sulfation is one of the post-translational modification processes that occur to membrane proteins and secreted proteins in eukaryotes. Tyrosylprotein sulfotransferase (TPST) is responsible for this modification, and in this report, we describe the expression pattern and the biological role of TPST-A in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.044
更新日期:2005-01-03 00:00:00
abstract::In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.012
更新日期:2006-04-03 00:00:00
abstract::Plants have evolved to cope with fluctuations in water supply by gating their water channels known as aquaporins. During flooding, a rapid drop of cytosolic pH due to anoxia leads to a simultaneous closure of the aquaporins in the plasma membrane. The closing mechanism has been suggested to involve a conserved histidi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.02.038
更新日期:2013-04-02 00:00:00
abstract::The tissue inhibitors of matrix metalloproteinases (MMPs), TIMP-1 and TIMP-2, are also angiogenesis inhibitors. Cathepsin B and MMPs are found at sites of neovascularization in pathologies such as cancer and osteoarthritis. Treatment of TIMP-1, TIMP-2, and of a mixture of both inhibitors from human articular chondrocy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00897-2
更新日期:1999-07-23 00:00:00
abstract::Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00626-1
更新日期:1997-06-30 00:00:00
abstract::The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.044
更新日期:2006-11-27 00:00:00
abstract::We searched for the previously defined uncoupling protein (UCP) signatures [Jezek, P. and Urbánková, E. (2000) IUBMB Life 49, 63-70] in genomes of Drosophila melanogaster, Caenorhabditis elegans, Dictyostelium discoideum, and Arabidopsis thaliana. We identified four UCPs in Drosophila and one in Caenorhabditis or Dict...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02338-9
更新日期:2001-04-27 00:00:00
abstract::We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81761-n
更新日期:1993-07-12 00:00:00
abstract::Tropomyosins can be classified as belonging to an alpha-like or a beta-like family depending on the absence or presence, respectively in their protease-V8 digestion pattern of two peptides with an apparent molecular mass of 21 kDa. Chicken cardiac tropomyosin and the 43 kDa component from gizzard tropomyosin are accor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80829-1
更新日期:1983-11-14 00:00:00
abstract::Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.050
更新日期:2013-09-17 00:00:00
abstract::We show that N-1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S1-nuclease treatment of chromatin and chromosomes. This suggests a disa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80167-7
更新日期:1983-04-18 00:00:00
abstract::E. coli trigger factor is an abundant cytosolic protein originally identified by its ability to maintain the precursor of a secretory protein in a translocation competent form. Recent studies shed new light on the function of this protein. Trigger factor was found to be a peptidyl-prolyl-cisltrans-isomerase capable of...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(96)00582-0
更新日期:1996-06-24 00:00:00
abstract::NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80405-j
更新日期:1993-10-25 00:00:00
abstract::On the basis of recent results, we propose a model for DNA polymerase translocation along DNA. Human immunodeficiency virus reverse transcriptase is taken as an example. According to the model, movement of the enzyme is the result of transition of the enzyme-bound DNA from the A- to B-form which is accompanied by leng...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(96)00479-6
更新日期:1996-07-15 00:00:00
abstract::SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00034-4
更新日期:1999-02-05 00:00:00
abstract::The site-specific recombination system of bacteriophage P1 is composed of the Cre recombinase that recognizes a 34-bp loxP site. The Cre/loxP system has been extensively used to manipulate eukaryotic genomes for functional genomic investigations. The creation of additional heterologous loxP sequences potentially expan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02541-8
更新日期:2001-06-15 00:00:00
abstract::It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(69)80028-1
更新日期:1969-02-01 00:00:00
abstract::Single enzyme molecules display inevitable, stochastic fluctuations in their catalytic activity. In metabolism, for instance, the stochastic activity of individual enzymes is averaged out due to their high copy numbers per single cell. However, many processes inside cells rely on single enzyme activity, such as transc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.005
更新日期:2013-09-02 00:00:00
abstract::Purified barley leaf ADP-glucose pyrophosphorylase, a key enzyme of the starch synthesis in the chloroplast stroma, was analysed with respect to its possible regulation by factors defining the metabolic/effector status of the chloroplast during light and dark conditions. The enzyme required 3-phosphoglyceric acid for ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00346-4
更新日期:1999-04-01 00:00:00
abstract::Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products, Ins 1,2 greater than P and Ins 1P. Use of these methods reveals that the two iso-enzymes liberate, with a high degree of reprod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81380-1
更新日期:1986-08-11 00:00:00
abstract::Choroidal melanoma (CM) remains the most prevalent form of intraocular malignancy, and the prognosis of affected patients is poor. While the E3 ubiquitin ligase TRAF-interacting protein (TRIP) is known to play key regulatory roles in multiple diseases, its relevance in CM remains uncertain. In the present study, we fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13882
更新日期:2020-10-01 00:00:00