Abstract:
:Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic cleft of protein kinases and is therefore least probable to be a target for any modification. This communication illustrates the accessibility of the gatekeeper site (Y670) towards both autophosphorylation and dephosphorylation in the recombinant cytoplasmic domain of symbiosis receptor kinase from Arachis hypogaea (AhSYMRK). Autophosphorylation on gatekeeper tyrosine was detected prior to extraction but never under in vitro conditions. We hypothesize gatekeeper phosphorylation to be associated with synthesis/maturation of AhSYMRK and this phenomenon may be prevalent among RLKs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Samaddar S,Dutta A,Sinharoy S,Paul A,Bhattacharya A,Saha S,Chien KY,Goshe MB,DasGupta Mdoi
10.1016/j.febslet.2013.07.050subject
Has Abstractpub_date
2013-09-17 00:00:00pages
2972-9issue
18eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00615-7journal_volume
587pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81015-7
更新日期:1984-07-23 00:00:00
abstract::Abnormal retention of Delta F508 CFTR (cystic fibrosis transmembrane conductance regulator) in the endoplasmic reticulum is a major cause of cystic fibrosis (CF). We show that calnexin Delta 185-520 but not calnexin can partially reverse the mislocalization of Delta F508 CFTR. This 256-amino acid protein has neither t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03134-4
更新日期:2002-08-28 00:00:00
abstract::The Bacillus thuringiensis Cry toxin causes swelling and necrosis in insect cells, but the route(s) and significance of the water influx involved in its cytotoxicity are unclear. Here, we assessed the role of aquaporins (AQPs), known as water channels, in Cry toxin intoxication. An AQP inhibitor did not interfere with...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12506
更新日期:2017-01-01 00:00:00
abstract::We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01599-4
更新日期:2000-06-02 00:00:00
abstract::(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O(2) levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.07.065
更新日期:2012-09-21 00:00:00
abstract::Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ-diabetic rats using N...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01506-3
更新日期:1998-12-04 00:00:00
abstract::The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80275-8
更新日期:1988-08-15 00:00:00
abstract::RKIP was first identified as an inhibitor of the Raf-MEK-ERK signaling pathway. RKIP was also found to play an important role in the NF-kappaB pathway. Genetic and biochemical studies demonstrated that RKIP functioned as a scaffold protein facilitating the phosphorylation of IkappaB by upstream kinases. However, contr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.12.051
更新日期:2010-02-19 00:00:00
abstract::Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01683-4
更新日期:1999-01-25 00:00:00
abstract::Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80194-1
更新日期:1987-09-28 00:00:00
abstract::Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.09.051
更新日期:2006-10-30 00:00:00
abstract::We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00497-4
更新日期:1999-05-07 00:00:00
abstract::The interaction of the P-beta-Cat(19-44) peptide, a 26 amino acid peptide (K(19)AAVSHWQQQSYLDpSGIHpSGATTTAP(44)) that mimics the phosphorylated beta-Catenin antigen, has been studied with its monoclonal antibody BC-22, by transferred nuclear Overhauser effect NMR spectroscopy (TRNOESY) and saturation transfer differen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.084
更新日期:2006-10-02 00:00:00
abstract::Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGF-β) family that signal via type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors. BMPs are multifunctional regulators of development and tissue homeostasis and they were initially characte...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.04.030
更新日期:2012-07-04 00:00:00
abstract::Gene conservation, duplication and constitutive expression are intricately linked and strong predictors of essentiality. Here, we introduce metrics based on diversity indices to measure gene conservation, duplication and constitutive expression and validate them by measuring their performance in prediction of essentia...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12192
更新日期:2016-05-01 00:00:00
abstract::Agonist-induced changes in expression and mRNA levels of luteinizing hormone (LH) receptors were compared during stimulation of ovarian follicular maturation and luteinization by gonadotropic hormones. Three major species of LH receptor mRNA, 5.8, 2.6 and 2.3 kb, were present throughout differentiation and changed sim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81359-v
更新日期:1990-11-12 00:00:00
abstract::Proteins may undergo adaptive structural transitions to accommodate to their cellular milieu and respond to external signals. Modulation of conformational ensembles can rewire the intra- or intermolecular interaction networks and shift between different functional states. Adaptive conformational transitions are associ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12762
更新日期:2017-09-01 00:00:00
abstract::Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01177-1
更新日期:1999-09-24 00:00:00
abstract::The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00830-3
更新日期:1995-08-21 00:00:00
abstract::Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13679
更新日期:2020-03-01 00:00:00
abstract::Trefoil factor family (TFF)-domain peptides 1-3 are mucin-associated molecules, largely found in epithelia of gastrointestinal tissues. Structurally similar, resistant to enzymatic degradation, they are up-regulated around areas of epithelial damage such as ulcers. Transgenic expression or exogenous peptide ameliorate...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(97)00424-9
更新日期:1997-05-19 00:00:00
abstract::Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81016-h
更新日期:1990-07-30 00:00:00
abstract::The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80187-3
更新日期:1985-01-01 00:00:00
abstract::Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.043
更新日期:2014-05-02 00:00:00
abstract::vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles. In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00563-2
更新日期:1997-06-16 00:00:00
abstract::In the present study we show the existence of a functional nitric oxide synthase (NOS) in rat liver mitochondria. The enzyme uses L-arginine (L-arg) to produce nitric oxide (NO) and L-citrulline, and is Ca2+-dependent. L-Arg analogues, N(omega)monomethyl-L-arg and N(omega)-nitro-L-arg, inhibit the enzyme, and D-argini...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01397-5
更新日期:1997-12-01 00:00:00
abstract::Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.016
更新日期:2005-10-31 00:00:00
abstract::We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80510-a
更新日期:1991-05-06 00:00:00
abstract::The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80528-3
更新日期:1988-10-10 00:00:00
abstract::The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80148-6
更新日期:1989-01-30 00:00:00