Abstract:
:vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles. In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps along the translocation and recycling pathways of GLUT4 in the absence and presence of insulin. Western blots of subcellular membrane fractions demonstrate very similar distributions of vp165 and GLUT4. Confocal microscopy of whole cells provides direct evidence that these proteins share the same vesicle populations moving both towards and from the plasma membrane. These data are consistent with the presence of a distinct insulin-sensitive compartment that sequesters both GLUT4 and vp165 and suggest similar trafficking routes through the recycling compartments.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Malide D,St-Denis JF,Keller SR,Cushman SWdoi
10.1016/s0014-5793(97)00563-2subject
Has Abstractpub_date
1997-06-16 00:00:00pages
461-8issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00563-2journal_volume
409pub_type
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