Activation of human pepsinogens.

abstract：:The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in det...

journal_title：FEBS letters

authors： Arseniev AS,Barsukov IL,Bystrov VF,Lomize AL,Ovchinnikov YuA

更新日期：1985-07-08 00:00:00

abstract：:The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic r...

journal_title：FEBS letters

authors： Tsunoda Y,Yodozawa S,Tashiro Y

更新日期：1988-04-11 00:00:00

abstract：:The regulation of genes for acetylcholine receptor (AChR), myogenic factors and other muscle-specific proteins has been analyzed in experimental autoimmune myasthenia gravis (EAMG) and following denervation. The levels of the transcripts for the myogenic factors, MyoD1, myogenin and MRF4, were measured using Northern ...

journal_title：FEBS letters

authors： Asher O,Fuchs S,Zuk D,Rapaport D,Buonanno A

更新日期：1992-03-24 00:00:00

abstract：:DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...

journal_title：FEBS letters

authors： Wang Y,Rea T,Bian J,Gray S,Sun Y

更新日期：1999-02-26 00:00:00

abstract：:The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues...

journal_title：FEBS letters

authors： Pearl EJ,Tyndall JD,Poulter RT,Wilbanks SM

更新日期：2007-06-26 00:00:00

abstract：:The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carbox...

journal_title：FEBS letters

authors： Qin BY,Creamer LK,Baker EN,Jameson GB

更新日期：1998-11-06 00:00:00

abstract：:Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

journal_title：FEBS letters

authors： Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

更新日期：2012-02-17 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactio...

journal_title：FEBS letters

authors： Schuhmacher MK,Kudithipudi S,Jeltsch A

更新日期：2016-06-01 00:00:00

abstract：:Foot-and-mouth disease virus leader proteinase (L(pro)), a papain-like cysteine proteinase, has six acidic amino acids between 4 A and 11 A of the catalytic dyad of Cys51 and His148. In contrast, in papain and related enzymes, only one acidic residue lies within this distance. We have examined by site-directed mutagen...

journal_title：FEBS letters

authors： Kronovetr J,Skern T

更新日期：2002-09-25 00:00:00

abstract：:Niemann-pick type C1 (NPC1) is an endo/lysosomal membrane protein involved in intracellular cholesterol trafficking, and its luminal domain C is an essential endosomal receptor for Ebola and Marburg viruses. We have determined the crystal structure of glycosylated NPC1 luminal domain C and find all seven possible site...

journal_title：FEBS letters

authors： Zhao Y,Ren J,Harlos K,Stuart DI

更新日期：2016-03-01 00:00:00

abstract：:The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT-Raman spectroscopy in reaction centres purified from an antenna-deficient strain of Rhodobacter sphaeroides. We find that mutation at the M210 position does not significantly perturb th...

journal_title：FEBS letters

authors： Jones MR,Heer-Dawson M,Mattioli TA,Hunter CN,Robert B

更新日期：1994-02-14 00:00:00

abstract：:In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...

journal_title：FEBS letters

authors： Fox MT,Harriott P,Walker B,Stone SR

更新日期：1997-11-17 00:00:00

abstract：:The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V-->E at position 2, known to block gp41 activity in vivo, makes the peptide un...

journal_title：FEBS letters

authors： Pereira FB,Goñi FM,Nieva JL

更新日期：1995-04-03 00:00:00

abstract：:The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated beta-(1-->6)-linked D-glucosamine ...

journal_title：FEBS letters

authors： Thérisod H,Monteiro MA,Perry MB,Caroff M

更新日期：2001-06-15 00:00:00

abstract：:Spin-trapping techniques and electron spin resonance (ESR) spectroscopy were used to study the relationship between the effect of streptozotocin (STZ) on pancreatic beta-cells and free radical formation by these cells. Results showed that STZ enhanced generation of the DMPO-OH radical adduct, which is a degradation pr...

journal_title：FEBS letters

authors： Nukatsuka M,Sakurai H,Yoshimura Y,Nishida M,Kawada J

更新日期：1988-11-07 00:00:00

abstract：:Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine ...

journal_title：FEBS letters

authors： Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJ

更新日期：1994-09-12 00:00:00

abstract：:The present study investigated the mechanism by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) inhibit platelet activation induced by thromboxane A2. DHA was found to be more potent than EPA in blocking platelet aggregation induced by the stable thromboxane A2 mimetic, U46619. Furthermore, this inhib...

journal_title：FEBS letters

authors： Swann PG,Venton DL,Le Breton GC

更新日期：1989-01-30 00:00:00

abstract：:We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, ...

journal_title：FEBS letters

authors： Jokiranta TS,Tissari J,Teleman O,Meri S

更新日期：1995-11-27 00:00:00

abstract：:Barth syndrome (BTHS) is a rare X-linked genetic disorder caused by mutations in the gene encoding the transacylase tafazzin and characterized by loss of cardiolipin and severe cardiomyopathy. Mitochondrial oxidants have been implicated in the cardiomyopathy in BTHS. Eleven mitochondrial sites produce superoxide/hydro...

journal_title：FEBS letters

authors： Goncalves RLS,Schlame M,Bartelt A,Brand MD,Hotamışlıgil GS

更新日期：2020-10-28 00:00:00

abstract：:The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...

journal_title：FEBS letters

authors： Bömer U,Pfanner N,Dietmeier K

更新日期：1996-03-11 00:00:00

abstract：:To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c-GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c-GFP is efficiently localised to mitochondria and able to function as an el...

journal_title：FEBS letters

authors： Roucou X,Prescott M,Devenish RJ,Nagley P

更新日期：2000-04-14 00:00:00

abstract：:Bleomycin-reactive iron was detected in the sera of six out of nine adults undergoing intensive chemotherapy for acute non-lymphocytic leukemia. In these individuals the corresponding transferrin saturation ranged from 96% to 113% and the serum ferritin from 775 to 9975 micrograms/l. Nontransferrin-bound iron has been...

journal_title：FEBS letters

authors： Gordeuk VR,Brittenham GM

更新日期：1992-08-10 00:00:00

abstract：:Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

journal_title：FEBS letters

authors： Klip A,Ramlal T,Young DA,Holloszy JO

更新日期：1987-11-16 00:00:00

abstract：:We have previously shown that topoisomerase I (topo I) antagonist inhibited retrovirus replication. Since tyrphostins, synthetic compounds and protein tyrosine kinases (PTKs) blockers, inhibited topo I activity (manuscript in preparation) we examined their ability to inhibit Moloney murine leukemia virus (Mo-MuLV) rep...

journal_title：FEBS letters

authors： Esther A,Iftach S,Esther P

更新日期：1994-03-14 00:00:00

abstract：:The gas phase of the soil plays an important role in plant growth and development. We investigated the effect of rhizospheric NO as a signalling compound for N uptake of beech roots. Following exposure to NO, ammonium and glutamine uptake into roots were determined using (15)N-labelling, and gene expression of selecte...

journal_title：FEBS letters

authors： Simon J,Stoelken G,Rienks M,Rennenberg H

更新日期：2009-09-03 00:00:00

abstract：:The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...

journal_title：FEBS letters

authors： Hatzopoulos GN,Mueller-Dieckmann J

更新日期：2010-03-05 00:00:00

abstract：:After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...

journal_title：FEBS letters

authors： Chan JS,Nie ZR,Seidah NG,Chrétien M

更新日期：1986-04-21 00:00:00

abstract：:Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...

journal_title：FEBS letters

authors： Michot B,Despres L,Bonhomme F,Bachellerie JP

更新日期：1993-02-01 00:00:00

abstract：:The somatostatin upstream enhancer (SMS-UE) is a highly complex enhancer element. The distal A-element contains overlapping Pdx1 and Pbx binding sites. However, a point mutation in the A-element that abolishes both Pdxl and Pbx binding does not impair promoter activity. In contrast, a point mutation that selectively e...

journal_title：FEBS letters

authors： Andersen FG,Jensen J,Heller RS,Petersen HV,Larsson LI,Madsen OD,Serup P

更新日期：1999-02-26 00:00:00