Abstract:
:Foot-and-mouth disease virus leader proteinase (L(pro)), a papain-like cysteine proteinase, has six acidic amino acids between 4 A and 11 A of the catalytic dyad of Cys51 and His148. In contrast, in papain and related enzymes, only one acidic residue lies within this distance. We have examined by site-directed mutagenesis the importance of each of these residues for L(pro) self-processing and cleavage of its cellular substrate, eukaryotic initiation factor 4GI. Only substitution of the electrostatic charge of aspartate 164 affected enzyme activity. Thus, in contrast to the prototype papain, L(pro) activity requires a negative charge 4.5 A from the catalytic dyad.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kronovetr J,Skern Tdoi
10.1016/s0014-5793(02)03237-4keywords:
subject
Has Abstractpub_date
2002-09-25 00:00:00pages
58-62issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302032374journal_volume
528pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13683
更新日期:2020-03-01 00:00:00
abstract::FixK₂ is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP₁ and ClpXP₁ were purified and tested. FixK...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.11.014
更新日期:2013-01-04 00:00:00
abstract::We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to inter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.054
更新日期:2008-06-11 00:00:00
abstract::Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80715-7
更新日期:1993-11-22 00:00:00
abstract::Modelling the amino acid sequence of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase into the three-dimensional structure of 3 alpha/20 beta-hydroxysteroid dehydrogenase shows that these two enzymes, as well as the NADP(+)-linked prostaglandin dehydrogenase (identical to carbonyl reductase) have similar conformati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81554-d
更新日期:1993-05-10 00:00:00
abstract::Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00144-6
更新日期:1998-03-06 00:00:00
abstract::Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80238-c
更新日期:1992-03-09 00:00:00
abstract::A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80213-3
更新日期:1989-03-13 00:00:00
abstract::Peroxynitrite (ONOO-) has recently been implicated in connective tissue destruction in vivo. We have studied the effect of ONOO- on the activity of tissue inhibitor of metalloproteinase-1 (TIMP-1) in vitro. The inactivation of TIMP-1 by ONOO- was dose dependent with 50 microM ONOO- reducing the inhibitory activity of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00065-8
更新日期:1996-02-26 00:00:00
abstract::Dystrophin is the recently discovered defective gene product in Duchenne and Becker muscular dystrophy (DMD and BMD). Dystrophin transcripts have been amplified and identified in diagnostic needle muscle biopsy samples using the polymerase chain reaction (PCR) procedure. Using 5'- and 3'-primers, dystrophin transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80896-8
更新日期:1989-07-31 00:00:00
abstract::The full-length cDNA encoding the human calbindin-D9k (CaBP-9k) has been cloned using reverse transcription/PCR methodology with rat- and bovine-derived primers and intestinal RNA. A core product, and both a 5' and 3' product encompassing the full-length cDNA were obtained. The clones include coding region for 79 amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80772-9
更新日期:1992-07-28 00:00:00
abstract::Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.060
更新日期:2007-04-03 00:00:00
abstract::Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.035
更新日期:2010-11-19 00:00:00
abstract::Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00386-6
更新日期:1994-05-16 00:00:00
abstract::Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.07.039
更新日期:2014-10-16 00:00:00
abstract::Carboxypeptidase H (CPH) is one of several processing enzymes required for the conversion of peptide hormone precursors into their smaller active forms. In this study, high levels of CPH activity was found in a liver metastasis of a human ileal carcinoid which expresses beta-preprotachykinin mRNA and the tachykinin ne...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80508-8
更新日期:1988-10-10 00:00:00
abstract::We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80510-a
更新日期:1991-05-06 00:00:00
abstract::c-Myc is a transcriptional regulator involved in carcinogenesis through its role in growth control and cell cycle progression. Here we attempt to relate its role in stimulating the G1-S transition to the ability to affect functioning of key cell cycle regulators, and we focus on how its property of modulating transcri...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02118-4
更新日期:2001-02-16 00:00:00
abstract::A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensiona...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00473-m
更新日期:1995-05-29 00:00:00
abstract::The interaction of vinculin with actin filaments was investigated by methods which exclude interference by contaminating proteins which may occur in vinculin preparations. Vinculin which was blotted from SDS-polyacrylamide gels onto nitrocellulose, was stained specifically by fluorescently labeled polymeric actin (100...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80595-7
更新日期:1988-02-08 00:00:00
abstract::Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01455-a
更新日期:1995-01-30 00:00:00
abstract::Horseradish peroxidase in the presence of hydrogen peroxide (HRP/H2O2) oxidizes a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxynaphthalene (Sudan I) to the ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the HRP/H2O2 system is the benzenediazonium ion. Minor pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80776-2
更新日期:1988-05-23 00:00:00
abstract::Barley thioredoxin h isozymes 1 (HvTrxh1) and barley thioredoxin h isozymes 2 (HvTrxh2) show distinct spatiotemporal distribution in germinating seeds. Using a novel approach involving measurement of bidirectional electron transfer rates between Escherichia coli thioredoxin, which exhibits redox-dependent fluorescence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.028
更新日期:2010-08-04 00:00:00
abstract::There is strong evidence of a key role for mitochondrial dysfunction in both sporadic and all forms of familial Parkinson's disease (PD). However, none of the clinical trials carried out with putative mitochondrial rescue agents have been successful. Firm establishment of a wet biomarker or a reliable readout from ima...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12920
更新日期:2018-03-01 00:00:00
abstract::In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.050
更新日期:2012-04-05 00:00:00
abstract::The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.11.013
更新日期:2013-01-16 00:00:00
abstract::A rapid and efficient method for the purification of malate synthase, an enzyme uniquely confined to glyoxysomes, from cotyledons of Brassica napus L. has been developed. The two step purification procedure is based on the consequent utilization of the tendency of malate synthase to form high molecular weight aggregat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01114-t
更新日期:1995-10-30 00:00:00
abstract::Post-absorptive gluconeogenesis from lactate measured in vivo increases 3-fold in 24-month-old rats compared to 3-month-old animals. Fractional lactate turnover rates showed no significant differences between the two groups of animals. Lower plasma glucose concentrations and insulin-glucagon ratios may explain the inc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80318-0
更新日期:1986-03-03 00:00:00
abstract::Sulfatide, which binds to influenza A viruses and prevents the viral infection, was found to inhibit the sialidase activities of influenza A viruses in a pH-dependent manner. The kinetic parameters of the effect of sulfatide on the sialidase activities of human influenza A viruses using fluorometric assay indicated th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01045-7
更新日期:2003-10-23 00:00:00
abstract::Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01177-1
更新日期:1999-09-24 00:00:00