Abstract:
:FixK₂ is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP₁ and ClpXP₁ were purified and tested. FixK₂ was found to be degraded by ClpAP₁ but not by ClpXP₁. Degradation was inhibited by the ClpS₁ adaptor protein, indicating that FixK₂ is a direct substrate for ClpAP₁. The last 12 amino acids of FixK₂ appeared to be recognized by ClpA. The results suggest that the ClpAP system is involved in the cellular turnover of FixK₂.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bonnet M,Stegmann M,Maglica Ž,Stiegeler E,Weber-Ban E,Hennecke H,Mesa Sdoi
10.1016/j.febslet.2012.11.014subject
Has Abstractpub_date
2013-01-04 00:00:00pages
88-93issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00873-3journal_volume
587pub_type
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