Abstract:
:Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spontaneous activity. Hormonal stimulations of calcium influx (detected by fura-2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A2, all known to cause phospholipase C-beta activation in mammalian cells. Although the identity of the G-proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G-protein regulated cation channel.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Harteneck C,Obukhov AG,Zobel A,Kalkbrenner F,Schultz Gdoi
10.1016/0014-5793(94)01455-asubject
Has Abstractpub_date
1995-01-30 00:00:00pages
297-300issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)01455-Ajournal_volume
358pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80614-7
更新日期:1986-06-09 00:00:00
abstract::The primary structure of rhodopsin from the octopus Paroctopus defleini has been determined by parallel analysis of the protein and corresponding cDNA. The amino acid sequence is most similar to the recently cloned Drosophila opsins. Similarities to bovine and human opsins are also evident. The transmembrane topology ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80388-0
更新日期:1988-05-09 00:00:00
abstract::LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.020
更新日期:2015-10-07 00:00:00
abstract::Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.10.002
更新日期:2011-11-04 00:00:00
abstract::Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.12.046
更新日期:2005-01-31 00:00:00
abstract::PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00708-5
更新日期:1999-06-18 00:00:00
abstract::Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA. Glycosylation of the enzymes does not significantly affect their kinetic properties, or their stabilities towards heat and pH. However, the glycosylated enzymes ar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81150-x
更新日期:1987-12-10 00:00:00
abstract::Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80661-y
更新日期:1992-07-06 00:00:00
abstract::Carboxylesterase (EII') from Arthrobacter sp. KI72 has 88% homology to 6-aminohexanoate-dimer hydrolase (EII) and possesses ca. 0.5% of the level of 6-aminohexanoate-linear dimer (Ald)-hydrolytic activity of EII. To study relationship between Ald-hydrolytic and esterolytic activities, random mutations were introduced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.031
更新日期:2006-09-18 00:00:00
abstract::In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.062
更新日期:2007-05-29 00:00:00
abstract::Southern blotting using a 5'-proximal probe of the Saccharomyces cerevisiae CYR1 gene has revealed heterogeneity in laboratory strains. It is demonstrated that strain AB320 contains a Ty1 element inserted in the promoter region of CYR1. The Ty1 orientation suggests that transcription of CYR1 is initiated downstream fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81227-9
更新日期:1987-07-13 00:00:00
abstract::The crystal structure for cce_0566 (171 aa, 19.4 kDa), a DUF269 annotated protein from the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142, was determined to 1.60Å resolution. Cce_0566 is a homodimer with each molecule composed of eight α-helices folded on one side of a three strand anti-parallel β-sheet. Hydrop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.037
更新日期:2012-02-17 00:00:00
abstract::Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00535-4
更新日期:1994-06-27 00:00:00
abstract::The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00830-2
更新日期:1997-08-04 00:00:00
abstract::In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80951-x
更新日期:1991-07-29 00:00:00
abstract::In eukaryotes, members of the Ero1 family control oxidative protein folding in the endoplasmic reticulum (ER). Yeast Ero1p is tightly associated with the ER membrane, despite cleavage of the leader peptide, the only hydrophobic sequence that could mediate lipid insertion. In contrast, human Ero1-Lalpha and a yeast mut...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03034-4
更新日期:2001-11-09 00:00:00
abstract::Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.061
更新日期:2005-10-10 00:00:00
abstract::Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.031
更新日期:2008-07-23 00:00:00
abstract::The multidrug resistance protein (MRP) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. In this study we report that dinitrophenyl-S-glutathione increases ATPase activity in plasma membrane vesicles prepared from the MRP-overexpressing cell line GLC4...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00940-x
更新日期:1997-08-18 00:00:00
abstract::Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extract...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81663-1
更新日期:1989-12-04 00:00:00
abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01015-1
更新日期:1998-09-04 00:00:00
abstract::The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02639-4
更新日期:2001-08-03 00:00:00
abstract::Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column dig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01513-2
更新日期:1996-02-05 00:00:00
abstract::The covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is a viral center molecule for HBV infection and persistence. However, the cellular restriction factors of HBV cccDNA are not well understood. Here, we show that TGF-β can induce nuclear viral cccDNA degradation and hypermutation via activation-ind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12058
更新日期:2016-02-01 00:00:00
abstract::The 5'-flanking sequences of the human macrophage inflammatory protein-3alpha/CCL20 gene were cloned and transfected into G-361 human melanoma cells in a luciferase reporter construct. Tumor necrosis factor-alpha (TNF-alpha) treatment stimulated luciferase expression, and promoter truncations demonstrated that TNF-alp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03138-6
更新日期:2001-12-14 00:00:00
abstract::The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.07.010
更新日期:2014-08-25 00:00:00
abstract::Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.060
更新日期:2007-04-03 00:00:00
abstract::In order to better define the structural elements involved in allosteric signalling, wild-type DnaK and three deletion mutants of the peptide binding domain have been characterized by biophysical (steady-state and time-resolved fluorescence) and biochemical methods. In the presence of ATP the chemical environment of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03752-3
更新日期:2003-01-02 00:00:00
abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81273-0
更新日期:1988-08-01 00:00:00
abstract::Coagulation factor Va is an essential cofactor which combines with the serine protease factor Xa on a phospholipid surface to form the prothrombinase complex. In the present study, the structure of factor Va interacting with lipid surfaces containing phosphatidylserine was studied by electron microscopy. Two-dimension...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00881-7
更新日期:1994-09-12 00:00:00