The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors.

Abstract:

:Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spontaneous activity. Hormonal stimulations of calcium influx (detected by fura-2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A2, all known to cause phospholipase C-beta activation in mammalian cells. Although the identity of the G-proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G-protein regulated cation channel.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Harteneck C,Obukhov AG,Zobel A,Kalkbrenner F,Schultz G

doi

10.1016/0014-5793(94)01455-a

subject

Has Abstract

pub_date

1995-01-30 00:00:00

pages

297-300

issue

3

eissn

0014-5793

issn

1873-3468

pii

0014-5793(94)01455-A

journal_volume

358

pub_type

杂志文章
  • TLC immunostaining characterization of Clostridium botulinum type A neurotoxin binding to gangliosides and free fatty acids.

    abstract::The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphat...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80614-7

    authors: Takamizawa K,Iwamori M,Kozaki S,Sakaguchi G,Tanaka R,Takayama H,Nagai Y

    更新日期:1986-06-09 00:00:00

  • Octopus rhodopsin. Amino acid sequence deduced from cDNA.

    abstract::The primary structure of rhodopsin from the octopus Paroctopus defleini has been determined by parallel analysis of the protein and corresponding cDNA. The amino acid sequence is most similar to the recently cloned Drosophila opsins. Similarities to bovine and human opsins are also evident. The transmembrane topology ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80388-0

    authors: Ovchinnikov YuA,Abdulaev NG,Zolotarev AS,Artamonov ID,Bespalov IA,Dergachev AE,Tsuda M

    更新日期:1988-05-09 00:00:00

  • Double-negative feedback loop between long non-coding RNA TUG1 and miR-145 promotes epithelial to mesenchymal transition and radioresistance in human bladder cancer cells.

    abstract::LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2015.08.020

    authors: Tan J,Qiu K,Li M,Liang Y

    更新日期:2015-10-07 00:00:00

  • Effects of a novel peptide derived from human thrombomodulin on endotoxin-induced uveitis in vitro and in vivo.

    abstract::Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2011.10.002

    authors: Jin H,Yang X,Liu K,Gu Q,Xu X

    更新日期:2011-11-04 00:00:00

  • Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery.

    abstract::Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.12.046

    authors: Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

    更新日期:2005-01-31 00:00:00

  • Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal.

    abstract::PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00708-5

    authors: Wang Z,Takemori H,Halder SK,Nonaka Y,Okamoto M

    更新日期:1999-06-18 00:00:00

  • Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains.

    abstract::Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA. Glycosylation of the enzymes does not significantly affect their kinetic properties, or their stabilities towards heat and pH. However, the glycosylated enzymes ar...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)81150-x

    authors: Langsford ML,Gilkes NR,Singh B,Moser B,Miller RC Jr,Warren RA,Kilburn DG

    更新日期:1987-12-10 00:00:00

  • A relationship between the starting secondary structure of recombinant porcine growth hormone solubilised from inclusion bodies and the yield of native (monomeric) protein after in vitro refolding.

    abstract::Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80661-y

    authors: Puri NK,Cardamone M

    更新日期:1992-07-06 00:00:00

  • Mutational analysis of 6-aminohexanoate-dimer hydrolase: relationship between nylon oligomer hydrolytic and esterolytic activities.

    abstract::Carboxylesterase (EII') from Arthrobacter sp. KI72 has 88% homology to 6-aminohexanoate-dimer hydrolase (EII) and possesses ca. 0.5% of the level of 6-aminohexanoate-linear dimer (Ald)-hydrolytic activity of EII. To study relationship between Ald-hydrolytic and esterolytic activities, random mutations were introduced ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.08.031

    authors: Ohki T,Wakitani Y,Takeo M,Yasuhira K,Shibata N,Higuchi Y,Negoro S

    更新日期:2006-09-18 00:00:00

  • Sialic acid is an essential moiety of mucin as a hydroxyl radical scavenger.

    abstract::In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.04.062

    authors: Ogasawara Y,Namai T,Yoshino F,Lee MC,Ishii K

    更新日期:2007-05-29 00:00:00

  • A TY1 element is inserted in the CYR1 control region of Saccharomyces cerevisiae strain AB320.

    abstract::Southern blotting using a 5'-proximal probe of the Saccharomyces cerevisiae CYR1 gene has revealed heterogeneity in laboratory strains. It is demonstrated that strain AB320 contains a Ty1 element inserted in the promoter region of CYR1. The Ty1 orientation suggests that transcription of CYR1 is initiated downstream fr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)81227-9

    authors: Lenzen G,Masson P,Jacquemin JM,Danchin A

    更新日期:1987-07-13 00:00:00

  • Crystal structure of cce_0566 from Cyanothece 51142, a protein associated with nitrogen fixation in the DUF269 family.

    abstract::The crystal structure for cce_0566 (171 aa, 19.4 kDa), a DUF269 annotated protein from the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142, was determined to 1.60Å resolution. Cce_0566 is a homodimer with each molecule composed of eight α-helices folded on one side of a three strand anti-parallel β-sheet. Hydrop...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2012.01.037

    authors: Buchko GW,Robinson H

    更新日期:2012-02-17 00:00:00

  • Na(+)-driven ATP synthesis in Methanobacterium thermoautotrophicum and its differentiation from H(+)-driven ATP synthesis by rhodamine 6G.

    abstract::Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00535-4

    authors: Smigán P,Majerník A,Greksák M

    更新日期:1994-06-27 00:00:00

  • 9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene.

    abstract::The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)00830-2

    authors: Poirier H,Braissant O,Niot I,Wahli W,Besnard P

    更新日期:1997-08-04 00:00:00

  • The effect of glycosyl inositol-phosphate on cAMP production in isolated rat fat-cells is transduced by a pertussis toxin sensitive G-protein.

    abstract::In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80951-x

    authors: Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

    更新日期:1991-07-29 00:00:00

  • The C-terminal domain of yeast Ero1p mediates membrane localization and is essential for function.

    abstract::In eukaryotes, members of the Ero1 family control oxidative protein folding in the endoplasmic reticulum (ER). Yeast Ero1p is tightly associated with the ER membrane, despite cleavage of the leader peptide, the only hydrophobic sequence that could mediate lipid insertion. In contrast, human Ero1-Lalpha and a yeast mut...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03034-4

    authors: Pagani M,Pilati S,Bertoli G,Valsasina B,Sitia R

    更新日期:2001-11-09 00:00:00

  • MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development.

    abstract::Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.08.061

    authors: Santelia D,Vincenzetti V,Azzarello E,Bovet L,Fukao Y,Düchtig P,Mancuso S,Martinoia E,Geisler M

    更新日期:2005-10-10 00:00:00

  • Sirtuin-mediated deacetylation pathway stabilizes Werner syndrome protein.

    abstract::Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.06.031

    authors: Kahyo T,Mostoslavsky R,Goto M,Setou M

    更新日期:2008-07-23 00:00:00

  • Modulation by (iso)flavonoids of the ATPase activity of the multidrug resistance protein.

    abstract::The multidrug resistance protein (MRP) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. In this study we report that dinitrophenyl-S-glutathione increases ATPase activity in plasma membrane vesicles prepared from the MRP-overexpressing cell line GLC4...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)00940-x

    authors: Hooijberg JH,Broxterman HJ,Heijn M,Fles DL,Lankelma J,Pinedo HM

    更新日期:1997-08-18 00:00:00

  • Immunological detection of arrestin, a phototransduction regulatory protein, in the cytosol of nucleated erythrocytes.

    abstract::Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extract...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81663-1

    authors: Mirshahi M,Borgese F,Razaghi A,Scheuring U,Garcia-Romeu F,Faure JP,Motais R

    更新日期:1989-12-04 00:00:00

  • Characterization of early induced genes in Arabidopsis thaliana responding to bacterial inoculation: identification of centrin and of a novel protein with two regions related to kinase domains.

    abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01015-1

    authors: Cordeiro MC,Piqueras R,de Oliveira DE,Castresana C

    更新日期:1998-09-04 00:00:00

  • Evolution of tuf genes: ancient duplication, differential loss and gene conversion.

    abstract::The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02639-4

    authors: Lathe WC 3rd,Bork P

    更新日期:2001-08-03 00:00:00

  • Mapping the heparin-binding domain of boar spermadhesins.

    abstract::Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column dig...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01513-2

    authors: Calvete JJ,Dostàlova Z,Sanz L,Adermann K,Thole HH,Töpfer-Petersen E

    更新日期:1996-02-05 00:00:00

  • TGF-β triggers HBV cccDNA degradation through AID-dependent deamination.

    abstract::The covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is a viral center molecule for HBV infection and persistence. However, the cellular restriction factors of HBV cccDNA are not well understood. Here, we show that TGF-β can induce nuclear viral cccDNA degradation and hypermutation via activation-ind...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.12058

    authors: Qiao Y,Han X,Guan G,Wu N,Sun J,Pak V,Liang G

    更新日期:2016-02-01 00:00:00

  • Human macrophage inflammatory protein-3alpha/CCL20/LARC/Exodus/SCYA20 is transcriptionally upregulated by tumor necrosis factor-alpha via a non-standard NF-kappaB site.

    abstract::The 5'-flanking sequences of the human macrophage inflammatory protein-3alpha/CCL20 gene were cloned and transfected into G-361 human melanoma cells in a luciferase reporter construct. Tumor necrosis factor-alpha (TNF-alpha) treatment stimulated luciferase expression, and promoter truncations demonstrated that TNF-alp...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03138-6

    authors: Harant H,Eldershaw SA,Lindley IJ

    更新日期:2001-12-14 00:00:00

  • Characterization of type II thioesterases involved in natamycin biosynthesis in Streptomyces chattanoogensis L10.

    abstract::The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2014.07.010

    authors: Wang YY,Ran XX,Chen WB,Liu SP,Zhang XS,Guo YY,Jiang XH,Jiang H,Li YQ

    更新日期:2014-08-25 00:00:00

  • The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis.

    abstract::Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.02.060

    authors: Mueller M,Nidetzky B

    更新日期:2007-04-03 00:00:00

  • Interdomain interaction through helices A and B of DnaK peptide binding domain.

    abstract::In order to better define the structural elements involved in allosteric signalling, wild-type DnaK and three deletion mutants of the peptide binding domain have been characterized by biophysical (steady-state and time-resolved fluorescence) and biochemical methods. In the presence of ATP the chemical environment of t...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03752-3

    authors: Moro F,Fernández V,Muga A

    更新日期:2003-01-02 00:00:00

  • Purification and characterization of phospholipase A2 inhibitory proteins from pig thyroid gland.

    abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)81273-0

    authors: Antonicelli F,Rothhut B,Martiny L,Aguie-Aguie G,Lambert B,Bellon G,Russo-Marie F,Jacquemin C,Haye B

    更新日期:1988-08-01 00:00:00

  • Structure of membrane-bound human factor Va.

    abstract::Coagulation factor Va is an essential cofactor which combines with the serine protease factor Xa on a phospholipid surface to form the prothrombinase complex. In the present study, the structure of factor Va interacting with lipid surfaces containing phosphatidylserine was studied by electron microscopy. Two-dimension...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00881-7

    authors: Stoylova S,Mann KG,Brisson A

    更新日期:1994-09-12 00:00:00