Abstract:
:Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehyde C1 demonstrates a specific protonation of the corresponding intermediate by the enzyme. Proton inventory studies and enzyme modification indicate the 4' amino group of the coenzyme, thiamine pyrophosphate, in an immonium structure being a possible proton donor. A 'partially concerted' mechanism is suggested for the reaction steps following the decarboxylation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ermer J,Schellenberger A,Hübner Gdoi
10.1016/0014-5793(92)80238-ckeywords:
subject
Has Abstractpub_date
1992-03-09 00:00:00pages
163-5issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(92)80238-Cjournal_volume
299pub_type
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