Abstract:
:A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA templates was performed using water-soluble carbodiimide, phosphodiester and pyrophosphate internucleotide bonds being newly formed. Such chemical ligation proved to be a sensitive monitor of changes in the sugar-phosphate backbone resulting from conversion of double to triple helix and of third-strand binding.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dolinnaya NG,Pyatrauskene OV,Shabarova ZAdoi
10.1016/0014-5793(91)80692-vsubject
Has Abstractpub_date
1991-06-24 00:00:00pages
232-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80692-Vjournal_volume
284pub_type
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