Time-resolved fluorescence studies on site-directed mutants of human serum albumin.

abstract：:The retinal pigment epithelium-specific 65 kDa (RPE65) isomerase plays a pivotal role in photoreceptor survival and function. RPE65-catalyzed synthesis of 11-cis-retinol from all-trans-retinyl esters in the visual cycle is negatively regulated, through a heretofore unknown mechanism, by the fatty acid transport protei...

journal_title：FEBS letters

authors： Li S,Green JF,Jin M

更新日期：2020-02-01 00:00:00

abstract：:Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...

journal_title：FEBS letters

authors： Gaul G,Dutly F,Frei K,Foguet M,Lübbert H

更新日期：1992-08-03 00:00:00

abstract：:The post-translational modification of histone proteins plays an important role in controlling cell fate by directing essentially all DNA-associated nuclear processes. Misregulation and mutation of histone modifying enzymes is a hallmark of tumorigenesis. However, how these different epigenetic modifications lead to t...

journal_title：FEBS letters

authors： Johnsen SA

更新日期：2012-06-04 00:00:00

abstract：:In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-ter...

journal_title：FEBS letters

authors： Scarselli E,Esposito G,Traboni C

更新日期：1993-08-23 00:00:00

abstract：:The bacterial toxin VacA produced by H. pylori induces large vacuoles in several types of cultured cells such as HeLa cells or gastric cells. To determine the mechanism of vacuolation induced by this toxin we employed several inhibitors of membrane trafficking and endocytosis. The development of vacuolation induced by...

journal_title：FEBS letters

authors： Seto K,Hayashi-Kuwabara Y,Yoneta T,Suda H,Tamaki H

更新日期：1998-07-24 00:00:00

abstract：:RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense RNA, and consequently they are refractory to transformation by various oncogenes. In RCR cells, MAP kinases (ERK1 and ERK2) were activated to an extent and in a time course similar to those of the original NRK cells, irresp...

journal_title：FEBS letters

authors： Kizaka-Kondoh S,Okayama H

更新日期：1993-12-27 00:00:00

abstract：:Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digitonin-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 microM, while ...

journal_title：FEBS letters

authors： Eichholtz T,Alblas J,van Overveld M,Moolenaar W,Ploegh H

更新日期：1990-02-12 00:00:00

abstract：:Nuclear cap-binding protein (CBP) 80/20-dependent translation (CT) is one of the targets for miRNA-mediated gene silencing. Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is tightly coupled to CT. Teth...

journal_title：FEBS letters

authors： Choe J,Cho H,Chi SG,Kim YK

更新日期：2011-09-02 00:00:00

abstract：:Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...

journal_title：FEBS letters

authors： Xu X,Leng JY,Gao F,Zhao ZA,Deng WB,Liang XH,Zhang YJ,Zhang ZR,Li M,Sha AG,Yang ZM

更新日期：2014-05-02 00:00:00

abstract：:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...

journal_title：FEBS letters

authors： Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SM

更新日期：2006-04-03 00:00:00

abstract：:The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplac...

journal_title：FEBS letters

authors： Kovacevic A,Hammer A,Sundl M,Pfister B,Hrzenjak A,Ray A,Ray BK,Sattler W,Malle E

更新日期：2006-01-09 00:00:00

abstract：:A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...

journal_title：FEBS letters

authors： Mitchell DM,Gennis RB

更新日期：1995-07-10 00:00:00

abstract：:Telomerase is an almost universal cancer target that consists minimally of a core protein human telomerase reverse transcriptase (hTERT) and a RNA component human telomerase RNA (hTR). Some inhibitors of this enzyme are thought to function by the covalent binding to one or several cysteine residues; however, this inhi...

journal_title：FEBS letters

authors： Kellermann G,Dingli F,Masson V,Dauzonne D,Ségal-Bendirdjian E,Teulade-Fichou MP,Loew D,Bombard S

更新日期：2017-03-01 00:00:00

abstract：:The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...

journal_title：FEBS letters

authors： Reindl A,Schöffl F

更新日期：1998-10-09 00:00:00

abstract：:We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...

journal_title：FEBS letters

authors： Maeda K,Rösch A,Maéda Y,Kalbitzer HR,Wittinghofer A

更新日期：1991-04-09 00:00:00

abstract：:Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...

journal_title：FEBS letters

authors： Labhart P

更新日期：1994-12-19 00:00:00

abstract：:14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...

journal_title：FEBS letters

authors： Yaffe MB

更新日期：2002-02-20 00:00:00

abstract：:L-selectin guides lymphocytes into peripheral lymphoid tissues by recognizing glycoprotein ligands decorated with 6-sulfated sialyl Lewis x (sulfo sLex). Here we have used a rat peripheral lymph node high endothelial cell line (Ax) to study in detail the synthesis, expression and degradation of sLex epitope. We show h...

journal_title：FEBS letters

authors： Majuri ML,Räbinä J,Niittymäki J,Tiisala S,Mattila P,Aavik E,Miyasaka M,Renkonen O,Renkonen R

更新日期：1999-07-16 00:00:00

abstract：:The enantiomers of U50,488, ligands highly selective for kappa-opioid receptors, have been prepared by a refined procedure and their optical purity demonstrated. The absolute configuration of (+)-trans-2-pyrrolidinyl-N-methylcyclohexylamine, a chemically versatile intermediate for synthesis of analogs of kappa-opioid ...

journal_title：FEBS letters

authors： DeCosta B,George C,Rothman RB,Jacobson AE,Rice KC

更新日期：1987-11-02 00:00:00

abstract：:We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin alpha, somatostatin, immunoglobulin kappa chain, alpha crystallin, albumin and interferon-beta promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovir...

journal_title：FEBS letters

authors： Toyama R,Okayama H

更新日期：1990-07-30 00:00:00

abstract：:Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The 'dissected' molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two f...

journal_title：FEBS letters

authors： Boutorin AS,Vassilenko SK,Baklanov MM,Nechaev YS

更新日期：1984-01-02 00:00:00

abstract：:Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

journal_title：FEBS letters

authors： Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

更新日期：2017-09-01 00:00:00

abstract：:Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ ...

journal_title：FEBS letters

authors： Webster GT,Tilley L,Deed S,McNaughton D,Wood BR

更新日期：2008-04-02 00:00:00

abstract：:Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecip...

journal_title：FEBS letters

authors： Peng X,Zhang Y,Zhang H,Graner S,Williams JF,Levitt ML,Lokshin A

更新日期：1999-03-05 00:00:00

abstract：:Monomeric bovine pancreatic RNase A has been transformed into a dimeric ribonuclease with antitumor activity (Di Donato, A., Cafaro, V. and D'Alessio, G. (1994) J. Biol. Chem. 269, 17394-17396). This was accomplished by replacing the residues located in the RNase chain at positions 19, 28, 31, and 32, with proline, le...

journal_title：FEBS letters

authors： Cafaro V,Bracale A,Di Maro A,Sorrentino S,D'Alessio G,Di Donato A

更新日期：1998-10-16 00:00:00

abstract：:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

journal_title：FEBS letters

authors： Pardridge WM,Boado RJ

更新日期：1991-08-19 00:00:00

abstract：:Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771...

journal_title：FEBS letters

authors： Pei ZD,Williamson JR

更新日期：1998-02-13 00:00:00

abstract：:When bottromycin A2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl-tRNA to the polyribosomes was inhibited by bottromycin A2. In con...

journal_title：FEBS letters

authors： Otaka T,Kaji A

更新日期：1983-03-07 00:00:00

abstract：:ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated met...

journal_title：FEBS letters

authors： Adolph KW,Song MK

更新日期：1985-03-11 00:00:00

abstract：:Parasitic helminths remain major pathogens of both humans and animals throughout the world. The success of helminth infections depends on the capacity of the parasite to counteract host immune responses but also to exploit host-derived signal molecules for its development. Recent progress has been made in the characte...

journal_title：FEBS letters

authors： Dissous C,Khayath N,Vicogne J,Capron M

更新日期：2006-05-22 00:00:00