Abstract:
:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate GPR motifs. The WAVE1 GPR peptide inhibited GTPgammaS binding to purified G-protein. Endogenous Gialpha and WAVE1 coimmunoprecipitated from brain lysates. A WAVE1-G-protein complex was also observed following transfection of COS7 cells with Gialpha3 and WAVE1. The docking of Gialpha within a WAVE1 scaffolding complex may facilitate dynamic cycling and/or targeting for efficient and localized control of actin polymerization.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SMdoi
10.1016/j.febslet.2006.02.071keywords:
subject
Has Abstractpub_date
2006-04-03 00:00:00pages
1993-8issue
8eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00274-2journal_volume
580pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Multidrug transporters are involved in mediating the failure of chemotherapy in treating several serious diseases. The archetypal multidrug transporter P-glycoprotein (P-gp) confers resistance to a large number of chemically and functionally unrelated anti-cancer drugs by mediating efflux from cancer cells. The abilit...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.11.083
更新日期:2006-02-13 00:00:00
abstract::The formation of coenzyme A thiol esters of cinnamic, p-coumaric, p-methoxy cinnamic, and ferulic acids was catalyzed by enzyme preparations from cell suspension cultures of leaf petioles from parsley (Petroselinum hortense Hoffm.). Of these acids, p-coumaric acid served as the most efficient substrate. Enzyme activit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80492-6
更新日期:1970-11-09 00:00:00
abstract::A cDNA encoding stromal ascorbate peroxidase (sAPX) was isolated using poly(A)+ RNA of pumpkin cotyledons by RT-PCR. The cDNA encodes a polypeptide with 372 amino acids and shares complete sequence identity with pumpkin thylakoid-bound ascorbate peroxidase (tAPX), except for the deletion of a putative membrane spannin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00862-4
更新日期:1997-08-11 00:00:00
abstract::Electron microscopy of mammalian smooth muscle myosin rods showed them to be 153 +/- 7 nm (SD) long, and to bend sharply (greater than 90 degrees) but infrequently, and pH independently (range 6.5-9.5), at a single site 45 +/- 4 nm from one end of the molecule. Light meromyosin (LMM) preparations were 99 +/- 10 nm lon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80940-0
更新日期:1984-10-15 00:00:00
abstract::Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12716
更新日期:2017-08-01 00:00:00
abstract::Mutations in the nuclear matrix protein Matrin 3 (MATR3) have been identified in amyotrophic lateral sclerosis and myopathy. To investigate the mechanisms underlying MATR3 mutations in neuromuscular diseases and efficiently screen for modifiers of MATR3 toxicity, we generated transgenic MATR3 flies. Our findings indic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13858
更新日期:2020-09-01 00:00:00
abstract::The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80236-2
更新日期:1985-01-21 00:00:00
abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01365-8
更新日期:1995-01-03 00:00:00
abstract::It is 40 years since cancer growth was correlated with neovascularisation. Anti-angiogenic drugs remain at the forefront of cancer investigations but progress has been disappointing and unexpected toxicities are emerging. Gap junction channels are implicated in lesion spread following injury, with channel blockers sho...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.02.003
更新日期:2014-04-17 00:00:00
abstract::The metabolism of the double bonds at the delta 3 position in fatty acids was studied in rat liver. Infusion of delta 3-trans-dodecenoic acid into isolated perfused liver and subcellular fractionation studies showed the presence of both peroxisomal and mitochondrial delta 3,delta 2-enoyl-CoA isomerase activity (EC 5.3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80151-5
更新日期:1987-05-11 00:00:00
abstract::Raman spectra of active Na+,K+-ATPase from pig kidney and membrane-bound products of its two-stage trypsinolysis, including alpha-subunit hydrophobic regions as well as the intact beta-subunit and hydrophobic regions of alpha- and beta-subunits, were measured to calculate the secondary structure of hydrophilic and hyd...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80905-0
更新日期:1988-01-25 00:00:00
abstract::Polysulfide is a bound sulfur species derived from endogenous H2S. When mouse neuroblastoma, Neuro2A cells were exposed to tert-butyl hydroperoxide after treatment with polysulfide, a significant decline in cell toxicity was observed. Rapid uptake of polysulfides induced translocation of Nrf2 into the nucleus, resulti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.013
更新日期:2013-11-01 00:00:00
abstract::The hypothesis that calpain subunits dissociate in the presence of Ca2+ has been tested by methods which avoid interference by Ca2+-induced aggregation and large subunit autolysis. Inactive Cys105Ser-m-calpain, bound either to Ni-NTA-agarose or to immobilized casein, after incubation with Ca2+, could be recovered in h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01167-3
更新日期:1998-10-09 00:00:00
abstract::In the bovine ovary there is a delay of 4-6 days between the observed maximum of oxytocin mRNA and the peak in the luteal levels of oxytocin nonapeptide. This implies a maturation process involving components of the post-translational processing pathway. In situ hybridization shows the oxytocin gene to be transcribed ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81295-4
更新日期:1987-01-01 00:00:00
abstract::The photochemical reaction center of Chloroflexus (Cf.) aurantiacus, a membrane bound pigment-protein complex, has been crystallized in the presence of monodisperse polyoxyethylene detergents. The crystals possessed a pronounced polymorphism. Three different crystal forms belonging to triclinic, monoclinic and orthorh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01101-5
更新日期:1996-11-04 00:00:00
abstract::The 36 kDa fragment of actin molecule obtained with the protease from E. coli A2 strain [(1988) FEBS Lett. 228, 172] was shown to begin with Val-43 and retain the COOH-terminal amino acid residues of the parent molecule. The E. coli protease split actin preserves the NH2-terminal part of the polypeptide chain as well ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80247-z
更新日期:1991-02-11 00:00:00
abstract::Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12780
更新日期:2017-09-01 00:00:00
abstract::The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutans, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81632-1
更新日期:1989-11-20 00:00:00
abstract::Although transthyretin (TTR) is expressed in pancreatic alpha (glucagon) cells in the islets of Langerhans, the function of TTR in pancreatic alpha cells remains unknown. In this study, by using TTR knockout (TTR KO) mice, we determined the novel role of TTR in glucose homeostasis. We demonstrated that TTR KO mice evi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.10.025
更新日期:2012-11-30 00:00:00
abstract::F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.030
更新日期:2005-09-26 00:00:00
abstract::The effect of neuropeptide Y (NPY) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured smooth muscle cells from porcine aorta (PASMC) and compared with the effect of bradykinin (BK) and angiotensin II (ATII) on [Ca2+]i. All peptides induced dose-dependent and transient rises in [Ca2+]i which were no...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81499-1
更新日期:1989-12-18 00:00:00
abstract::Protective antigen (PA) is the central receptor binding component of anthrax toxin, which translocates catalytic components of the toxin into the cytosol of mammalian cells. Ever since the crystal structure of PA was solved, there have been speculations regarding the possible role of calcium ions present in domain I o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01226-2
更新日期:2003-11-20 00:00:00
abstract::Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81526-0
更新日期:1987-03-23 00:00:00
abstract::Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80120-x
更新日期:1986-01-20 00:00:00
abstract::In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins of the cell-cell junctions. We report the direct interaction between hScrib and ZO-2, a junction-associated protein. This inter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.062
更新日期:2005-07-04 00:00:00
abstract::Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81660-r
更新日期:1993-03-29 00:00:00
abstract::The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80452-6
更新日期:1988-06-20 00:00:00
abstract::Glycinebetaine (betaine), an osmoregulant in halophilic plants, stabilized the evolution of oxygen and the synthesis of ATP by thylakoid membranes from the cyanobacterium Synechocystis PCC6803 when it was present during the preparation and incubation of the thylakoid membranes. Moreover, betaine enhanced the evolution...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81446-f
更新日期:1991-12-09 00:00:00
abstract::Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.050
更新日期:2010-05-03 00:00:00
abstract::Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00163-4
更新日期:1997-03-17 00:00:00