Abstract:
:The effect of neuropeptide Y (NPY) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured smooth muscle cells from porcine aorta (PASMC) and compared with the effect of bradykinin (BK) and angiotensin II (ATII) on [Ca2+]i. All peptides induced dose-dependent and transient rises in [Ca2+]i which were not blocked by extracellular EGTA, but the NPY response was different from the others' as follows. First, the [Ca2+]i rise induced by NPY was not as rapid as that induced by BK or ATII. Second, pertussis toxin abolished the [Ca2+]i rise induced by NPY, but not by BK or ATII. Third, following initial treatment with BK, PASMC were able to respond to NPY, but not to ATII. Finally, BK and ATII, but not NPY, significantly increased inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) generation. Although NPY attenuated forskolin-induced accumulation of cyclic AMP, forskolin- and 3-isobutyl-1-methyl-xanthine-induced alterations in intracellular cyclic AMP did not affect the NPY-induced [Ca2+]i rise. These results suggest that NPY increases [Ca2+]i by a pertussis toxin-sensitive GTP binding protein-involved mechanism which is not mediated by the intracellular messengers such as Ins(1,4,5)P3 and cyclic AMP.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mihara S,Shigeri Y,Fujimoto Mdoi
10.1016/0014-5793(89)81499-1subject
Has Abstractpub_date
1989-12-18 00:00:00pages
79-82issue
1eissn
0014-5793issn
1873-3468journal_volume
259pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Physiological and pathophysiological differences exist between the left and right kidneys; however, the molecular bases for these differences remain unknown. Since miRNAs are involved in kidney function and the development of kidney diseases, we examined their differential expression through miRNA profiling of the lef...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13644
更新日期:2020-02-01 00:00:00
abstract::The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80184-2
更新日期:1984-01-09 00:00:00
abstract::Under conditions of molar excess of enzyme, isolated F1-ATPase from beef heart mitochondria catalyses ATP hydrolysis biphasically. The rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. The slow phase of ATP hydrolysis is insensitive to EDTA. [gamma-32P]ATP splitting in th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80186-2
更新日期:1987-09-28 00:00:00
abstract::The functionality of the disintegrin-like/cysteine-rich domains of snake venom metalloproteinases (SVMPs) has been shown to reside in the cysteine-rich region, which can interact with VWA-containing proteins. Recently, the hyper-variable region (HVR) of the cysteine-rich domain was suggested to constitute a potential ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.10.034
更新日期:2008-11-26 00:00:00
abstract::We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.042
更新日期:2006-05-29 00:00:00
abstract::The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01144-3
更新日期:2000-02-11 00:00:00
abstract::Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01668-8
更新日期:1999-01-15 00:00:00
abstract::Human Tid-1 (hTid-1) is a DnaJ chaperone protein with homology to the Drosophila tumor suppressor Tid56. We report the first case of a tumor-associated mutation at the human TID1 locus, which was identified in the SF767 glioma cell line giving rise to aberrantly high levels of a hTid-1(L) mutant variant. In this study...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.034
更新日期:2004-12-17 00:00:00
abstract::There are two secondary structure models for the eukaryotic selenocysteine (Sec) tRNA(Sec). One model, the 9/4 structure, was experimentally tested and possesses acceptor and T-stems with 9 and 4 bp, respectively [Sturchler et al., 1993; Hubert et al., 1998]. The other one, the 7/5 secondary structure with a bulge in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01104-2
更新日期:2000-01-28 00:00:00
abstract::Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80157-x
更新日期:1991-02-25 00:00:00
abstract::The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.10.043
更新日期:2007-11-27 00:00:00
abstract::Accumulating evidence has indicated that microRNAs (miRNAs) act as critical epigenetic regulators in tumor carcinogenesis. Here, we report that miR-199a-3p was significantly upregulated in gastric cancer (GC) cell lines and tissues. Functional studies demonstrated that miR-199a-3p dramatically increased cell prolifera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.09.047
更新日期:2014-11-28 00:00:00
abstract::Cell-substratum adhesion of rat hepatocytes was inhibited by antisera raised against plasma membranes of liver (anti-liver antiserum) and Morris hepatoma 7777 (anti-hepatoma antiserum). Similar concentrations of both antisera inhibited adhesion on collagen. Anti-liver antiserum also inhibited the adhesion of hepatocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80254-9
更新日期:1984-03-26 00:00:00
abstract::In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01298-2
更新日期:1997-11-17 00:00:00
abstract::Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00945-6
更新日期:1995-09-18 00:00:00
abstract::We describe the extraction and stabilization in vitro of discrete complexes of interferon and cellular receptor proteins. A homogeneous complex of Mr 230 000 was extracted at the time of peak receptor binding (30 min). Complex formation was specific for human interferon. At later times a second complex could also be e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80268-3
更新日期:1983-05-30 00:00:00
abstract::Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81299-2
更新日期:1992-08-24 00:00:00
abstract::We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01407-r
更新日期:1995-01-23 00:00:00
abstract::The catalytic cycle of heme peroxidases involves two reactive states, compound I and compound II. Although their reduction potentials at pH 7 are similar, compound I is in general more reactive towards organic substrates than compound II. The different reactivities have until now remained unexplained. In this study, t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00792-8
更新日期:1997-07-28 00:00:00
abstract::Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblas...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12561
更新日期:2017-02-01 00:00:00
abstract::Molecular dynamics simulations have been performed on the intrinsically disordered 39-residue N-terminal transactivation domain of p53 (p53(1-39)). Simulations not only revealed that p53(1-39) is natively compact, but also possesses a folded structure. Furthermore, leucine-rich hydrophobic clusters were found to play ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.060
更新日期:2009-02-04 00:00:00
abstract::The F-box protein Skp2 is important for S phase entry and binds to Skp1 and the cyclin A-Cdk2 complex. Here we report the cloning, analysis of genomic organization and characterization of a novel gene product related to Skp2 named FBL2. The human FBL2 gene was found to be a highly interrupted gene of at least 126.6 kb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01211-9
更新日期:1999-10-01 00:00:00
abstract::Renin, which catalyzes the initial proteolytic cleavage reaction in the production of angiotensins, is first synthesized as a zymogen, prorenin, and requires the proteolytic removal of an amino-terminal prosegment for activation in vivo. The lysosomal hydrolase cathepsin B has been proposed as a prorenin processing en...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01672-x
更新日期:1999-01-22 00:00:00
abstract::Light-dependent Na+ and H+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium Oscillatoria brevis. In the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular Na+ level is shown to increase in the dark and d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81268-s
更新日期:1990-09-17 00:00:00
abstract::Ample evidence indicates that sphingosine-1-phosphate (SPP) can serve as an intracellular second messenger regulating calcium mobilization, and cell growth and survival. Moreover, the dynamic balance between levels of the sphingolipid metabolites, ceramide and SPP, and consequent regulation of opposing signaling pathw...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)01670-7
更新日期:2000-06-30 00:00:00
abstract::A distinctive tract of all the forms of Alzheimer's disease is the extracellular deposition of a 40-42/43 amino acid-long peptide derived from the so-called beta-amyloid precursor protein (APP). This is a membrane protein of unknown function, whose short cytosolic domain has been recently demonstrated to interact with...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(98)00941-7
更新日期:1998-08-28 00:00:00
abstract::Nerve growth factor (NGF), basic fibroblast growth factor (bFGF), dibutyryl cAMP and forskolin, known differentiating agents for pheochromocytoma PC12 cells, induced sustained activation of sphingosine kinase, the enzyme responsible for the formation of the sphingolipid second messenger, sphingosine-1-phosphate, which...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01277-5
更新日期:1997-11-10 00:00:00
abstract::It is 40 years since cancer growth was correlated with neovascularisation. Anti-angiogenic drugs remain at the forefront of cancer investigations but progress has been disappointing and unexpected toxicities are emerging. Gap junction channels are implicated in lesion spread following injury, with channel blockers sho...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.02.003
更新日期:2014-04-17 00:00:00
abstract::Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.035
更新日期:2010-11-19 00:00:00
abstract::Erythroid spectrin is the main component of the red cell membrane skeleton, which is very important in determining the shape, resistance to mechanical stresses and deformability of red cells. Previously we demonstrated that human erythroid alpha-spectrin is ubiquitinated in vitro and in vivo, and using recombinant pep...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02333-4
更新日期:2001-02-02 00:00:00