Abstract:
:Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell line dependent on either GM-CSF or interleukin-3 (IL-3). The inhibitory effect of PAP-BALF occurred only when TF-1 cells were cultured with GM-CSF but not when cultured with IL-3, suggesting that PAP-BALF contains a factor that specifically interferes with GM-CSF function. 125I-GM-CSF binding to TF-1 cells was prevented in the presence of BALF from IPAP patients. Furthermore, cross-linking of 125I-GM-CSF to IPAP-BALF produced two major bands on SDS-PAGE; these bands were not observed in normal BALF. These data suggest that IPAP is caused by expression of binding factor(s) which inhibit GM-CSF function in the lung.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tanaka N,Watanabe J,Kitamura T,Yamada Y,Kanegasaki S,Nakata Kdoi
10.1016/s0014-5793(98)01668-8subject
Has Abstractpub_date
1999-01-15 00:00:00pages
246-50issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)01668-8journal_volume
442pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.11.036
更新日期:2015-12-21 00:00:00
abstract::The HBx protein of hepatitis B virus is involved in deregulation of cell cycle and development of hepatocellular carcinoma. Since c-Myc also plays an important role in cell proliferation and tumor development, we studied its regulation by HBx in a human hepatoma cell line. Co-expression of HBx and c-Myc resulted in in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.034
更新日期:2006-01-23 00:00:00
abstract::Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01121-2
更新日期:2000-02-04 00:00:00
abstract::FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02915-5
更新日期:2001-10-19 00:00:00
abstract::As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear fa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.048
更新日期:2013-10-01 00:00:00
abstract::The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative su...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.10.011
更新日期:2015-11-30 00:00:00
abstract::cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81453-x
更新日期:1989-07-17 00:00:00
abstract::A truncated human Na(+)/glucose cotransporter (C(5), residues 407-664) was expressed and purified from Escherichia coli using a GST fusion vector and glutathione affinity chromatography. The truncated transporter (C(5)) was cleaved from GST-C(5) by Factor Xa proteolysis and purified by gel filtration chromatography. U...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01292-2
更新日期:1999-10-15 00:00:00
abstract::In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03010-1
更新日期:2001-11-09 00:00:00
abstract::We have analyzed the glycosaminoglycans exposed on the surface of endothelial cells cultured in vitro. The cells were labelled with [35S]sodium sulfate. Chondroitin sulfate, heparan sulfate and dermatan sulfate are the main sulfated glycosaminoglycans present on the cell surface. They are synthesized by endothelial ce...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81469-g
更新日期:1993-05-24 00:00:00
abstract::The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80437-x
更新日期:1988-06-20 00:00:00
abstract::The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both puri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80241-8
更新日期:1992-03-09 00:00:00
abstract::Maculatin 1.1 is an antimicrobial peptide isolated from the Australian tree frog Litoria genimaculata that adopts an amphipathic, alpha-helical structure in solution. Its orientation and conformation when incorporated to pre-formed DMPG (1,2-dimyristoyl-sn-glycero-3-phosphoglycerol) and DMPC (1,2-dimyristoyl-sn-glycer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03313-0
更新日期:2002-02-13 00:00:00
abstract::Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01196-x
更新日期:1998-10-16 00:00:00
abstract::JAK2 V617F mutant induces transformation through aberrant activation of various transcription factors. We found that the expression of Aurora kinase A (Aurka) was significantly induced by mutant JAK2 through c-Myc expression. Interestingly, mutant JAK2 enhanced resistance to cisplatin (CDDP)-induced DNA damage, and ef...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.04.068
更新日期:2011-06-23 00:00:00
abstract::In order to elucidate the molecular mechanisms responsible for the specific lipid-protein interactions, we have undertaken structural and idiotypic analyses of a monoclonal antibody, PS4A7, which binds specifically to phosphatidylserine (PS). Here we showed that one of the anti-idiotypic monoclonal antibodies raised a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80421-4
更新日期:1994-02-21 00:00:00
abstract::The ATP synthase capacity of rat heart myocytes can be measured in sonicates of cultured cardiomyocytes. In these cells, transitions in ATP synthase capacity occur on changing to the anoxic or uncoupled state (drop in ATP synthase capacity of over 40%) or on electrically stimulating the cells to contract (rise of 70%)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81725-9
更新日期:1989-10-09 00:00:00
abstract::The success of Cre-mediated conditional gene targeting in liver of mice has until now depended on the generation of Cre recombinase transgenic mice or on viral-mediated transduction. Here, we sought to establish the feasibility of using hydrodynamic gene delivery of Cre recombinase into liver, using a ROSA26 EGFP mous...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.094
更新日期:2006-08-07 00:00:00
abstract::On incubation of 7-day-old wheat seedlings in the presence of [3H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (greater than 95%) in the fraction of heavy mitochondrial DNA (H-mt DNA; rho = 1.716 gm/cm3). Upon long (48-72 h) incubation of cut-off seedlings in water, the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80033-d
更新日期:1992-02-24 00:00:00
abstract::The control of physiological processes requires the regulation and coordination of many different signals and is determined in part by the activation and repression of expression of specific target genes. RIP140 is a ligand dependent coregulator of many nuclear receptors that influence such diverse processes as muscle...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.11.017
更新日期:2008-01-09 00:00:00
abstract::L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Ca2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4 beta-phorbol 12-myristate 13-acetate) were studied. Currents through channels co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80980-u
更新日期:1992-07-20 00:00:00
abstract::Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) incr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00178-9
更新日期:2003-03-27 00:00:00
abstract::Mg2+ -dependent catechol-O-methyltransferases occur in animals as well as in bacteria, fungi and plants, often with a pronounced selectivity towards one of the substrate's hydroxyl groups. Here, we show that the bacterial MxSafC exhibits excellent regioselectivity for para as well as for meta methylation, depending on...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12530
更新日期:2017-01-01 00:00:00
abstract::The possible relationship between pyridoxal phosphate-dependent ornithine decarboxylase (ODC) activity and glucose-6-phosphate dehydrogenase (G6PD) activity has been studied in the osteoblasts of the growth-plate of metatarsals of rats fed a pyridoxine-deficient diet, which caused depressed G6PD levels. The G6PD activ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80579-8
更新日期:1986-05-26 00:00:00
abstract::Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00177-4
更新日期:1997-03-24 00:00:00
abstract::We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with singl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.040
更新日期:2007-04-17 00:00:00
abstract::The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80203-3
更新日期:1994-01-17 00:00:00
abstract::SP-40,40 is a two-chain serum protein which acts in vitro as a potent inhibitor of the assembly of the membrane attack complex of human complement. It contains 10 cysteine residues, the numbers and locations of which are conserved in several mammalian species. Evidence is presented that all the cysteine residues are i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80330-j
更新日期:1992-02-03 00:00:00
abstract::The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80139-l
更新日期:1993-06-21 00:00:00
abstract::A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00757-6
更新日期:1997-07-28 00:00:00