Abstract:
:The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but the following millisecond-transition to Meta-R(C) is blocked. Consistently, no transient proton release was detected. The photoconversion of Agp1-5Zs is apparently arrested in a Meta-R(A)-like intermediate, since the subsequent syn to anti rotation around the C(5)-C(6) bond is prevented by the lock. The Meta-R(A)-like photoproduct was characterized by its distinctive CD spectrum suggesting a reorientation of ring D.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Seibeck S,Borucki B,Otto H,Inomata K,Khawn H,Kinoshita H,Michael N,Lamparter T,Heyn MPdoi
10.1016/j.febslet.2007.10.043subject
Has Abstractpub_date
2007-11-27 00:00:00pages
5425-9issue
28eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01108-8journal_volume
581pub_type
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