Abstract:
:Protamines are polycationic proteins that are widely used for neutralisation of the anticoagulant action of heparin. However, several reports have shown adverse, mast cell-dependent reactions to protamine. The exact mechanism by which protamine causes these adverse effects is not clear. In the present study, the possibility that protamine may influence mast cell chymase function was investigated. Mast cell chymase is in vivo recovered in a macromolecular complex with heparin proteoglycan, and this interaction is essential for expression of optimal enzymatic activity. Protamine was shown to strongly reduce the activity of mast cell chymase by a mechanism that involved displacement of the chymase from heparin proteoglycan.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pejler Gdoi
10.1016/0014-5793(96)00239-6subject
Has Abstractpub_date
1996-04-01 00:00:00pages
170-4issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(96)00239-6journal_volume
383pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Monoclonal antibodies (mAbs) against cell surface antigens and receptors are instrumental in defining specific membrane markers. mAbs GF 26.7.3 and MF 25.1 against human neutrophils modulated the activation mechanism of superoxide anion production induced by formyl-peptide and PMA in all subject. However, treatment wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81385-0
更新日期:1986-08-11 00:00:00
abstract::Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81119-s
更新日期:1991-10-07 00:00:00
abstract::Electrospray/mass spectrometry (ES/MS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates cont...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00966-x
更新日期:1994-10-03 00:00:00
abstract::TATA-box binding factor TFIID is one of the key factors in transcriptional activation. Surprisingly, the yeast TFDII protein [(1989) Nature 341, 299-303; (1989) Cell 56, 1173-1181; (1989) Proc. Natl. Acad. Sci USA 86, 7785-7789] reveals only limited homology with other DNA-binding proteins. From computer-assisted sear...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(90)80558-z
更新日期:1990-02-26 00:00:00
abstract::A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00696-1
更新日期:1999-06-25 00:00:00
abstract::Reliably pinpointing which specific amino acid residues form the interface(s) between a protein and its binding partner(s) is critical for understanding the structural and physicochemical determinants of protein recognition and binding affinity, and has wide applications in modeling and validating protein interactions...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.10.003
更新日期:2015-11-30 00:00:00
abstract::We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.028
更新日期:2009-01-05 00:00:00
abstract::We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca(2+)/CaM kinase II) completely repre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.05.062
更新日期:2004-07-02 00:00:00
abstract::Coeliac disease is a chronic enteropathy caused by the ingestion of wheat gliadin and other cereal prolamines derived from rye and barley. In the present work, we investigated the mechanisms underlying altered barrier function properties exerted by gliadin-derived peptides in human Caco-2 intestinal epithelial cells. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.066
更新日期:2005-08-29 00:00:00
abstract::Viperin is a radical SAM enzyme that possesses antiviral properties against a broad range of enveloped viruses. Here, we describe the activity of human viperin with two molecules of the mevalonate pathway, geranyl pyrophosphate, and farnesyl pyrophosphate, involved in cholesterol biosynthesis. We postulate that the ra...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12941
更新日期:2018-01-01 00:00:00
abstract::Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.049
更新日期:2006-10-02 00:00:00
abstract::In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12105
更新日期:2016-03-01 00:00:00
abstract::An Mr 21 000 polypeptide, designated APPG, has been purified by reverse-phase, high-performance liquid chromatography (RP-HPLC), from acid extracts of porcine anterior pituitary glands. This acidic protein possesses an isoelectric point of 4.9. Amino acid analysis shows that it is not a glycoprotein and estimates it t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81055-7
更新日期:1982-10-18 00:00:00
abstract::Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00477-q
更新日期:1995-06-05 00:00:00
abstract::In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.063
更新日期:2004-05-21 00:00:00
abstract::Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81132-2
更新日期:1986-12-15 00:00:00
abstract::The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81010-x
更新日期:1988-12-19 00:00:00
abstract::Mouse spermatozoa stimulated with epidermal growth factor (EGF) or zona pellucida (ZP) experienced phosphatidylinositol 4,5-bisphosphate hydrolysis, diacylglycerol (DAG) generation and acrosomal exocytosis. The agonists showed additive effects but the action of EGF is likely to be mediated by a distinct receptor becau...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00114-o
更新日期:1995-03-06 00:00:00
abstract::The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg2+. Addition of Mg2+ yields three partially reversible phase transitions at 18, 27 and 32 degrees C, respectively...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80295-6
更新日期:1983-12-12 00:00:00
abstract::Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81151-2
更新日期:1989-02-13 00:00:00
abstract::Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extract...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81663-1
更新日期:1989-12-04 00:00:00
abstract::Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00636-9
更新日期:1994-07-25 00:00:00
abstract::Different hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation. This study was specifically conducted to identify the 130 kDa biliary vesicle-ass...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80199-5
更新日期:1993-08-23 00:00:00
abstract::Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01075-5
更新日期:2003-10-23 00:00:00
abstract::Pyrroloquinoline quinone (PQQ) has been claimed to be a component of glutamate decarboxylase from Escherichia coli on the basis of a frequently used procedure in which the protein is extracted with hexanol. We demonstrate that if pyridoxal phosphate (PLP) is not added during the preparation, the apoenzyme prepared fro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80097-m
更新日期:1991-01-14 00:00:00
abstract::Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N'-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cul...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01111-8
更新日期:1996-11-04 00:00:00
abstract::The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01013-3
更新日期:1999-08-20 00:00:00
abstract::F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12071
更新日期:2016-02-01 00:00:00
abstract::Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80599-1
更新日期:1983-05-08 00:00:00
abstract::The effect of the novel, naturally occurring nucleotide cyclic diguanylic acid (c-di-GMP) on the lymphoblastoid CD4+ Jurkat cell line was studied. When exposed to 50 microM c-di-GMP, Jurkat cells exhibited a markedly elevated expression of the CD4 receptor of up to 6.3-fold over controls. C-di-GMP also causes blockage...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00036-8
更新日期:1999-02-05 00:00:00