Abstract:
:Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N'-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cultures as verified by flow cytometry and by visualisation of DNA with acridine orange staining. CAPE-induced apoptosis was inhibited by z-VAD-fmk, an inhibitor of the interleukin-1beta-converting enzyme family of cysteine proteases. We suggest that the lipoxygenase pathway of arachidonic acid metabolism plays a role in regulating lymphocyte responses such as proliferation and apoptosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mirzoeva OK,Yaqoob P,Knox KA,Calder PCdoi
10.1016/0014-5793(96)01111-8subject
Has Abstractpub_date
1996-11-04 00:00:00pages
266-70issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(96)01111-8journal_volume
396pub_type
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