Abstract:
:In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channels, but not the cell swelling-induced release of taurine, was largely inhibited in the presence of low concentrations of 1-butanol. In addition, hypotonicity-induced exocytosis, ATP release and subsequent endocytosis were found to be largely abrogated. The results support a model of cell volume regulation in which PLD plays an essential role in the cell swelling-induced vesicle cycling and in the activation of volume-sensitive anion channels.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tomassen SF,van der Wijk T,de Jonge HR,Tilly BCdoi
10.1016/j.febslet.2004.04.063keywords:
subject
Has Abstractpub_date
2004-05-21 00:00:00pages
287-90issue
1-3eissn
0014-5793issn
1873-3468pii
S001457930400540Xjournal_volume
566pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13588
更新日期:2020-01-01 00:00:00
abstract::Nanoscale protein supported phospholipid bilayer discs, or Nanodiscs, were produced for the purpose of studying the phase transition behavior of the incorporated lipids. Nanodiscs and vesicles were prepared with two phospholipids, dipalmitoyl phosphatidylcholine and dimyristoyl phosphatidylcholine, and the phase trans...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01400-5
更新日期:2004-01-02 00:00:00
abstract::Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01294-6
更新日期:1999-10-22 00:00:00
abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00719-4
更新日期:1998-07-10 00:00:00
abstract::Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.049
更新日期:2006-10-02 00:00:00
abstract::Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase P...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00032-4
更新日期:1996-02-19 00:00:00
abstract::Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80715-7
更新日期:1993-11-22 00:00:00
abstract::Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81301-f
更新日期:1993-02-01 00:00:00
abstract::Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80238-c
更新日期:1992-03-09 00:00:00
abstract::Aminopeptidase yscXVI was purified from the yeast Saccharomyces cerevisiae. By SDS-PAGE the enzyme has a molecular weight of 45,000 Da, and in chromatofocusing, elution was observed at pH 6.2. The synthetic substrate cystinyl-4-nitroanilide (Km 22.5 microM, Vmax 12.9 mU/mg) is cleaved most efficiently in the pH range ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81566-i
更新日期:1993-05-10 00:00:00
abstract::Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02862-9
更新日期:2001-09-21 00:00:00
abstract::This study describes for the first time the amphiphilicity of the protein moiety of proteoglycogen. Glycogenin but not proteoglycogen associates to phospholipid vesicles and forms by itself stable Gibbs and Langmuir monolayers at the air-buffer interface. The adsorption free energy (-6.7 kcal/mol) and the glycogenin c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03192-1
更新日期:2001-12-07 00:00:00
abstract::Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80479-9
更新日期:1987-11-30 00:00:00
abstract::On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The At...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01083-2
更新日期:1999-09-03 00:00:00
abstract::Disruption of yeast spheroplasts by DEAE-dextran in isoosmotic conditions allows isolation of relatively undamaged subcellular fractions from yeast. The preservation of mitochondria and vacuoles permits the simultaneous isolation of the cytosol with negligible contamination by vacuolar proteinases and therefore, virtu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80512-2
更新日期:1983-06-13 00:00:00
abstract::Pyruvate dehydrogenase (E1p) is one of the components of the pyruvate dehydrogenase multienzyme complex (PDHC). Previously, it was shown that the N-terminal domain of E1p is involved in its binding to the core component (E2p) of PDHC. We constructed point mutations in this domain (D17Q, D17R, E20Q, E20R, D24Q and D24R...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02931-9
更新日期:2002-07-03 00:00:00
abstract::The nucleotide binding protein 35 (Nbp35)/cytosolic Fe-S cluster deficient 1 (Cfd1)/alternative pyrimidine biosynthetic protein C (ApbC) protein homologs have been identified in all three domains of life. In eukaryotes, the Nbp35/Cfd1 heterocomplex is an essential Fe-S cluster assembly scaffold required for the matura...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13673
更新日期:2020-03-01 00:00:00
abstract::The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00581-x
更新日期:1998-06-12 00:00:00
abstract::Escherichia coli evolves H2 via multiple [Ni-Fe]-hydrogenases (Hyd). This activity under hyper- and hypo-osmotic stress was investigated with mutants lacking different Hyd enzymes during glycerol fermentation. Inhibitory effects of hypo-stress on H2 production was stronger at pH 6.5 in wild type and mutants except fhl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.016
更新日期:2013-11-01 00:00:00
abstract::The action of substrate analogs containing the tetrazolyl group instead of the C-terminal carboxy group on the peptidase activity of carboxypeptidase A is studied. The analogs compete with the substrate for the secondary binding site thus showing activation phenomena. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81561-2
更新日期:1987-02-09 00:00:00
abstract::Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81164-8
更新日期:1983-10-31 00:00:00
abstract::Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80661-x
更新日期:1985-05-06 00:00:00
abstract::The rate of mitochondrial ATPase inactivation by the naturally occurring inhibitor protein in the presence of saturating ATP and Mg2+ at pH 8.0 depends hyperbolically on the amount of inhibitor added; the upper limit of an apparent first-order constant for the inactivation process is 1.0(-1) at 25 degrees C. A dramati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80611-6
更新日期:1985-05-20 00:00:00
abstract::The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran-induced, phosphatidylserine-dependent degranulation of rat peritoneal mast cells. The dextran-induced Ca2+-uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca2+-uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80725-x
更新日期:1983-09-05 00:00:00
abstract::The amino acid sequence of the zinc-requiring beta-lactamase II from Bacillus cereus strain 569 has been determined. It consists of a single polypeptide chain of 227 residues. It is the only example so far fully characterized of a class B beta-lactamase, and is structurally and mechanistically distinct from both the w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81024-3
更新日期:1985-09-23 00:00:00
abstract::Platelet release products and purified platelet-derived growth factor stimulated the phosphorylation of ribosomal protein S6 in cultured mouse Balb/c 3T3 fibroblasts. The post-nuclear fraction of the stimulated cells was enriched in S6 kinase activity specific for sites resembling those phosphorylated within intact ce...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81072-2
更新日期:1983-10-03 00:00:00
abstract::Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00400-0
更新日期:1996-05-06 00:00:00
abstract::Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae. To gain information on sphingobase-induced signaling, we determined kinetics of leaf reactive oxyg...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.016
更新日期:2011-10-03 00:00:00
abstract::Mouse interferon (alpha + beta) given to mice by intraperitoneal injection suppressed both the accumulation of putrescine and stimulation of DNA synthesis in liver caused by partial hepatectomy. The suppression of DNA synthesis was completely reversed by exogenous putrescine. The same results were obtained when core 2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80866-3
更新日期:1986-09-01 00:00:00
abstract::APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.032
更新日期:2011-02-18 00:00:00