Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana.

abstract：:A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...

journal_title：FEBS letters

authors： Prestle J,Hornung E,Schönfelder M,Mundry KW

更新日期：1992-02-10 00:00:00

abstract：:IGF-I receptors were partially purified from red and white skeletal muscle by lectin-affinity chromatography and the resultant fraction was depleted of insulin receptors by insulin affinity chromatography. Equilibrium binding of 125I-IGF-I to receptor preparations from red and white muscle yielded identical Scatchard ...

journal_title：FEBS letters

authors： Zorzano A,James DE,Ruderman NB,Pilch PF

更新日期：1988-07-18 00:00:00

abstract：:We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficie...

journal_title：FEBS letters

authors： Rey B,Roussel D,Teulier L,Eyenga P,Degletagne C,Belouze M,Duchamp C

更新日期：2011-01-03 00:00:00

abstract：:Amino acid residues 3-15 of mycobacterial HSP60 define a dominant T-cell epitope for HLA-DR3+ve humans and Mamu-DR3+ve rhesus monkeys. Our results show that Mamu-DR3 molecules on PBMC can be efficiently loaded in vivo with the above-mentioned peptides when they are intravenously injected encapsulated in liposomes, but...

journal_title：FEBS letters

authors： t Hart BA,Elferink DG,Drijfhout JW,Storm G,van Blooijs L,Bontrop RE,de Vries RR

更新日期：1997-06-02 00:00:00

abstract：:We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing the target sequences A8XA8.T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8.T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8. ...

journal_title：FEBS letters

authors： Chandler SP,Fox KR

更新日期：1993-10-11 00:00:00

abstract：:A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respiromet...

journal_title：FEBS letters

authors： Hoeks J,Briedé JJ,de Vogel J,Schaart G,Nabben M,Moonen-Kornips E,Hesselink MK,Schrauwen P

更新日期：2008-02-20 00:00:00

abstract：:Adequate dietary intake of essential metals such as zinc is important for maintaining homeostasis. Abnormal zinc intake in Caenorhabditis elegans has been shown to increase or decrease normal lifespan by influencing the insulin/IGF-1 pathway. Distribution of zinc is achieved by a family of highly conserved zinc transp...

journal_title：FEBS letters

authors： Novakovic S,Molesworth LW,Gourley TE,Boag PR,Davis GM

更新日期：2020-05-01 00:00:00

abstract：:The crystal structure of human immunodeficiency virus type 1 (HIV-1) protease mutant G48H with peptidic inhibitor U-89360E is described. Comparison with wild-type protease-inhibitor complex shows that mutation of flap residue 48 to histidine allows stabilizing van der Waals contacts between the side chains of His48 an...

journal_title：FEBS letters

authors： Hong L,Zhang XJ,Foundling S,Hartsuck JA,Tang J

更新日期：1997-12-22 00:00:00

abstract：:We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

journal_title：FEBS letters

authors： Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

更新日期：2001-11-23 00:00:00

abstract：:LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

journal_title：FEBS letters

authors： Doucet-Beaupré H,Ang SL,Lévesque M

更新日期：2015-12-21 00:00:00

abstract：:Cells of Saccharomyces cerevisiae were loaded with indo-1, by incubation in a medium of pH 4.5, which contained penta-potassium indo-1. Cells were then washed and resuspended in a buffer of pH 4.0. The emission fluorescence spectra were recorded between 390 and 500 nm (excitation at 355 nm) and the autofluorescent spe...

journal_title：FEBS letters

authors： Halachmi D,Eilam Y

更新日期：1989-10-09 00:00:00

abstract：:We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

journal_title：FEBS letters

authors： Sakaguchi M,Honda S,Ozawa M,Nishimura O

更新日期：1988-03-28 00:00:00

abstract：:Proteins implicated as intracellular chloride channels include the intracellular ClC proteins, the bestrophins, the cystic fibrosis transmembrane conductance regulator, the CLICs, and the recently described Golgi pH regulator. This paper examines current hypotheses regarding roles of intracellular chloride channels an...

journal_title：FEBS letters

authors： Edwards JC,Kahl CR

更新日期：2010-05-17 00:00:00

abstract：:FabH (beta-ketoacyl-acyl carrier protein synthase III) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. We report the crystal st...

journal_title：FEBS letters

authors： Gajiwala KS,Margosiak S,Lu J,Cortez J,Su Y,Nie Z,Appelt K

更新日期：2009-09-03 00:00:00

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00

abstract：:Purified plasma membrane vesicles isolated from multidrug-resistant human KB-V1 cells accumulate [3H]vinblastine in an energy-dependent manner. The accumulation of [3H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most effic...

journal_title：FEBS letters

authors： Lelong IH,Padmanabhan R,Lovelace E,Pastan I,Gottesman MM

更新日期：1992-06-15 00:00:00

abstract：:To better understand the outcome of the interaction between TNF-related apoptosis-inducing factor (TRAIL) and tumor cells, we studied TRAIL-resistant melanoma cells resulting from prolonged exposure to TRAIL and found that they had higher proliferative activity than the parental cells both in vitro and in vivo. This w...

journal_title：FEBS letters

authors： Wu JJ,Zhang XD,Gillespie S,Hersey P

更新日期：2005-03-28 00:00:00

abstract：:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...

journal_title：FEBS letters

authors： Abe M,Hashimoto H,Yoda K

更新日期：1999-09-24 00:00:00

abstract：:Phototaxis in the archaeon Halobacterium salinarium is mediated by a stable complex of the photoreceptor sensory rhodopsin I and its transducer HtrI, which relays the light stimulus to the signalling pathway. Removal of the cytoplasmic signalling domain of HtrI eliminated the SRI-specific motor response to light stimu...

journal_title：FEBS letters

authors： Krah M,Marwan W,Oesterhelt D

更新日期：1994-10-24 00:00:00

abstract：:Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...

journal_title：FEBS letters

authors： Maehama T,Kosaka N,Okahara F,Takeuchi K,Umeda M,Dixon JE,Kanaho Y

更新日期：2004-05-07 00:00:00

abstract：:Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

journal_title：FEBS letters

authors： Eckes B,Mauch C,Hüppe G,Krieg T

更新日期：1993-03-01 00:00:00

abstract：:Carboxylesterase (EII') from Arthrobacter sp. KI72 has 88% homology to 6-aminohexanoate-dimer hydrolase (EII) and possesses ca. 0.5% of the level of 6-aminohexanoate-linear dimer (Ald)-hydrolytic activity of EII. To study relationship between Ald-hydrolytic and esterolytic activities, random mutations were introduced ...

journal_title：FEBS letters

authors： Ohki T,Wakitani Y,Takeo M,Yasuhira K,Shibata N,Higuchi Y,Negoro S

更新日期：2006-09-18 00:00:00

abstract：:Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

journal_title：FEBS letters

authors： Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

更新日期：2005-08-15 00:00:00

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Kowaltowski AJ,Vercesi AE,Rhee SG,Netto LE

更新日期：2000-05-12 00:00:00

abstract：:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...

journal_title：FEBS letters

authors： Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJ

更新日期：2007-02-20 00:00:00

abstract：:A novel rapid polymerase chain reaction (PCR)-based technique for N-terminal attachment of green fluorescent protein (GFP) to a yeast protein is described. Genomic integration of a PCR-generated loxPkanMX4loxP-yEGFP fusion cassette immediately upstream of the open reading frame (ORF) allows for selection of G418 resis...

journal_title：FEBS letters

authors： Prein B,Natter K,Kohlwein SD

更新日期：2000-11-17 00:00:00

abstract：:We applied 1D homonuclear Hartmann-Hahn (1D-HOHAHA) and difference NOE experiments to determine the chemical structure of Forssman's antigen, a glycolipid purified from sheep red blood cells. The subspectra corresponding to the individual sugar components were extracted from overlapping proton resonances by selective ...

journal_title：FEBS letters

authors： Inagaki F,Kodama C,Suzuki M,Suzuki A

更新日期：1987-07-13 00:00:00

abstract：:The rotary motion in response to ATP hydrolysis of the ring of c subunits of the membrane portion, F(o), of ATP synthase, F(o)F(1), is still under contention. It was studied with EF(o)EF(1) (Escherichia coli) using microvideography with a fluorescent actin filament. To overcome the limited specificity of actin attachm...

journal_title：FEBS letters

authors： Pänke O,Gumbiowski K,Junge W,Engelbrecht S

更新日期：2000-04-21 00:00:00

abstract：:As a SNARE binding protein, tomosyn has been reported to negatively regulate synaptic exocytosis via arresting syntaxin-1 and SNAP-25 into a nonfusogenic product that precludes synaptobrevin-2 entry, raising the question how the assembly of the SNARE complex is achieved. Here, we have investigated new functions of tom...

journal_title：FEBS letters

authors： Li Y,Wang S,Li T,Zhu L,Ma C

更新日期：2018-04-01 00:00:00