Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

abstract：:Smad ubiquitination regulatory factor 2 (Smurf2), a ubiquitin ligase for Smads, plays critical roles in the regulation of transforming growth factor-beta (TGF-beta)-Smad signaling via ubiquitin-dependent degradation of Smad2 and Smad7. We found that TGF-beta stimulates Smurf2 expression. TGF-beta activated the Smurf2 ...

journal_title：FEBS letters

authors： Ohashi N,Yamamoto T,Uchida C,Togawa A,Fukasawa H,Fujigaki Y,Suzuki S,Kitagawa K,Hattori T,Oda T,Hayashi H,Hishida A,Kitagawa M

更新日期：2005-05-09 00:00:00

abstract：:IscA homologs are known to be involved in iron-sulfur cluster formation in various organisms. Recombinant proteins of two IscA homologs from the cyanobacterium Synechocystis PCC 6803, designated SLR1417 and SLR1565, were purified. The absorption spectrum of purified SLR1565 was typical for [2Fe-2S] cluster-containing ...

journal_title：FEBS letters

authors： Morimoto K,Nishio K,Nakai M

更新日期：2002-05-22 00:00:00

abstract：:Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

journal_title：FEBS letters

authors： Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

更新日期：1991-08-05 00:00:00

abstract：:The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP...

journal_title：FEBS letters

authors： Lin HY,Yu CY,Hsu YH,Meng M

更新日期：2012-07-30 00:00:00

abstract：:Myeloma cells specifically localize in the bone marrow and rarely circulate in blood. To study whether this immobilization could be partially explained by the presence of constitutively activated integrins, particularly alpha4beta1, we used the activation reporter HUTS-21 anti-beta1 mAb. These analyses showed that bet...

journal_title：FEBS letters

authors： García-Gila M,Cabañas C,García-Pardo A

更新日期：1997-12-01 00:00:00

abstract：:Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stim...

journal_title：FEBS letters

authors： Roumanie O,Weinachter C,Larrieu I,Crouzet M,Doignon F

更新日期：2001-10-05 00:00:00

abstract：:Interrogation of the Human Genome data for sequences related to the von Willebrand factor A-domain module identified a previously unreported 4.1 kb full-length cDNA that is predicted to encode a new member of the collagen superfamily of extracellular matrix proteins, collagen XXI. The domain organization of collagen X...

journal_title：FEBS letters

authors： Fitzgerald J,Bateman JF

更新日期：2001-09-14 00:00:00

abstract：:ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...

journal_title：FEBS letters

authors： Jones CP,Khan K,Ingram-Smith C

更新日期：2017-02-01 00:00:00

abstract：:In HL-60 cells signal transduction via sphingomyelin hydrolysis (sphingomyelin cycle) is induced by binding of tumor necrosis factor alpha (TNFalpha) to cell surface TNFalpha receptor. We found that IgG immunoglobulins activate sphingomyelin hydrolysis in plasma membrane of HL-60 cells, with kinetics similar to that o...

journal_title：FEBS letters

authors： Glick D,Barenholz Y

更新日期：1996-10-07 00:00:00

abstract：:NDRG1 is phosphorylated by SGK1 (but not PKB) in vivo at three residues each contained within three nonapeptide repeats. Here, we demonstrate that this nonapeptide, like the NDRG1 protein, is phosphorylated by SGK1, but not by PKBalpha or RSK1 in vitro. The inability of PKBalpha and RSK1 to phosphorylate the nonapepti...

journal_title：FEBS letters

authors： Murray JT,Cummings LA,Bloomberg GB,Cohen P

更新日期：2005-02-14 00:00:00

abstract：:Regulatory protein kinases are involved in various cellular processes such as proliferation, differentiation, and apoptosis. Using cDNA differential display, we identified MOK, a member of the mitogen-activated protein kinase superfamily, as one of the genes induced by a caudal-related homeobox transcription factor, C...

journal_title：FEBS letters

authors： Uesaka T,Kageyama N

更新日期：2004-08-27 00:00:00

abstract：:Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...

journal_title：FEBS letters

authors： Shiroshima T,Oka C,Kawaichi M

更新日期：2009-01-05 00:00:00

abstract：:The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...

journal_title：FEBS letters

authors： Kotelnikova RA,Kotelnikov AI,Bogdanov GN,Romanova VS,Kuleshova EF,Parnes ZN,Vol'pin ME

更新日期：1996-07-01 00:00:00

abstract：:We report results from automated docking and microscopic molecular dynamics simulations of the tetraethylammonium (TEA) complexes with KcsA. Binding modes and energies for TEA binding at the external and internal sides of the channel pore are examined utilising the linear interaction energy method. Effects of the chan...

journal_title：FEBS letters

authors： Luzhkov VB,Aqvist J

更新日期：2001-04-27 00:00:00

abstract：:Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...

journal_title：FEBS letters

authors： Yoo SH,Ferretti JA

更新日期：1993-11-22 00:00:00

abstract：:Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...

journal_title：FEBS letters

authors： Esposito C,Carullo P,Pedone E,Graziano G,Del Vecchio P,Berisio R

更新日期：2010-03-19 00:00:00

abstract：:The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

journal_title：FEBS letters

authors： Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

更新日期：1991-11-04 00:00:00

abstract：:Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a ...

journal_title：FEBS letters

authors： Ikawa M,Kominami K,Yoshimura Y,Tanaka K,Nishimune Y,Okabe M

更新日期：1995-11-13 00:00:00

abstract：:The phosphopeptide Ser (P)-Ser(P)-Ser-(P)-Glu-Glu-Ser22-Ile-Thr, reproducing the 17-24 segment of beta-casein A2 including the seryl residue (Ser-22) which is targeted by casein kinase-1 was synthesized and used as model substrate for this enzyme. Its phosphorylation efficiency is actually higher than that of intact b...

journal_title：FEBS letters

authors： Meggio F,Perich JW,Reynolds EC,Pinna LA

更新日期：1991-06-03 00:00:00

abstract：:Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

journal_title：FEBS letters

authors： Hofmann S,Düsterhöft S,Pette D

更新日期：1988-10-10 00:00:00

abstract：:In higher plants, two types of nitrate transporters, NRT1 and NRT2, have been identified. In Arabidopsis, there are 53 NRT1 genes and 7 NRT2 genes. NRT2 are high-affinity nitrate transporters, while most members of the NRT1 family are low-affinity nitrate transporters. The exception is CHL1 (AtNRT1.1), which is a dual...

journal_title：FEBS letters

authors： Tsay YF,Chiu CC,Tsai CB,Ho CH,Hsu PK

更新日期：2007-05-25 00:00:00

abstract：:Cerebral deposits of beta/A4 amyloid protein is a pathologic sign of Alzheimer's disease. A synthetic partial-length (1-28) peptide of this protein contains one glutamine and two lysine residues. Here we show that this peptide can be a substrate of transglutaminase, which catalyzes cross-linking between glutamine and ...

journal_title：FEBS letters

authors： Ikura K,Takahata K,Sasaki R

更新日期：1993-07-12 00:00:00

abstract：:We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released wi...

journal_title：FEBS letters

authors： Babinski K,Féthière J,Roy M,De Léan A,Ong H

更新日期：1992-11-30 00:00:00

abstract：:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

journal_title：FEBS letters

authors： Durchschlag H,Zipper P

更新日期：1988-09-12 00:00:00

abstract：:Apolipoprotein E (APOE) genotype determines Alzheimer's disease (AD) susceptibility, with the APOE ε4 allele being an established risk factor for late-onset AD. The ApoE lipidation status has been reported to impact amyloid-beta (Aβ) peptide metabolism. The details of how lipidation affects ApoE behavior remain to be ...

journal_title：FEBS letters

authors： Hubin E,Verghese PB,van Nuland N,Broersen K

更新日期：2019-06-01 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...

journal_title：FEBS letters

authors： Murányi A,Zhang R,Liu F,Hirano K,Ito M,Epstein HF,Hartshorne DJ

更新日期：2001-03-30 00:00:00

abstract：:The p53 tumor suppressor coordinates a multitude of cellular and organismal processes and exerts its activities mainly by activation of gene transcription. Here we describe the transcriptional activation of ectodysplasin A2 receptor (EDA2R) by p53 in a variety of cell types and tissues. We demonstrate that treatment o...

journal_title：FEBS letters

authors： Brosh R,Sarig R,Natan EB,Molchadsky A,Madar S,Bornstein C,Buganim Y,Shapira T,Goldfinger N,Paus R,Rotter V

更新日期：2010-06-03 00:00:00

abstract：:The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...

journal_title：FEBS letters

authors： Do SI,Lee KY

更新日期：1998-01-09 00:00:00

abstract：:The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

journal_title：FEBS letters

authors： Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

更新日期：1984-01-23 00:00:00

abstract：:Vasopressin elicited a dose-dependent inhibition of glucagon-induced cAMP accumulation in isolated hepatocytes. This response was not diminished by incubation of cells with the calmodulin antagonists trifluoperazine or chlorpromazine and was only slightly reduced in Ca2+-depleted hepatocytes. Half-maximal inhibition o...

journal_title：FEBS letters

authors： Morgan NG,Shipp CC,Exton JH

更新日期：1983-11-14 00:00:00